Tài liệu Báo cáo khoa học: Use of hydrostatic pressure to produce ‘native’ monomers of yeast enolase ppt

... Use of hydrostatic pressure to produce ‘native’ monomers of yeast enolase M. Judith Kornblatt 1 , Reinhard Lange 2, * and Claude Balny 2, * 1 Department of Chemistry and Biochemistry, ... had to be bound to the enzyme. This article presents a study of the pressure- induced dissociation of yeast enolase in the presence of Mn 2+ .We demonstrate the abili...

Ngày tải lên: 19/02/2014, 16:20

... how knowing the sort of each character is use- ful when tokenizing a string of characters into a string of words and when assigning parts -of- speech to them, and our method of clustering characters ... of their distributions with respect to a presumably natural class called numericals. Supposing one of a certain group of characters often occurs as a neighbor to o...

Ngày tải lên: 20/02/2014, 18:20

... 11cRAL to cone photoreceptors in cone-dominant species. Identification of an alterna- tive visual cycle will contribute to the understanding of the functional differ- ences of rod and cone photoreceptors. Structured ... absence of a metal chelator, RPE65c cat- alyzed the production of 11cROL and 13cROL from atRE (Fig. 4A). However, in the presence of 1 m M of the metal chelat...

Ngày tải lên: 14/02/2014, 14:20

... doublet of ITK fuses directly with the linker B kinase region of SYK. The PH domain of TFKs usually binds to phosphatidylino- sitol-3,4,5-trisphosphate, thereby bringing them in close proximity to ... 1–196) of BTK fused with the linker B kinase region of SYK (306–635 amino acids) (Fig. 2). We used two different cell types to study the phosphorylation status of key residues...

Ngày tải lên: 14/02/2014, 18:20

... supply to hedge against periods of food shortage. Today, humans have pushed storage too far, to the point of widespread obesity. Although obesity is preferable to starvation, this state frequently ... regulatory step of glycolysis catalyzed by phosphofructokinase, which is used by glucose, but not fructose. This rapidly activates AMPK rather than inhibiting AMPK as glucose does. T...

Ngày tải lên: 14/02/2014, 22:20

... induces apoptosis in a Bax-dependent manner. Inhibitor of apoptosis protein family Induction of MOMP following apoptotic stimuli results in the release of cytochrome c and subsequent activation of caspase-9 ... characterized by the permeabili- zation of the outer mitochondrial membrane and the release of several pro-apoptotic factors into the cytoplasm. For mitochondrial outer mem...

Ngày tải lên: 16/02/2014, 09:20

... and resistance to apoptosis through downregulation of tBid, providing further details on how EGF receptor and proteasome inhibitors can contribute to the induction of apoptosis and the treatment of cancer. Structural ... changes that occur during apoptosis. The mitochondrial release of cytochrome c and second mitochondria-derived activator of caspase (Smac ⁄ DIABLO) allows for t...

Ngày tải lên: 16/02/2014, 09:20

... each form: s i ¼½S i =S tot ; s ¼½S=S tot ; s 1 ¼½S a1 =S tot ; s 2 ¼½S a2 =S tot ð5Þ The conservation of the total Src concentration (Eqn 4) leaves only three independent variables in the kinetic scheme of Fig. ... com- plex dynamics of Src responses can be understood in terms of a simple basic model of the Src (de)activation cycle in the absence of any imposed external feedb...

Ngày tải lên: 18/02/2014, 11:20

... appear to be due to Pgp activity. Because the over-expression of MRP 1–5 did not significantly reduce the amount of TMRM taken up into the cells, the obstacle to uptake of rhodamines into the ... the total dye amount associated with the cells, including the dye adsorbed to the sur- face of the cells. To avoid complication of the uptake studies by active pumping of the dye...

Ngày tải lên: 18/02/2014, 13:20

... system in which Ab was fused to ubiquitin. This protocol relies on the use of Ni-NTA affinity chromatography for purification and subse- quent liberation of Ab by digestion using yeast ubiqu- itin hydrolase, ... solution. To minimize carbam- ylation of Ab, fresh solutions of ice-cold, deionized ACS grade urea were used, and the duration of exposure to urea was limited to l...

Ngày tải lên: 18/02/2014, 13:20