... amounts of recombinant proteins onto the gel, which are detected by the same antibody as the protein of interest. Usingmicroscopic techniques, the volume of a cell can beestimated, allowing conversion ... the protein, cross-reaction with other proteins resulting in a highbackground, or by the use of generic phosphotyrosineantibodies. In such cases, the protein of interest has tobe prepurified by ... in the hematopoietic suspensioncell line, BaF3-hemaglutinin-tagged (HA)-EpoR, and the interleukin-6 (IL-6)-induced activation of the signaltransducer and activator of transcription (STAT)3 in adherent...