Tài liệu Báo cáo khoa học: Selection of stably folded proteins by phage-display with proteolysis docx

... MINIREVIEW Selection of stably folded proteins by phage-display with proteolysis Yawen Bai and Hanqiao Feng Laboratory of Biochemistry, National Cancer Institute, ... with the desired properties from libraries of mutant proteins have been sought [5–8]. Phage-display coupled with proteolysis for selection of stably folded proteins was one such recently...

Ngày tải lên: 19/02/2014, 12:20

... acid sequence of mature cathep- sin B (Fig. S1) were synthesized on the spots of cellu- lose membrane. After incubation of the membrane with 2A2 mAb, followed by the detection with second- ary ... the presence of various concentrations of 2A2 mAb. The binding of 2A2 mAb was followed by native gel elec- trophoresis. Increasing the concentration of 2A2 mAb at a fixed molar...

Ngày tải lên: 18/02/2014, 11:20

... kinetics of a-synuc- lein. When a-synuclein was incubated with MPTP, the rate of formation of the more toxic protofibrils (mea- sured as lag time) was delayed, whereas the rate of conversion of protofibrils ... solu- tion of ThT and the fluorescence intensity of the solu- tion was measured at 482 nm. Figure 4A shows the kinetics of aggregation of a-synuclein in the presence...

Ngày tải lên: 14/02/2014, 19:20

... hydrolysis of triolein by purified recombinant rat adipocyte HSL, with a decrease in the effect of bis-ANS upon PKA phosphorylation of HSL. The interaction of HSL with bis-ANS was found to have a K d of ... interac- tion with HSL is altered upon phosphorylation of HSL by PKA (Fig. 2). HSL interaction with bis-ANS To evaluate the binding of bis-ANS to nonphosphory- lat...

Ngày tải lên: 18/02/2014, 11:20

... presence of this enzyme in different tissues with properties of endothelial NOS, nNOS, and ⁄ or iNOS [14,31,32]. In different models of sepsis and tissues, the existence of both mtNOS and i-mtNOS isoforms ... decay pathway of NO is its reaction with O 2 – to form ONOO – [20]. In turn, ONOO – reacts with a variety of biomolecules [35]. Moreover, ONOO – can react with H4-bio...

Ngày tải lên: 19/02/2014, 00:20

... together chitinases and proteins devoid of catalytic activity due to the substitution of a critical amino acid in the cata- lytic centre. This latter singular class of proteins, called chitinase-like proteins ... mean + SE of three pools of four animals (tissues) or the mean of one pool of embryos or larva from one spawn. Expression levels are related to 100 copies of GAP...

Ngày tải lên: 19/02/2014, 00:20

... Effect of Decma on Shc phosphorylation and its association with Grb2. After treat- ment of cells with Decma supernatant for 30 min, 300 lg of total cell lysates were immunoprecipitated with anti-Shc ... indicated, followed by western blotting for Shc, phospho-Erk and total Erk levels. (C) Rescue by ectopic Shc isoform expression of Erk activation that was suppressed by dow...

Ngày tải lên: 19/02/2014, 05:20

... these proteins stimulate some of its activities because of better substrate availability is not yet known. The partial restoration of proteasome activities by Hsps contrasts with the lack of effect induced ... expansion- dependent manner, with an increased level of intracel- lular ROS and with increased formation of carbonyl residues in proteins. The enhanced oxidation...

Ngày tải lên: 19/02/2014, 06:20

... synthesis of the different RNAs were obtained by PCR amplification of the first 125 nucleotides of the rpsO mRNA (5¢rpsO), the last 97 nucleotides of the same transcript (3¢rpsO), part of the 5¢ UTR of ... by PAP I M. Folichon et al. 456 FEBS Journal 272 (2005) 454–463 ª 2004 FEBS AB Fig. 2. Hfq stimulates addition of A residues by PAP I but not of C residues. (A) Addition...

Ngày tải lên: 19/02/2014, 16:20

... order rates of association (k ass ) values for the reaction of serpins with proteases in the presence and absence of a range of GAGs (values are representative of those reported in a range of publications ... many of the principal procoagulant compo- nents of the system are serine proteases, regulation of the system is principally by the action of serine protease inhib...

Ngày tải lên: 20/02/2014, 02:21