Tài liệu Báo cáo khoa học: Preliminary molecular characterization and crystallization of mitochondrial respiratory complex II from porcine heart ppt

... N-terminal sequencing and crystallization of mitochondrial com- plex II from porcine heart. Results and Discussion The mitochondrial respiratory complex II preparation was extracted and purified from porcine heart. ... integral part of the complex [18]. The crystal structure of mitochondrial respiratory complex II has been determined at 2.4 A ˚ f...

Ngày tải lên: 19/02/2014, 02:20

... 3-phenylindole on the activity of BH from mycelial cultures of Leptosphaeria maculans (A) and Alternaria brassicicola (B). The results are expressed as means and standard deviations of three independent ... 85% inhibition of BHLmL2 and BHAb, respectively, while 1 mm 2-mer- captoethanol caused 85% and 97% inhibition of BHLmL2 and BHAb, respectively. The influence of pH o...

Ngày tải lên: 18/02/2014, 14:20

... 23S.5DG b pre-RNA, and the binding of a minimum of n Mg 2+ ions leads to formation of the active complex, whereas binding of a minimum of m Mn 2+ ions forms the inactive complex. The sizes of the active and ... Herrin 2 1 Department of Biochemistry, Tapei Medical University, Taiwan 2 Section of Molecular Cell and Developmental Biology and Institute for Cellular an...

Ngày tải lên: 19/02/2014, 07:20

... natural and disulfide-mispaired, with the sequence of a-conotoxin SI were described [53]. (Disulfide isomers: natural I-III, II- IV; mispaired ÔnestedÕ I-IV, II- III and mispaired ÔdiscreteÕ I -II, III-IV, ... information for ImIIA and Vc1.1, ImII, GIC and PIA, each of which was identified by prediction from a genomic DNA clone [7–10,22]. Comparison of the peptide sequences GI...

Ngày tải lên: 19/02/2014, 12:20

... conclusions from the phylogeny. The present study cannot exclude that clades I and II could have a common origin with cytosolic CAI, CAII, CAIII, CAVII and mitochondrial CAV vertebrate isoforms, ... overexpression approach of RpCAbr and RpCAtr, we cannot know the functional effect of changes of some key amino-acids. Origin and number of nonvertebrate CAs Although the boo...

Ngày tải lên: 18/02/2014, 16:20

... (A) Expanded view of peaks at m ⁄ z 2800.36 and 2832.35 and the fragmentation of the peak at m ⁄ z 2800.36. (B) Expanded view of peaks at m ⁄ z 2934.36 and 2950.35 and the fragmentation of the ... ratio of 8 : 2, spotted onto the Anchor-Chip plate and air dried. Peptide spectra over a mass range of m ⁄ z 500–3500 and MS ⁄ MS spectra of select ions were collected on a...

Ngày tải lên: 18/02/2014, 17:20

... the number of cuts in each direction from the center, and m (=2), the minimum length of each part. Both halves are classified separately, and if the maximum anglicism classifier score out of all splits ... comparison of D U , D T , log 10 f with ∆ log 10 f quency (log 10 f). D U = U w ˜ U w is defined as the ratio of the actual number of users of word w (U w ) over the expect...

Ngày tải lên: 19/02/2014, 19:20

Ngày tải lên: 20/02/2014, 18:20

... recognition and classification, etc.); (ii) parsing and naive semantic interpretation; (iii) inference; (iv) triple extraction. The rest of this paper describes our method for indexing and retrieval ... With, firearm: revolver) • (ii) (firearm : revolver, In, part of dwelling kitchen) • (iii) (cutting instrument : knife, In, part of dwelling : kitchen) where triple (iii) was i...

Ngày tải lên: 22/02/2014, 02:20

... units of activity of the purified (lanes 1, 3 and 5) and recombinant (lanes 2, 4 and 6) enzymes were used. Lanes 1 and 2, E. quadrivirgata enzyme; lanes 3 and 4, E. climacophora enzyme; lanes 5 and ... Leu130 and Leu166, of Fig. 2. Alignment of the amino acid sequences of snake DNases I with those of human, chicken and X. laevis DNases I. The aa sequences of the s...

Ngày tải lên: 20/02/2014, 23:20