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Tài liệu Báo cáo khoa học: DNA adenine methylation changes dramatically during establishment of symbiosis pdf

Tài liệu Báo cáo khoa học: DNA adenine methylation changes dramatically during establishment of symbiosis pdf

Tài liệu Báo cáo khoa học: DNA adenine methylation changes dramatically during establishment of symbiosis pdf

... adenine methylation H. Ichida et al.962 FEBS Journal 274 (2007) 951–962 ª 2007 The Authors Journal compilation ª 2007 FEBS DNA adenine methylation changes dramatically during establishment of ... bacter-ial DNA adenine methylation status changes during the establishment of the symbiotic relationship andmay contribute to the regulation of plant–microbeinteractions.To confirm the change in methylation ... December2006)doi:10.1111/j.1742-4658.2007.05643.xThe DNA adenine methylation status on specific 5¢-GANTC-3¢ sites andits change during the establishment of plant–microbe interactions wasdemonstrated in several species of a-proteobacteria....
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Tài liệu Báo cáo khoa học: Moult cycle-related changes in biological activity of moult-inhibiting hormone (MIH) and crustacean hyperglycaemic hormone (CHH) in the crab, Carcinus maenas From target to transcript ppt

Tài liệu Báo cáo khoa học: Moult cycle-related changes in biological activity of moult-inhibiting hormone (MIH) and crustacean hyperglycaemic hormone (CHH) in the crab, Carcinus maenas From target to transcript ppt

... firstinvestigated the biological activity of MIH and CHH during precisely timed stages of the moult cycle of Carcinusto determine changes in: (a) potency of these peptides inrepressing ecdysteroid ... reduction in MIH content of theSG, relative to CHH, during late premoult.Expression patterns of MIH and CHH in the X-organ during the moult cycleExpression patterns of mRNA levels of both MIH andCHH ... receptor number during postmoult wasnot observed. Thus, during postmoult, a significant recruit-ment of receptors for MIH was suggested.Levels of MIH and CHH in the sinus gland during the moult...
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Tài liệu Báo cáo khoa học: DNA methylation-mediated nucleosome dynamics and oncogenic Ras signaling pptx

Tài liệu Báo cáo khoa học: DNA methylation-mediated nucleosome dynamics and oncogenic Ras signaling pptx

... groups on DNA affect the structure of DNA and the interaction of other proteins and enzymes with local nucleosomes [2,60]. Methylation of DNA (MeCpG-) brings about a general hydra-tion of DNA [61], ... between depletion of H3K4Me2 and regions of DNA methylation, and pro-posed that DNA methylation dictates a closed chroma-tin structure devoid of H3K4Me2 [59]. The recentdiscovery of histone demethylases ... disease. DNA methylation enzymesIn mammalian cells, DNA methylation is catalyzedby two classes of DNMT. DNA- methyltransferase-1(DNMT1; EC 2.1.1.37) is essential for maintaining DNA methylation...
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Tài liệu Báo cáo khoa học: DNA modification with cisplatin affects sequence-specific DNA binding of p53 and p73 proteins in a target site-dependent manner pptx

Tài liệu Báo cáo khoa học: DNA modification with cisplatin affects sequence-specific DNA binding of p53 and p73 proteins in a target site-dependent manner pptx

... targets).Effects of DNA cis-platination on sequence-specific DNA binding of p73 proteinsWe tested the influence of DNA modification withcisplatin on the binding of two p73 isoforms, p73d andp73b, ... stability of the p53 DNA complexes [10].In addition, interactions of the p53 protein with cer-tain response elements can be controlled by changes in DNA topology inducing formation of non-B DNA structures ... activity of the drug [26,29,30].Interactions of the p53 protein with cisplatin-modi-fied DNA (cisPt -DNA) have recently been studied [31–36]. In the absence of the p53DBS, enhancement of p53 sequence-nonspecific...
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Tài liệu Báo cáo khoa học: DNA strand exchange activity of rice recombinase OsDmc1 monitored by fluorescence resonance energy transfer and the role of ATP hydrolysis pptx

Tài liệu Báo cáo khoa học: DNA strand exchange activity of rice recombinase OsDmc1 monitored by fluorescence resonance energy transfer and the role of ATP hydrolysis pptx

... lM of OsDmc1 in absence of ATP; (2) with 1.25 lM of OsDmc1 in presence of 2.0 mM of AMP-PNP; (3) with 1.25 lM of OsDmc1 in presence of 2.0 mM ATP-c-S; (4) with 1.25 lM of OsDmc1 in presence of ... reaction. FRET changes wereunaffected by the steps of deproteinization following OsDmc1 reaction,suggesting that the assay results reflected stable events involving exchanges of homologous DNA strands. ... resulting in stretching of DNA by1.5 times its original length. This conformationalchange in DNA is hypothesized to facilitate homologysearch in the double stranded DNA (dsDNA). Oncethe homologous...
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Tài liệu Báo cáo khoa học: Steady-state and time-resolved fluorescence studies of conformational changes induced by cyclic AMP and DNA binding to cyclic AMP receptor protein from Escherichia coli ppt

Tài liệu Báo cáo khoa học: Steady-state and time-resolved fluorescence studies of conformational changes induced by cyclic AMP and DNA binding to cyclic AMP receptor protein from Escherichia coli ppt

... A˚onbinding of cAMP to the anti-cAMP-binding sites of theprotein. The distance between the sulfur atom of Cys178 andthe C9–C10 bond of the indole ring of Trp85, derived fromthe crystal structure of ... thevalue of 0.094 for the CRP–(cAMP)2complex, and nosubstantial changes in the shape of the emission spectra of the donor have been observed, this justifies the lack of thealteration of the ... of the CAP DNA complex at 2.5 A˚resolution: a complete picture of the protein– DNA interface. J. Mol. Biol. 260, 395–408.6. Passner, J.M. & Steitz, T.A. (1997) The structure of a CAP DNA complex...
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Tài liệu Báo cáo khoa học: P25a ⁄ TPPP expression increases plasma membrane presentation of the dopamine transporter and enhances cellular sensitivity to dopamine toxicity pptx

Tài liệu Báo cáo khoa học: P25a ⁄ TPPP expression increases plasma membrane presentation of the dopamine transporter and enhances cellular sensitivity to dopamine toxicity pptx

... equal concentrations of vector DNA wereincubated in the absence and presence of 0.5 mM DA for 24 h andsubjected to analysis of viability by the MTT assay. Co-transfection of DAT with empty vector ... membrane expression of DATA potential effect of p25a on DAT function wasaddressed by expressing DAT alone and in the presence of p25a in HEK-293-MSR cells. To validate the specific-ity of the C-20 ... co-expres-sion of DAT with a-syn was used as a control forprotein over expression. a-syn eliminated the toxicityproduced by addition of DA (Fig. 5).DiscussionDegeneration of the dopaminergic neurons of...
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Tài liệu Báo cáo khoa học: A systems biology approach for the analysis of carbohydrate dynamics during acclimation to low temperature in Arabidopsis thaliana doc

Tài liệu Báo cáo khoa học: A systems biology approach for the analysis of carbohydrate dynamics during acclimation to low temperature in Arabidopsis thaliana doc

... of C24.Doc. S1b. sbml format of model structure of C24.Doc. S2a. Model structure of Rsch.Doc. S2b. sbml format of model structure of Rsch.Doc. S3. Documentation of the model structure.This ... values of parameter sets described metabolic changes during cold exposure within the SDs of experi-mental results (Fig. 5A–D), thus proving reproducibility of the obtained parameters and of simulation ... rate of net starch synthesis (rCO2!Starch)was deter-mined by interpolation of measured starch contents(unit: C6) and calculation of the first derivative of thisfunction. The flux rate of...
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Tài liệu Báo cáo khoa học: Angiopoietin-like proteins: emerging targets for treatment of obesity and related metabolic diseases pptx

Tài liệu Báo cáo khoa học: Angiopoietin-like proteins: emerging targets for treatment of obesity and related metabolic diseases pptx

... induces a state of ‘leptin resistance’in obese animals and humans resulting in development of hyperleptinemia. Similarly, hyperleptinemia is aconsequence of the development of insulin resistancein ... endoplasmic reticulum stress) of adipose tissue. These findings support the possibilitythat the alteration of circulating ANGPTL2 levelscould serve as a marker of obesity-induced metabolicabnormalities. ... humans, visceral fat is one of the pri-mary sources of circulating ANGPTL2. In addition,ANGPTL2 mRNA expression in cultured 3T3-L1adipocytes was halved 24 h after addition of a PPARcagonist to...
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Tài liệu Báo cáo khoa học: Proteolytic processing regulates pathological accumulation in dentatorubral-pallidoluysian atrophy pdf

Tài liệu Báo cáo khoa học: Proteolytic processing regulates pathological accumulation in dentatorubral-pallidoluysian atrophy pdf

... pathogenesis of HD[25]. In the present study, we aimed to elucidate some of details of the proteolytic processing of ATN1 andthe mechanisms of ATN1 accumulation with theexpansion of polyQ. We ... amount of mF2 waslikely caused by the pathological accumulation of ATN1, and was a result of the expansion of the polyQtract. The present immunocytochemical study revealedthat the accumulation of ... cells(5 · 105cells per 100 mm dish) were transfected with 10 lg of ATN1-Q19-or-Q77-pcDNA3.1, 0.1 lgofEmGFP-pcDNA3.1 and 27 lL of FuGENE6, and incubated at37 °C for 48 h. After washing twice...
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