Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse pdf

... FEBS Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse Takashi Yamaguchi, Taeko Ichise, Osamu Iwata, Akiko ... using the following primers: LTA-1F, 5¢-CTC GAGATGGATAAAGTTTTAAACAGAG-3¢ and LTA- 1R, 5¢-TGAAGGCAAATCTCTGGAC-3¢ for the former, and LTA–M2F,...

Ngày tải lên: 18/02/2014, 17:20

... and increased risk of atherosclerosis [21], focal ANGPTL2 secreted by vascular endothelial cells may be a mediator linking smoking to cardiovascular disease in an autocrine or paracrine manner. ... cell migration in vitro and in avascular tissues, such as the cornea [15]. Transgenic mice expressing ANGPTL2 in adipose tissue show vascular inflammation and increased macrophag...

Ngày tải lên: 14/02/2014, 22:20

... demonstrated that Asp129 of NirF could not be essential for any function similar to that in Asp141 of Met8P. The idea of NirF being a dehydrogenase is appealing because of the presence of a putative ... motif in the N-terminal of the protein sequence and also because there is a need for oxidation in the d 1 heme biosynthetic pathway, for example, oxidation...

Ngày tải lên: 15/02/2014, 01:20

... to cathepsin H. To illustrate this, we calculated the aver- age distances between CA atoms of the active site cys- teine and histidine residues in cathepsins B and H and the center of CA atoms of ... structure of the occluding loop residues adapts to each binding ligand in its own way and swings out only as much as is mandatory. Results and Discussion Crystals of...

Ngày tải lên: 18/02/2014, 04:20

... Petersburg, FL, USA) followed by PCR with Taq DNA polymerase (Promega, Madison, WT, USA) using the primers 5¢-CACACTACACTGGGAAGCAGAG ACTCCAGC-3¢ and 5¢-AGCTGGCCCAGCAGCCATACACAGTTAAAG- 3¢. The cDNA was subcloned ... Tomohiro Chiba for preparation of embryonic day 9.5 mouse embryos; Dr Dovie Wylie and Ms Tak- ako Hiraki for expert assistance; and all members of the Departm...

Ngày tải lên: 18/02/2014, 16:20

... focus of the mathematical approach is on the ‘leftward shift’, illustrated in Figs 1 and 2, a statisti- cal analysis was performed for additional justification. The sums of squared deviations ... subunit mutations are both sensitive and resistant to volatile anesthetics. Anesth Analges 95, 578–582. 38 Takasaki M, Tatara T, Suezaki Y, Shirahama K, Kamaya H, Ueda I & Totoki...

Ngày tải lên: 19/02/2014, 18:20

... advance, in an adaptor grammar the subtrees, as well as their probabilities, are learnt from the train- ing data. In order to make parsing and inference tractable we require the leaves of these ... f-score considerably. 3.6 Collocation syllable adaptor grammar The collocation syllable adaptor grammar is the same as the unigram syllable adaptor grammar of Figure 7, except tha...

Ngày tải lên: 20/02/2014, 09:20

... Orientale, Alessandria, Italy; 3 Dipartimento di Biochimica e Biologia Molecolare, Universita ` di Parma, Italy This paper reports the isolation and characterization of the regulatory moiety of the ... hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component Ersilia Griva 1 , Enrica Pessione 1 , Sara Divari 1 , Francesc...

Ngày tải lên: 21/02/2014, 00:20

... one-to-one/one-to-many/many-to-many relationships, set theory, boolean algebra, or predicate calculus and the proper no~atlon may he of great assis- tance in formulating queries. Mathematicians (and musicians, ... terminal operators prefer natural language because they are already familiar with it, and that it gives the terminal operator the great- est power and flexibil...

Ngày tải lên: 21/02/2014, 20:20

... efficient, reasonably priced and safe antimalarials has called for the search for new drug targets and ultimately new drugs. Because heli- cases contain multiple functional domains and a vari- ety of enzymatic ... PfDH45 is a homolog of eukaryotic initiation factor 4A contains helicase and ssDNA- dependent ATPase activities and is expressed in all the development...

Ngày tải lên: 18/02/2014, 16:20