Tài liệu Báo cáo khoa học: Regulation of dCTP deaminase from Escherichia coli by nonallosteric dTTP binding to an inactive form of the enzyme ppt

... of dCTP deaminase. Mutational analysis of amino acid residues involved in dTTP regulation of dCTP deaminase The design of the mutant enzymes H121A and V122G was inspired by the results from analysis ... in particular in dTTP synthesis, both the dCMP- and the dCTP deaminases are inhibited by dTTP [5,12]. For dCMP deaminase, dTTP regulation occurs...

Ngày tải lên: 18/02/2014, 16:20

... growth in the trypto ne–yeast extract medium E. coli cells formed only insignificant amounts of glycogen irrespective of a proceeding transformation with the GLG 2 gene or an induction of the transgene ... revealed by high performance anion exchange chromatography, the intracellular maltooligosaccharide pattern of the b acterial cells expressing the functional eukaryo...

Ngày tải lên: 19/02/2014, 16:20

... membrane with the release of cytochrome c and the formation of the apoptosome [38]. The major pro-apoptotic protein Bax exists mainly in the cytosol or loosely attaches to mitochondria in an inactive form. ... dissociation from translocating to the mitochondria by a conformation change, and 14-3-3 binding leads to protection of Bad phosphorylation at Ser112,...

Ngày tải lên: 14/02/2014, 22:20

... factors are involved in the regulation of life span and progression of cancer and they are also important for developmental processes such as gametogenesis, neurogenesis and mainte- nance of ... Hsp70i gene during mitosis and to enhance the DNA -binding capacity of HSF2 [96,98]. However, another report showed that the modification rather hinders the DNA- binding activity...

Ngày tải lên: 18/02/2014, 04:20

... hindered by the occlud- ing loop and occurs by a two-step mechanism in which the N-terminus of the inhibitor first binds to the enzyme, displacing the occluding loop, followed by the binding of another ... active site to which 2A2 mAb binds, to determine the interference with the binding of substrates and other inhibitors and to iden- tify the mechani...

Ngày tải lên: 18/02/2014, 11:20

... termination of the pr otein s ynthesis. These data a dd to the growing body of information supporting a preponderant role of p38 in the regulation of gene expression at the level of mRNA stability. The ... amounts of e vidence support a n obligatory role for the actin cytoskeleton in the regulation of specific genes by small GTPase proteins. The m orpholog...

Ngày tải lên: 19/02/2014, 16:20

... [51]. The most significant result is that the average velocity was increased by the addition of T800, from 2.5 to 3.4 lmÆs )1 and from 3.9 to 4.3 lmÆs )1 in the absence and the p resence of Tm–Tn, respectively ... in the same way as T800 [64,69]. Inhibition of the ATPase activity was then explained by a slowing down of the formation of active complex in so...

Ngày tải lên: 19/02/2014, 16:20

... according to the manufacturer’s instruction. Statistics The data are presented as means ± standard errors of the mean (SEMs), and were statistically analyzed by means of the unpaired t-test or the ... University of Pharmaceutical Sciences, Japan Introduction The amount of adipose tissue in the body is an impor- tant factor in the maintenance of energy balance,...

Ngày tải lên: 16/02/2014, 09:20

... obtained from densitometric tracing of the autoradiograms; for each target site, the intensity of the R 53 band resulting from p53 binding to unmodified DNA was taken as 1.0, and the intensities of bands ... cisplatin IACs within the p53DBSs due to the occurrence of the GG, AG and ⁄ or GNG motifs, the stronger the inhibition of the p53 sequence-specific D...

Ngày tải lên: 19/02/2014, 05:20

... structure and con- formational transitions of chromatin. The basic repeating unit of chromatin, the nucleo- some, represents the first level of the chromatin organi- zation [6]. The major part of the ... length, the bead in the trap will start to displace from the center of the trap and the force which is trying to bring the bead back is linearly pro- po...

Ngày tải lên: 14/02/2014, 18:20