Tài liệu Báo cáo khoa học: Regulation of dCTP deaminase from Escherichia coli by nonallosteric dTTP binding to an inactive form of the enzyme ppt
Tài liệu Báo cáo khoa học: Regulation of dCTP deaminase from Escherichia coli by nonallosteric dTTP binding to an inactive form of the enzyme ppt
... of dCTP deaminase. Mutational analysis of amino acid residuesinvolved in dTTP regulation of dCTP deaminase The design of the mutant enzymes H121A and V122Gwas inspired by the results from analysis ... in particular in dTTP synthesis,both the dCMP- and the dCTP deaminases areinhibited by dTTP [5,12]. For dCMP deaminase, dTTP regulation occurs by binding of the inhibitor to an allosteric site ... between the dCTP( dUTP) -binding conformer and the dTTP- binding conformer of dCTP deaminase. We were not able to identify structuralchanges in the main chain of the subunit, or in the interaction of...
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Tài liệu Báo cáo khoa học: Yeast glycogenin (Glg2p) produced in Escherichia coli is simultaneously glucosylated at two vicinal tyrosine residues but results in a reduced bacterial glycogen accumulation docx
... growth in the trypto ne–yeast extract mediumE. coli cells formed only insignificant amounts of glycogenirrespective of a proceeding transformation with the GLG 2gene or an induction of the transgene ... revealed by high performance anion exchange chromatography, the intracellular maltooligosaccharide pattern of the b acterialcells expressing the functional eukaryotic transgene wassignificantly ... biosynthesis was, to someextent, restored and the g lycogen content of the comple-mented cells was a pproximately 10% of that of the wild-typecontrol. A complete loss of the in vivo function of...
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Tài liệu Báo cáo khoa học: Regulation of DNA fragmentation: the role of caspases and phosphorylation doc
... membranewith the release of cytochrome c and the formation of the apoptosome [38]. The major pro-apoptotic proteinBax exists mainly in the cytosol or loosely attaches to mitochondria in an inactive form. ... dissociation from translocating to the mitochondria by a conformationchange, and 14-3-3 binding leads to protection of Badphosphorylation at Ser112, Ser136 and Ser155 [86]. The dissociation of Bax and ... dephosphor-ylation of both Ser112 and Ser136, Bad is dephospho-rylated at Ser155, which allows translocation to mitochondria and the binding of Bcl-xL [86], andincreases the release of cytochrome c from mitochon-dria...
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Tài liệu Báo cáo khoa học: Regulation of the members of the mammalian heat shock factor family doc
... factors are involved in the regulation of life span and progression of cancer and they are also important fordevelopmental processes such as gametogenesis, neurogenesis and mainte-nance of ... Hsp70igene during mitosis and to enhance the DNA -binding capacity of HSF2 [96,98]. However, another reportshowed that the modification rather hinders the DNA- binding activity of HSF2, without interfering ... furtherinvestigations. In accordance with the fundamentalrole of HSF1 in the heat shock response, the require-ment of HSF1 and HSF4 in development of the lensand olfactory epithelium is limited to the postnatalperiod,...
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Tài liệu Báo cáo khoa học: Regulation of cathepsin B activity by 2A2 monoclonal antibody docx
... hindered by the occlud-ing loop and occurs by a two-step mechanism in which the N-terminus of the inhibitor first binds to the enzyme, displacing the occluding loop, followed by the binding of another ... active site to which 2A2mAb binds, to determine the interference with the binding of substrates and other inhibitors and to iden-tify the mechanism by which it regulates the activity of cathepsin ... cathepsin B is able to participate in both the early and late stages of protein breakdown. The activity of cathepsin B is regulated in manyways, ranging from the pH of the environment to the presence...
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Tài liệu Báo cáo khoa học: Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics docx
... termination of the pr otein s ynthesis.These data a dd to the growing body of informationsupporting a preponderant role of p38 in the regulation of gene expression at the level of mRNA stability. The ... amounts of e vidence support a n obligatoryrole for the actin cytoskeleton in the regulation of specificgenes by small GTPase proteins. The m orphology of the actin cytoskeleton upon treatment of ... reported to release labile transcripts s uch a s those of TNF-a and Cox-2 from a state of translational arrestimposed by AREs within the 3¢-UTR by regulatingdeadenylation rather t han d ecay of the...
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Tài liệu Báo cáo khoa học: Regulation of the actin–myosin interaction by titin doc
... [51]. The most significantresult is that the average velocity was increased by the addition of T800, from 2.5 to 3.4 lmÆs)1and from 3.9 to 4.3 lmÆs)1in the absence and the p resence of Tm–Tn,respectively ... in the same way as T800 [64,69]. Inhibition of the ATPase activity was then explained by a slowingdown of the formation of active complex in solution. On the other hand, the acceleration of the ... traceobtained from the analysis of the movement of a single filament during the in vitro motility assay. (B and C) Box representation of the velo-cities (B) and the percentile of STOPS (C) obtained...
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Tài liệu Báo cáo khoa học: Pronounced adipogenesis and increased insulin sensitivity caused by overproduction of prostaglandin D2 in vivo pptx
... according to the manufacturer’s instruction.Statistics The data are presented as means ± standard errors of the mean (SEMs), and were statistically analyzed by means of the unpaired t-test or the ... University of Pharmaceutical Sciences, JapanIntroduction The amount of adipose tissue in the body is an impor-tant factor in the maintenance of energy balance,through its ability to store and release ... determined from an image at the level of the umbilicus. Subcutaneous fat wasdefined as the extraperitoneal fat between skin and muscle. The intraperitoneal part with the same density as the sub-cutaneous...
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Tài liệu Báo cáo khoa học: DNA modification with cisplatin affects sequence-specific DNA binding of p53 and p73 proteins in a target site-dependent manner pptx
... obtained from densitometric tracing of the autoradiograms; for each targetsite, the intensity of the R53band resulting from p53 binding to unmodified DNA was taken as 1.0, and the intensities of bands ... cisplatinIACs within the p53DBSs due to the occurrence of the GG, AG and ⁄ or GNG motifs, the stronger the inhibition of the p53 sequence-specific DNA binding to these targets caused by the DNA treatment ... in the genome of living cells is naturally stochastic on the one hand, and influenced by the actual structuraland functional state of the given chromatin domainon the other. Moreover, some of the...
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Tài liệu Báo cáo khoa học: Chromatin under mechanical stress: from single 30 nm fibers to single nucleosomes pdf
... structure and con-formational transitions of chromatin. The basic repeating unit of chromatin, the nucleo-some, represents the first level of the chromatin organi-zation [6]. The major part of the ... length, the bead in the trap will start to displace from the center of the trapand the force which is trying to bring the bead back is linearly pro-portional to this displacement. Thus, the change ... correspond to an assembly of individualnucleosomes, but rather to formation and release of rather long ‘loops’ (200–400 nm). The fact that the energy provided by the added ATP in the system...
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