Tài liệu Báo cáo khoa học: Evolutionary divergence of valosin-containing protein ⁄cell division cycle protein 48 binding interactions among endoplasmic reticulum-associated degradation proteins ppt

... FEBS Evolutionary divergence of valosin-containing protein ⁄ cell division cycle protein 48 binding interactions among endoplasmic reticulum-associated degradation proteins Giacomo Morreale, Laura Conforti, ... For example, valosin-containing protein (VCP) and its yeast homologue cell division cycle protein 48 (Cdc48p), which help to direct polyubiq...

Ngày tải lên: 18/02/2014, 13:20

... excluding large proteins. The mechanism of preventing the digestion of bigger proteins was recently clarified when the 3D structure of POP was solved [4]. The enzyme consists of a peptidase and ... from the rest of the POP family members that are cytoplasmic proteins [3]. However, a membrane bound form of POP has also been characterized from bovine brain but the sequence of...

Ngày tải lên: 19/02/2014, 13:20

... stable recombinant protein in E. coli at up to  4% of the total soluble protein content of the cell [48] . Fig. 3. Microbial mechanisms of glyphosate degradation. (A) Two principal pathways of glyphosate degradation ... however, was devoid of substrate or inhibitor, and consequently did not reveal the nature of the active site or the mode of action of glyphosate. A dec...

Ngày tải lên: 14/02/2014, 14:20

... importance of carbohydrate- aromatic interactions for the molecular recognition of oligosaccharides by proteins: NMR studies of the struc- ture and binding affinity of AcAMP2-like peptides with non-natural ... of proteins, nucleic-acids, and organic molecules. J Am Chem Soc 117, 5179–5197. 39 Ryckaert JP, Ciccotti G & Berendsen HJC (1977) Numerical-integration of cartesia...

Ngày tải lên: 18/02/2014, 17:20

... sperm protein, enterokinase and agrin) modules, minimally comprised of 120 amino acids of which 60% show strong conservation between proteins, are usually found in extracellular domains of dimeric ... highly conserved among the SEA modules of MUC1 glycoproteins from different species, and is found in other membrane-tethered mucins including MUC3, MUC12, MUC16 and MUC17 and other...

Ngày tải lên: 19/02/2014, 18:20

... models of AD [8], suggesting that disruption of the association of these proteins could represent a poten- tial therapeutic strategy for the treatment of AD. Here, we used GST fusion proteins of ... status of tau [21]. Fyn also interacts with proteins through its SH2 domain, which recognizes phosphorylated tyrosines on target proteins [22,23]. Such interactions regulate...

Ngày tải lên: 14/02/2014, 14:20

... diffusion method. Two microlitres of protein solution at a concentration of 9 mgÆmL )1 and 2 lL of crystallization solution were equilibrated against 500 lL of reservoir solution. Initial screens ... significance of transient protein protein interactions. J Mol Biol 325, 991–1018. 54 Kiiskinen L-L, Kruus K, Bailey M, Ylosmaki E, Siika- aho M & Saloheimo M (2004) Expression...

Ngày tải lên: 14/02/2014, 18:20

... cargo binding sites, referred to as the major and minor binding sites [1]. These sites are located in the concave face of the protein near regions of invariant Trp and Asn arrays. The major binding site ... reservoir consisted of 500 lL of 0.7 m sodium citrate, 10 mm dithiothreitol and 70 mm Hepes at pH 7.4. Drops consisted of 0.75 l L of importin-a (70-529) at 18 mgÆmL )1...

Ngày tải lên: 14/02/2014, 19:20

... comparison. Effect of geographical variation in dengue virus on the performance of the primer cocktail Each of the four serotypes of dengue virus can be classi- fied into several genotypes, defined as a group of ... analysis of the structure and function of West Nile non-structural proteins, in Biotechnology and Bioinformatics Symposium 2008. 24 Misra M & Schein CH (2007) Fla...

Ngày tải lên: 14/02/2014, 19:20

... (Fig. 3A). Three of the ligands, OD2 of Asp165, NE2 of His79 and NE2 of His169, formed an equatorial plane. The other protein ligand, NE2 of His31, bound to the metal, in the company of a water oxygen, ... conformation around the active site of ApeSOD is independent of the presence of a metal cofactor. Figure 3D shows the superimposition of the active site structures of...

Ngày tải lên: 14/02/2014, 22:20