... ª 2009 FEBS Simplified yet highly accurate enzyme kinetics for cases of low substrate concentrations Hanna M. Ha¨rdin1,2, Antonios Zagaris2,3, Klaas Krab1and Hans V. Westerhoff1,4,51 ... with enzymes acting on polynucleotides (such asDNA gyrase and ribosomes), where the concentrations of enzymes and binding sites are often of the sameorder of magnitude. In all of these cases, ... 3.6141516171920HPr P EIIAFig. 8. The analogue of Fig. 7 for x ¼x ¼ [EIICBÆPÆGlc]. Three of the eight plots are shown. Enzyme kinetics for low substrate concentrations H. M. Ha¨rdin et al.5502...