Tài liệu Báo cáo khoa học: Golgi reassembly stacking protein 55 interacts with membrane-type (MT) 1-matrix metalloprotease (MMP) and furin and plays a role in the activation of the MT1-MMP zymogen pdf

... stacking protein 55 interacts with membrane-type (MT) 1-matrix metalloprotease (MMP) and furin and plays a role in the activation of the MT1-MMP zymogen Christian Roghi 1,2 , Louise Jones 2 *, Matthew ... general mechanism for furin- mediated activation of transmem- brane substrates. The activation of ADAM17 by furin [68] and ou...

Ngày tải lên: 18/02/2014, 04:20

... ribosome attains its high level of accuracy in translation, its catalytic triad (rRNA, ribosomal protein, and the peptidyl-tRNA substrate), and the mode of action of many antibiotics, enabling the design ... effort in preparing and analyzing the full-length protein can be extremely rewarding, as shown by the information obtained with the tumor suppressor prot...

Ngày tải lên: 14/02/2014, 19:20

... control. The target mRNA was normalized against actin in the same sample. The PCR primers were as follows: actin forward, 5¢-GA AATCGTGCGTGACATCAAAG-3¢; actin reverse, 5¢-TG TAGTTTCATGGA TGCCACAG-3¢; ... the protein degradation, destabilizing the Akt-1 protein. In addition, the AMP-activated protein kinase pathway can affect Akt-1 protein levels in the cell [39]. It...

Ngày tải lên: 16/02/2014, 09:20

... [12,13,17]. YpkA-mediated phosphorylation of Gaq impairs guanine nucleotide binding and subsequently inhibits Gaq-mediated signalling pathways including RhoA activation and cytoskeletal rearrangements in the ... Immunoprecipitated material was analysed by SDS ⁄ PAGE and silver staining, and the large band corresponding to the expected molecular mass of OTUB1 as well as...

Ngày tải lên: 16/02/2014, 15:20

... TLR contain two major domains: the extracellular ligand-binding domain, characterized by leucine-rich repeats and the intracellular Toll ⁄ IL-1 receptor domain (TIR domain) [3]. In mammals, 13 TLR ... H & Miyamoto E (1999) Mitogen-activated protein kinase activation and regulation of cyclooxygenase 2 expression by platelet-activating factor and hCG in human endometrial...

Ngày tải lên: 18/02/2014, 13:20

... the catalytic dyad (Cys186 and His144) and the residues anchoring the aspartate residue in the P1 position (Arg87, Ala145 and Gln184) which are in the p20 chain. The inhibitors bind the two active ... part by the Georgia State University Molecular Basis of Disease Program, the Georgia Research Alliance, and the Georgia Cancer Coalition. We thank the staff a...

Ngày tải lên: 18/02/2014, 16:20

... elongated protein of 947 amino acids and containing a highly hydrophobic domain rich in cysteine [9] (Fig. S1). An extensive search in databases failed to reveal significant homology of the additional ... 1C). The 130 kDa isoform of the K650M mutant was only weakly and variably detected in immunoblots. Scanning densitometry of the gel fur- ther indicated that the...

Ngày tải lên: 19/02/2014, 00:20

... the nature of the LDL modification; these were quantified at the 96 h time point (Fig. 2D). The extent of protein endocytosis, degradation and intracellular accumulation of apoB was increased in HMDMs ... and glycated apoB in the cells. The rate of removal of the latter species is slow, with only 20–30% of the glycated protein being degraded over a 24...

Ngày tải lên: 19/02/2014, 02:20

... specifying cGMP over cAMP as a ligand [12]. Taken together the b1–b3 region of the tandem GAF domain appears to be a major determinant of purine selection. The tandem GAF domain of the Anabaena AC ... compares the sequences of the cAMP- binding GAF tandem domain of PDE10, the PDE from Trypanosoma brucei [15] and cyaB1 and cyaB2 ACs with the cGMP-binding tan...

Ngày tải lên: 19/02/2014, 02:20

... Evidence of insulin-stimulated phosphorylation and activation of the mammalian target of rapamycin mediated by a protein kinase B signaling pathway. Proc Natl Acad Sci USA 95, 7772–7777. 12 Nave BT, ... 5¢-CGAGCCAGCAAGACGGATGA G-3¢ (sense) and 5¢-GGCTCTACAAGCTTACTCACTAC CCGG-3¢ (antisense) were used, and the amplified products were normalized by loading an equal amount o...

Ngày tải lên: 19/02/2014, 06:20