Tài liệu Báo cáo khoa học: Degradation of tropoelastin by matrix metalloproteinases – cleavage site specificities and release of matrikines pptx

... compilation ª 2010 FEBS Degradation of tropoelastin by matrix metalloproteinases – cleavage site specificities and release of matrikines Andrea Heinz 1 , Michael C. Jung 1 , Laurent Duca 2 , Wolfgang ... solvent-accessi- ble and hence restrict cutting in this area. Cleavage site specificity To gain a better understanding of the cleavage site specificiti...

Ngày tải lên: 16/02/2014, 14:20

... and GST- lofMPK6. However, when lofMPK3 and lofMPK6 proteins were tested for phosphorylation with OXI1, no phosphorylation of lofMPK3 or lofMPK6 was observed (Fig. 5A). On the other hand, when ... characterized by a conserved DFD amino acid motif in subdomain VII of the catalytic domain and by the presence of an amino acid insertion of variable size between subdomains VII a...

Ngày tải lên: 14/02/2014, 19:20

... the surge of 8K-BLP release in this period is reminiscent of the massive release of IGF-I and IGF-II during pubertal and fetal development, respectively, in mam- mals [4]. Finally, like that of IGFs, ... essential roles in fetal and postnatal growth and development of mammals. They are secreted by a wide variety of tissues, with the liver being the major source of...

Ngày tải lên: 18/02/2014, 13:20

... by aminoacylation and editing? Trends Genet 12, 60 4–6 10. 3 Pauling L (1958) The probability of errors in the pro- cess of synthesis of protein molecules. In Festschrift Arthur Stoll, pp. 59 7–6 02. ... misacylation and transla- tional recoding of lysine. Plant J 50, 62 7–6 36. 43 Rosenthal GA (1977) The biological effects and mode of action of l-canavanine, a struc...

Ngày tải lên: 18/02/2014, 13:20

... was four-fold to five-fold higher in the presence of 100 and –2 288 –2 152 –2 000 –1 788 –1 260 –3 000 – 2 3 4 E R E 6 E R E 5 E R E 4 E R E 3 E R E 2 E R E 1 + 1 – 2 3 4 E R E 6 E R E 5 E R E 4 E R E 3 E R E 2 E R E 1 + 1 Fig. ... ERE2, ERE3 and ERE4 of the HOXC13 promoter. As seen in Fig. 5A, no significant binding of actin was observed in any of the EREs...

Ngày tải lên: 18/02/2014, 14:20

... determined at pH 5 and 0.05% SDS, and DO activity at pH 7 and 0.02% SDS. Fig. 3. Dependence of the TH activity of extracts of R3 and the two mutant strains on the presence of cofactor, L-dopa. ... independent of the addition of l-dopa cofactor, and the lag period before reaching the maximal reaction rate without this addition is short ( 4 0–6 0 s under standard condit...

Ngày tải lên: 19/02/2014, 07:20

... Murakami 2 and Kenji Aoki 2 1 Division of Science of Biological Resources, Graduate School of Science and Technology, 2 Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, ... nm. The cofactor from the purified enzyme was detected by fluorescence ( F-1050) at an excitation and emission wavelength of 450 and 530 nm, respectively. Ammonia release...

Ngày tải lên: 19/02/2014, 16:20

... occur. Acknowledgements This work was supported by a grant from Ministry of Science and Technology of the Spanish Government (SAF 200 2–0 2785), INTAS grant (9 7–1 504), and the Netherlands’ Organization for Scientific Research. ... Netherlands; 3 Department of Biochemistry and Molecular Biology, Faculty of Chemistry and CeRQT at Barcelona Scientific Parc, University of...

Ngày tải lên: 19/02/2014, 16:20

... curves and gave linear double-reciprocal plots in the range 1–1 0 mm pyruvate, 5–6 0 mm methyl- amine, and 0.0 5–0 .3 mm NADPH. The families of lines obtained from the double-reciprocal plots of reac- tion ... dehydrogenases and opine dehydrogenases and has no sequence motif char- acteristic of the Rossmann fold, indicating that the structure of the nicotinamide nucleoti...

Ngày tải lên: 19/02/2014, 16:20

... outer and middle rings of transmembrane domains. Curr Opin Drug Discov Devel 6, 62 0–6 32. 20 Miller KW (2002) The nature of sites of general anaes- thetic action. Br J Anaesth 89, 1 7–3 1. 21 Sandermann ... Induction of lipid–protein mis- match by xenobiotics with general membrane targets. Biochim Biophys Acta 1150, 13 0–1 33. 22 Sandermann H & Pflugmacher D (1996) Induc...

Ngày tải lên: 19/02/2014, 18:20