Tài liệu Báo cáo khoa học:Tyrosine phosphorylation of tau regulates its interactions with Fyn SH2 domains, but not SH3 domains, altering the cellular localization of tau ppt

... compilation ª 2011 FEBS 2937Tyrosine phosphorylation of tau regulates its interactions with Fyn SH2 domains, but not SH3 domains, altering the cellular localization of tau Alessia Usardi1, Amy M. ... status of tau also affects the binding of tau to Fyn- SH3, CHO cells werecotransfected with Fyn together with either wild-typeor the YF mutant forms of tau. Cell lysates containingequal amounts of ... thattyrosine phosphorylation mediates the association of tau with Fyn- SH2, but not with Fyn- SH3. This dem-onstrates that different molecular mechanisms exist forthese two distinct interactions of Fyn...

Tài liệu Báo cáo khoa học: Major phosphorylation of SF1 on adjacent Ser-Pro motifs enhances interaction with U2AF65 doc

... suggesting that phosphorylation of these sites might regulate the formation of the SF1-U2AF-RNA complex. We ﬁrst tested the effect of phosphorylation on the interaction of SF1 with U2AF65by ... PAGE. (B) The ability of similar amounts of the indicatedforms of KIS to phosphorylate SF1f in vitro was compared, show-ing that deletion of or within the UHM domain of KIS impaired SF1f phosphorylation. ... control-ling the phosphorylation state of SF1. Finally, SF1 phosphorylation on its SPSP motif enhanced the inter-action of SF1 with U2AF65, and also promoted forma-tion of a ternary complex with...

Tài liệu Báo cáo khoa học: Single phosphorylation of Tyr304 in the cytoplasmic tail of ephrin B2 confers high-afﬁnity and bifunctional binding to both the SH2 domain of Grb4 and the PDZ domain of the PDZ-RGS3 protein ppt

... the Grb4 SH2 domain. The afﬁnity of the binding interactions was evaluated by measuring the changes of ﬂuorescence polarization of the peptides ateach step of titration with the Grb4 SH2 domain, ... Bioscience). The sample of the Ni-nitiloacetic acid agarose beads with the bound SH2 protein at pH 5.9 was not shown here as the protein was already pure. The position of the SH2 protein is marked by the ... to the Grb4 SH2 domain. Therefore, the high-afﬁnity Grb4 SH2 binding of the ephrinB2(301–333) fragment conferred by the Tyr304 phosphorylation isindependent of phosphorylations at the other...

Tài liệu Báo cáo khoa học: Structural characterization of Ca2+/CaM in complex with the phosphorylase kinase PhK5 peptide pdf

... againstFig. 4. (Top) Overlay of two spectra from the titration of Ca2+⁄ CaM with PhK5 peptide. The red spectrum shows the position of peaksprior to the addition of PhK5 peptide. The blue spectrum shows ... ratio of Ca2+⁄ CaM to PhK5had been reached. The inset shows an expanded view of the indi-cated area of the spectrum. (Bottom) An overlay of spectra from the titration of Ca2+⁄ CaM with PhK13 ... typical traits associated with such anautoinhibitory mechanism. The sequence of the PhKcsubunit encodes a C-terminal extension to the proteinkinase domain and treatment of the kinase with Keywordscalmodulin;...

Tài liệu Báo cáo khoa học: NMR structure of AII in solution compared with the X-ray structure of AII bound to the mAb Fab131 pptx

... average co-ordinates (the blue colour denotes the side chains of Arg2, Tyr4, Ile5, His6 and the yellow the side chains of Val3and Pro7) (a) with the the X-ray structure of AII in the Fab131–AII complex ... alignment of the fragment 4–7 of the 13 ensemble structures of AII to the fragment 3–6 of the X-ray structure of AII.Fig. 6. Structure of a representative folded conformer of AII showing the van ... structure of the Tyr4-Ile5-His6-Pro7sequence, which is the most immunogenic epitope of the peptide, with the Asp1 side chain oriented towards the interior of the turn approaching the C-terminus;...

Tài liệu Báo cáo khoa học: Molecular basis of glyphosate resistance – different approaches through protein engineering doc

... inresidues within the perimeter of the active site; posi-tions 31, 132 and 135 belong to loop 20 or loop 130. Of note is the fact that all four substitutions in the active site reduce the size of the ... glyphosate resistance traits is the reduction in cost brought about by the entry of genericproducers following the expiration of the patent on the molecule itself in 2000.There are two basic strategies ... under-standing of the molecular mode of action of glypho-sate and the molecular basis for glyphosate resistance.Structure and function of EPSPSEPSPS catalyzes the transfer of the enolpyruvyl moietyof...

Tài liệu Báo cáo khoa học: Crystal structure of an ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases ppt

... [22–24]. The other phylum of the fungi, the Ascomycota or sac fungi, is much less studied,and only the crystal structure of Melanocarpus albomy-ces laccase (MaL) has been solved [25]. The fold of the ... Structural formulas of the substrates are presented. Redox potentials (E°)ofT1coppers of the laccases and redox potentials of the substrates at pH 4.5 and pH 6.0 are provided, together with the redox ... redox potential(E°) of the substrate must be low enough, as the rate of the reaction has been shown to depend on the dif-ference between the redox potentials of the enzymeand the substrate (DE...

Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt

... signiﬁcant interactions with importin-a.This primarily occurs by the formation of a hydrogenbond between the side-chain hydroxyl group of Tyr205and the importin-a main chain at the C-terminus of the ... chain with well-deﬁned carbonyl and amidebackbone groups. The one exception to this is the location of the amide group of Lys204 at P3, wherethere is a break in the main-chain density at the ... competes for the importin-a binding site,reducing binding afﬁnity for cargo proteins and help-ing to facilitate the release of the cargo within the nucleus [10,11]. The removal of the autoinhibitorydomain...

Tài liệu Báo cáo khoa học: PCR detection of nearly any dengue virus strain using a highly sensitive primer ‘cocktail’ ppt

... in the row associated with that serotype covered by the primers of the group associated with that column; the primers are categorized according to the strainsthat they detect. The number of ... [25]. The R2value is also reported.Reverse e-PCR testing of the primer cocktail with 1688 strains of dengue virusTo predict the sensitivity of the primer cocktail to diversestrains of the ... by the primer pair 1G5P30 with perfectprimer match. The genotype classiﬁcation of each strain was determinedeither by referring to the published literature [28–34] or byusing the Dengue Genotype...

Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

... [26]. These ﬁndingslead to the hypothesis that Fe-bound ApeSOD mimics the product-inhibited form and the shift of Tyr39 sup-presses the release of the peroxide product. This maybe one of the ... coordination with six ligands. The additional water occupied the postulated superoxide bind-ing site. The thermal stability of the enzyme was compared with superoxidedismutase from Thermus thermophilus, ... solution. Figure 1C illustrates the superimpo-sition of ApeSOD with the cambialistic SOD fromP. shermanii. The overall structures of these enzymeswere similar; the rmsd of the 747 Ca atoms was0.695...

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