Tài liệu HPLC for Pharmaceutical Scientists 2007 (Part 21) ppt

... years. Today, preparative HPLC has also become a powerful technology in phar- maceutical development and production either for isolation of impurities, for 937 HPLC for Pharmaceutical Scientists, Edited ... 0.003–0.1 semipreparative for toxicology Semipreparative 10–40 Intermediates for 25–100 0.1–5 —preparative lab synthesis Pilot plant 100–300 Manufacturing of 100–1000 20–5...

Ngày tải lên: 26/01/2014, 19:20

... ions on the basis of mass-to-charge ratios, 281 HPLC for Pharmaceutical Scientists, Edited by Yuri Kazakevich and Rosario LoBrutto Copyright © 2007 by John Wiley & Sons, Inc. low-polarity ... the most widely used systems for analysis of large bio- molecules. For a continuous ion source, the ions can be stored for a short period of time and pulsed out for analysis. In the...

Ngày tải lên: 15/12/2013, 12:15

... metabolites are important biotransformation pathways for many drugs and xenobiotics [127, 132, 133]. For example, the formation of acyl glucuronide conjugate forced the withdrawal of four marketed ... intermediates for formation of the keto acid, were also observed in the radiochromatogram of rat plasma (spectrum not shown) [131]. PRACTICAL APPLICATIONS 335 Scheme 11. Mechanism for th...

Ngày tải lên: 15/12/2013, 12:15

... development; these include speed 347 HPLC for Pharmaceutical Scientists, Edited by Yuri Kazakevich and Rosario LoBrutto Copyright © 2007 by John Wiley & Sons, Inc. for these studies. Fast gradient ... the ACD prediction of the solubil- ity for the ionized form of bases and acids shows greater deviation than for the solubility predicted for the neutral form of the molecule...

Ngày tải lên: 15/12/2013, 12:15

... separa- tion efficiency in HPLC is more related to instrument optimization, column 25 HPLC for Pharmaceutical Scientists, Edited by Yuri Kazakevich and Rosario LoBrutto Copyright © 2007 by John Wiley ... reso- lution has been for long a subject of extensive theoretical studies [4–6], with the goal to express resolution as a function of k, a, and N. Unfortunately, the direct algebr...

Ngày tải lên: 24/12/2013, 18:15

... 3. 139 HPLC for Pharmaceutical Scientists, Edited by Yuri Kazakevich and Rosario LoBrutto Copyright © 2007 by John Wiley & Sons, Inc. organic solvent increases the solvent strength and allows for ... extends both retention and selectivity in HPLC [high-performance liquid chromatography] over an extremely broad range.” They compared gas chromatography with HPLC, citing “in...

Ngày tải lên: 24/12/2013, 18:15

... based on the forced transport of the polymer molecules through the porous stationary-phase media under the conditions of suppressed interactions of the 263 HPLC for Pharmaceutical Scientists, ... in reversed-phase chromatography applications for example, if the area% does not agree with the assay% value for pharmaceutical analysis of a particular active pharmaceutical ingredient...

Ngày tải lên: 24/12/2013, 18:15

... validated methods. 455 HPLC for Pharmaceutical Scientists, Edited by Yuri Kazakevich and Rosario LoBrutto Copyright © 2007 by John Wiley & Sons, Inc. 9.4.1 Accuracy The test for accuracy is intended ... support for qualifying the pro- posed LOQ as the official LOQ for the method. 9.4.3.1 Linearity Example (Assay and Content Uniformity). An example for linearity for Assay...

Ngày tải lên: 24/12/2013, 18:15

... addition, forced decomposition studies must also be performed for dif- ferent dosage forms (capsule, tablet, suspension, injectable, etc.) of the same drug substance. 688 ROLE OF HPLC DURING FORMULATION ... (capsule dosage form) of the development compound A with aspirin was proposed. The preformulation TEST METHODS FOR MOST COMMON DOSAGE FORMS 689 Figure 15-2. HPLC chromatogram obtai...

Ngày tải lên: 22/01/2014, 00:20

... material. In that sense, data generated using HPLC and hyphenated HPLC techniques such as HPLC/ MS, HPLC- MS/MS, and HPLC/ NMR serve as the foundation for declaring the existence of reproducible ... [7] guidelines for method validation, the existence of a final synthetic process for the active pharmaceutical ingredients (APIs), and final market image of the pharmaceutical dosa...

Ngày tải lên: 22/01/2014, 00:20