tt01a i a genomic variant isolated from the laboratory maintained nematode heterorhabditis bacteriophora
Báo cáo y học: "Plastic architecture of bacterial genome revealed by comparative genomics of Photorhabdus variants" ppsx
... following additional data files are available with this paper Additional data file is a figure showing the deletion in the lopT1 gene in TT01 /I strain and the six variants Additional data file is a figure ... variant isolated from the laboratory- maintained nematode Heterorhabditis bacteriophora The nematode Heterorhabditis bacteriophora TH01, harboring the TT01 wild-type strain, was collected in Trinidad ... Additionalisplasmidsshowing six andof I- CeuI-hydrolyzed genomic Click microarray therDNAthethisvariants and the/ Iin TT01αstudy microarray .the accordingvariants.deletion of variants in TT01α /I VAR*of...
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Tiểu luận tiếng anh : Robinson Crusoe – A Representative of the English Bourgeoisie in the early 18th century
... Crusoe and Friday It’s the relation of love and mutualism as Friday shared Crusoe in all the work and activities on the island There is also a very important relation that links the two man It is the ... the spiritual or the religious relationship It was Crusoe that teached Friday the rituals of the Christian doctrine, he was able to change him from cannibalism to a real Christian man who believed ... development The industrial revolution began: new machinery was invented that turned Britain into the first capitalist power of the world The capitalist path paved the way for heavier exploitation relations...
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báo cáo hóa học:" Phase I/II open-label study of the biologic effects of the interleukin-2 immunocytokine EMD 273063 (hu14.18-IL2) in patients with metastatic malignant melanoma" docx
... was tested in a phase I clinical trial aimed at evaluating its safety, toxicity and in vivo immunological effects in 33 patients with metastatic melanoma [7] This immunocytokine was given as a ... prior therapy Patients were to have at least melanoma lesions (other than a target lesion) available for outpatient biopsies The inclusion criteria initially required that the patients be HLA -A2 -positive ... immunoassay and drafted portions of the manuscript describing the assays WG participated in the design of the study and performed the statistical analysis AK participated in the study design and...
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Lời bài hát Rhythm Of The Rain
... từng biết đâm ch i nảy lộc Listen to the rhythm of the falling rain Telling me just what a fool I' ve been I wish that it would go and let me cry in vain And let me be alone again Hãy lắng ... i u tiếng m a r i Nó bảo t i kẻ khờ T i ước tạnh để khóc vô vọng Và bỏ l i n i cô đơn thêm lần Oh, listen to the falling rain Pitter pater, pitter pater Oh, oh, oh, listen to the falling rain ... oh, oh, listen to the falling rain Pitter pater, pitter pater Ô, lắng nghe nhịp i u tiếng m a r i Tí tách, tí tách Ô, ô, ô, lắng nghe nhịp i u tiếng m a r i Tí tách, tí tách ...
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Báo cáo y học: "Multivariate explanatory model for sporadic carcinoma of the colon in Dukes’ stages I and IIa"
... format for its later statistical analysis, and the quality controls were also made at this stage Statistical analysis An initial study was made on the set of records to obtain centralization and ... marker as predictors [20] Sample size was taken into account [21] A first analysis was made on the “raw” data package The selection of variables was always backward In the variables in which lost information ... that contain mucin or in the circulation, and that is located in the sialylated Lewis A blood group antigen [16-18] The individuals with the Lewis a- b genotype cannot synthesize this antigen (an...
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Tài liệu I MMIGRANT S MALL B USINESS OWNERS: A S IGNIFICANT AND G ROWING PART OF THE E CONOMY pdf
... Germany Portugal Brazil Hong Kong Ukraine Other USSR/Russia Japan China Vietnam Nigeria Colombia Thailand Nicaragua Peru Ecuador Trinidad and Tobago Guyana/British Guiana Jamaica Dominican Republic ... Venezuela Romania Nigeria Ireland Haiti Egypt/United Arab Rep Iraq Honduras Nicaragua South Africa Portugal Turkey France Thailand Trinidad and Tobago Guyana/British Guiana Syria Jordan All other ... Syria Iran Lebanon Jordan Italy Korea South Africa Ireland Iraq Pakistan Turkey Argentina Egypt/United Arab Rep Taiwan England Cuba Venezuela Canada United Kingdom, ns Romania Poland India France...
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Tài liệu Báo cáo khoa học: Post-translational modifications of the linker histone variants and their association with cell mechanisms docx
... H1 variants within a single species such as H sapiens, predominantly in the N-terminal and C-terminal tails, with, as stated, a conserved globular domain However, when similar H1 variants are ... such as azacitidine, decitabine, and zebularine, have been developed All three are cytidine derivatives that irreversibly inhibit DNMTs [11] As decitabine contains a deoxyribose group, it is incorporated ... incorporated into DNA [12] However, because azacitidine contains a ribose group, it is initially incorporated into RNA [12] Incorporation into DNA occurs when azacitidine is converted into 5-aza-2¢-deoxycytidine...
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Tài liệu Báo cáo khoa học: The localization of FGFR3 mutations causing thanatophoric dysplasia type I differentially affects phosphorylation, processing and ubiquitylation of the receptor pptx
... that propagate FGFR signals via different signalling pathways resulting in the regulation of many cellular processes including proliferation, differentiation, migration and survival [1–4] Dominant ... slightly increased by chloroquine treatment (Fig 6B), suggesting that the lysosomal pathway may also participate to their degradation The X807R mutant also exhibited an increased ubiquitylation ... mutants, the K650M mutant could elicit signalling via an alternative internal pathway involving c-Cbl and a Src-like kinase Taken together, the data reported here provide evidence that TDI is caused...
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Tài liệu Báo cáo khoa học: Quantitative analysis of the experimental O–J–I–P chlorophyll fluorescence induction kinetics Apparent activation energy and origin of each kinetic step Steve Boisvert, David Joly and Robert Carpentier doc
... Previous work done using qualitative or semiquantitative analysis of experimental FI traces from thylakoid membranes provided limited information In particular, the characteristics of the JI phase ... constant Our results indicate a different bioenergetic origin for each kinetic step of the FI rise, as the steps have different apparent EA values, with EA OJ < EA JI < EA IP In addition, we clearly ... its amplitude was only slightly decreased This observation is easily explained by the fact that a nonsaturating concentration of DCMU was used, meaning that only a fraction of the PSII reaction...
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Tài liệu Báo cáo khoa học: Unusual metal specificity and structure of the group I ribozyme fromChlamydomonas reinhardtii23S rRNA pptx
... [34] Discussion Kinetic analysis of Mn2+ inhibition The group I ribozyme literature indicates that divalent metals are required for tertiary folding and catalysis, and that Mg2+ or Mn2+ can satisfy ... folding of the ribozyme in Mn2+ is that binding of the cosubstrate to its site induces a conformational change in the RNA that inhibits Mn2+ binding at another inhibitory site(s) It may be relevant ... P4 and P6 [3,3537], and the relatively weak overall protection of the P4P5P6 domain (Fig 5C), indicate that this domain is not tightly packed against the catalytic domain Analysis of the predicted...
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Tài liệu Báo cáo khoa học: The role of the ESSS protein in the assembly of a functional and stable mammalian mitochondrial complex I (NADH-ubiquinone oxidoreductase) pptx
... other antibodies were as follows: anti-porin from Calbiochem, anti-HA from Covance BabCo, anti-mouse and anti-rabbit secondary antibodies from Bio-Rad Laboratories and Amersham Pharmacia Biotech, ... essential subunit for assembly of an active mammalian mitochondrial complex I Experimental procedures Cell lines and cell culture The isolation and preliminary biochemical and genetic characterization ... used in a Western analysis with antisera against the HA epitopes It is noteworthy that the epitope tags not interfere significantly with the incorporation of the tagged ESSS subunit into a functional...
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Tài liệu Báo cáo khoa học: Phylogenetic relationships in class I of the superfamily of bacterial, fungal, and plant peroxidases pptx
... peroxidase Catalase–peroxidase Catalase–peroxidase Catalase–peroxidase Catalase–peroxidase Catalase–peroxidase Catalase–peroxidase Catalase–peroxidase Hypothetical protein Ascorbate peroxidase ... Q8EIV5 O46921 Ascorbate peroxidase Ascorbate peroxidase (thylakoid) Catalase–peroxidase Catalase–peroxidase Catalase–peroxidase Catalase–peroxidase Catalase I Catalase–peroxidase Catalase–peroxidase ... (Rhodophyta) Catalase–peroxidase Catalase–peroxidase Ascorbate peroxidase Ascorbate peroxidase Catalase–peroxidase Catalase–peroxidase Catalase–peroxidase Ascorbate peroxidase (thylakoid) Ascorbate...
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Tài liệu Báo cáo khoa học: Effect of deletion of the DNase I hypersensitive sites on the transcription of chicken Ig-b gene and on the maintenance of active chromatin state in the Ig-b locus docx
... region I, in vivo activity of region IV did not correlate with the activity observed in transient transfection assays These observations demonstrate that regions II, III, and IV are involved in the ... by a black rectangle Size and position of the HindIII fragment hybridizable with the probe is shown below the map Exons and introns are indicated as in Fig (B) Genomic Southern hybridization HindIII ... kHindIII Size of the bands observed in each autoradiogram is indicated at the right side The 6.6 kb band detected in lanes b, c, and d in autoradiogram (B), and lanes c and d in (C) is derived...
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Tài liệu Báo cáo khoa học: Characterization of ICAM-4 binding to the I domains of the CD11a/CD18 and CD11b/CD18 leukocyte integrins pptx
... [14,38] A mouse IgG1 negative control was purchased from Silenius (Hawthorn, Australia) and polyclonal goat anti-GST antibodies from Pharmacia Biotech Inc The goat antihuman IgG specific antibody was ... inhibition of binding of ICAM-4 positive red cells by anti-ICAM-4 antibodies, indicate a major role for ICAM-4 in binding of red cells to the I domains The efficient inhibition of the 1720 E Ihanus ... domain GST, the majority of the CD1 1a I domain GST was found in the insoluble fraction of the E coli lyzate Modification of the solubilization conditions and increasing the volumes of the bacterial...
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Tài liệu Báo cáo Y học: Role of electrostatics in the interaction between plastocyanin and photosystem I of the cyanobacterium Phormidium laminosum ppt
... was based on the transition state theory of Eyring [37] The interpretation of the activation parameters in Table depends on whether the reaction is diffusionlimited or activation-limited In the ... interaction of plastocyanin with photosystem I in Anabaena [15], and is highly conserved in cyanobacterial plastocyanins Mutagenesis, expression, purification and characterization of the plastocyanins ... oxidase, another potential reaction partner of plastocyanin in cyanobacteria It remains to be clarified if the acidic residues serve to discourage futile reactions (e.g with photosystem II) and/or...
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Báo cáo khoa học: Comparing the substrate specificities of cytochrome c biogenesis Systems I and II docx
... Supporting Information) Although it is possible that the CXXAH variant of P denitrificans cytochrome c550 is unstable and cannot be made, the two single-cysteine-containing (and AXXAH) variants ... appropriate antibiotics Growth on solid medium used Luria–Bertani medium supplemented with 1.5% bacteriological agar For the preparation of periplasmic fractions, single colonies containing appropriate ... might be anticipated [21,31,40], that the single-cysteine variant in which the heme-binding cysteine is directly adjacent to the heme iron-ligating histidine (i. e XXXCH) is the more likely to...
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Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
... algal loop contains a similar distribution of positive charges, although fewer than Arabidopsis (4 instead of 6) Aside from the charge distributions, the loops exhibit enough variation to leave ... or 5¢-TGGAGCCACCCGCAGTTCGAAAAAGAA GCTGGAGATGATCGTGCT-3¢ (Strep-tag underlined), then a secondary amplification with primers 5¢-CACCAC CACCACCACCACGAAGCTGGAGATGATCGTGCT-3¢ (His-tag underlined) or ... (B) Insertion of Chlamydomonas reinhardtii PSI-G into thylakoids (Lanes as in panel A) Alignment of the loop region of Arabidopsis and Chlamydomonas PSI-G Positively charged amino acids are shown...
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Báo cáo Y học: Characteristics of binding of insulin-like growth factor (IGF)-I and IGF-II analogues to the type 1 IGF receptor determined by BIAcore analysis pptx
... disrupt IGFBP binding and were therefore expected to maintain rhIGF1R-binding af®nities similar to IGF -I Long Gly3-IGF -I and Long Arg3-IGF -I also had similar rhIGF1R-binding af®nities to IGF -I ... obtained from BIAcore analysis of binding of IGF -I, IGF-II and mutant IGF analogue to rhIGF1R Data were analysed using BIAevaluation software 3.0 and ®tted to a Langmuir : binding model as outlined in ... Kd of IGF -I/ Kd of IGF analogue Dashes indicate data inappropriate for assessing association and dissociation rates No detectable binding (ND) was seen with Ala31-IGF -I (800 nM), Leu60-IGF -I (800...
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Báo cáo Y học: Properties of group I allergens from grass pollen and their relation to cathepsin B, a member of the C1 family of cysteine proteinases pdf
... expressed in the yeast Pichia pastoris, catalyzed the degradation of a synthetic substrate containing a papain-cleavage site, as well as other proteins Moreover, a protein with strong proteolytic activity ... at a papaincleavage site after incubation under acidic and reducing conditions, which are known to activate C1 proteinases At that time, limited sequence similarity to motifs surrounding the active-site ... comparable location enhances the significance of the sequence similarities Other functional amino acids in cathepsins are also conserved in most or all expansins First, the distribution of cysteine...
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