... settings of the Lightcycler Software Version 3.5.3 and conditions in the linear range of the PCR-reaction were ensured Cell type expression of spherulin 3b and polymalatase Quantitative analysis of ... identified to date Transcript of NKA48 showed the highest abundance and was further analysed Nucleotide and deduced amino acid sequences of NKA48 were compared with the GenBank database The results indicated ... loss of induction at the transcriptional level, of loss of activation of the synthetase protein itself, or of derepression of enzyme(s) catalysing PMLA degradation An interesting interplay of NKA48...
Ngày tải lên: 07/03/2014, 12:20
... measure of the costs of climate change is the rising costs of extreme weather events, though these are also a function of and real estate and insurance values Overall, the costs of weather-related ... Plant-by-plant estimates of the damages ranged from 1.9 ¢/kWh to 12 ¢/kWh Plant-to-plant variation was largely due to controls on the plant, characteristics of the coal, and the population downwind of the ... occur when the activity of one agent affects the well-being of another agent outside of any type of market mechanism—these are often not taken into account in decision making and when they are...
Ngày tải lên: 23/03/2014, 00:20
Báo cáo y học: "A role for the histone deacetylase HDAC4 in the life-cycle of HIV-1-based vectors" ppsx
... http://www.virologyj.com/content/7/1/237 Page of 10 Figure Effect of an integrase inhibitor on the formation of HDAC4 foci in infected cells HeLa cells were infected with the HIV-1-based vector in the presence and absence of the integrase ... for these steps of the retroviral life- cycle Nevertheless, it is possible that HDAC4 affects the life- cycle in different ways One possibility is that HDAC4 has a cell-type-specific function and ... stimulates the association of HDAC4 with vector DNA and wondered if integration affects the formation of HDAC4 foci Thus, we have infected HeLa cells in the presence and absence of an integrase...
Ngày tải lên: 12/08/2014, 01:21
Environmental Impact of Products (EIPRO) Analysis of the life cycle environmental impacts related to the final consumption of the EU-25 pot
... at the core of the IPTS’s development strategy Only through its networks can an institute the size of the IPTS hope to provide high-quality advice at the European level over the whole range of ... the Netherlands Gjalt Huppes, Jeroen Guinée, Reinout Heijungs, Arjan de Koning, Lauran van Oers, and Sangwon Suh CML, Leiden University, the Netherlands Theo Geerken, Mirja Van Holderbeke, and ... products on the basis of the overall volume of the product used Take account of the impact per euro Focus primarily on the life cycle impacts of products (both goods and services) in terms of final...
Ngày tải lên: 23/03/2014, 08:21
Báo cáo hóa học: " Development and application of a biomarker assay for determining the pharmacodynamic activity of an antagonist candidate biotherapeutic antibody to IL21R in whole blood" pptx
... performed all the studies on human samples reported here, analyzed the data and co-wrote the manuscript SJ and AAW performed all the pilot studies in humans and monkeys that identified the candidate ... data analysis and review and participated in the drafting of the manuscript DY co-led the Ab-01 team and was responsible for studies related to the characterization of the biological properties of ... observed in both humans and monkeys The presence of IL21R on the top gene list for monkeys and not for humans reflects a higher relative ranking in monkeys due to the lack of IFNγ and CD19 data in...
Ngày tải lên: 18/06/2014, 16:20
Báo cáo y học: " Repetition and severity of suicide attempts across the life cycle: a comparison by age group between suicide victims and controls with severe depression" ppsx
... acts The aim of the present study was to compare the incidences of suicide attempt during the entire lifespan by age group in suicide victims and controls, with gender taken into consideration The ... 2006 Out of these 103 had taken their life up to 1984, another 11 up to 1998, and more up to 2006 Deceased persons were grouped according to the primary cause of death as classified by the Swedish ... evaluation The entire course of depression up to the death of the suicide victims and a corresponding date for the matched controls was studied There were a total of 1,505 observation years in the suicide...
Ngày tải lên: 11/08/2014, 17:20
Báo cáo y học: " The Cell Cycle Ontology: an application ontology for the..." pdf
... (classes) of instances (individuals) Classes can be subclasses of other classes, if and only if all the instances of the subclass are also instances of the superclass, although the superclass has other ... along the property 'part _of' to an instance of the class 'Cell' (other quantifiers can be used in these restrictions such as 'only', 'min', 'max' and 'value', and Boolean operators such as 'and', ... networks With a right click of the mouse on any of the nodes shown by the applet, the user can ask for the local neighborhood of a term in the network, for the path to the root and for extra information...
Ngày tải lên: 14/08/2014, 21:20
Tài liệu Characterization of the Polymorphic Behavior of an Organic Compound Using a Dynamic Thermal and X-ray Powder Diffraction Technique pptx
... characterize the polymorphic behavior of an organic compound API over a temperature range of hundreds of degrees This allows the simultaneous measurements of thermochemical and thermophysical ... (see Figures and 9) The lack of any additional peaks in the XRPD patterns would imply the presence of an Figure DSC of atypical anhydrous Form I (sample 11) amorphous material and/or an impurity ... through the center of the sample and reference sensors of the DSC cell Thermal isolation was maintained by using beryllium metal foil to seal the X-ray optical path identification and understanding of...
Ngày tải lên: 14/02/2014, 03:20
Tài liệu Báo cáo khoa học: Understanding the binding properties of an unusual metal-binding protein ) a study of bacterial frataxin pdf
... from the other Disappearance of some resonances without the obvious appearance of new signals suggests the presence of an intermediate rateexchanging equilibrium between the resonances of the ... disappearance of specific resonances without the concomitant appearance of other signals in other parts of the spectrum This result could be a consequence of the paramagnetic properties of Fe(II), ... rather than with specific binding Aggregation seems to initiate around the first helix Titration of CyaY with lanthanides ) the effect of europium and ytterbium Lanthanides were used to enhance the...
Ngày tải lên: 18/02/2014, 16:20
Tài liệu Báo cáo khoa học: Dimer asymmetry and the catalytic cycle of alkaline phosphatase from Escherichia coli doc
... enhances a conformational change inducing the release of the product and Mg2+, thereby leaving an Mg2+ ion and a molecule of the substrate bound to the subunit capable of catalyzing hydrolysis Therefore, ... conformational change could be induced or enhanced by any ligand with a different binding affinity for subunits and If the ligand concentration is higher than that of the substrate, the conformational change ... by an interaction with an acceptor protein or a transmembrane channel In case of APase it would allow simultaneous diffusion of Mg2+ and Pi into the cell It has been shown that the PiT transport...
Ngày tải lên: 21/02/2014, 00:20
Tài liệu Báo cáo khoa học: Coordination chemistry of iron(III)±porphyrin±antibody complexes In¯uence on the peroxidase activity of the axial coordination of an imidazole on the iron atom ppt
... the antibody and particularly to appreciate the size of the cavity left around the iron atom and which kind of ligands it can accommodate; second, to measure the in¯uence of an axial ligand of ... (Table 1) The binding of the ®rst CN± ligand to the iron was thus more easy in the hydrophobic binding pocket of the antibody than the binding of the second one, most probably because of the steric ... bound to the iron, the steric hindrance due to the substituent could be balanced by a distortion of the porphyrin ring and a shift of the iron atom outside the plane of the porphyrin Binding of imidazole...
Ngày tải lên: 21/02/2014, 03:20
Tài liệu Báo cáo Y học: Evolution of the enzymes of the citric acid cycle and the glyoxylate cycle of higher plants pdf
... isoenzymes of animals and plants, the plastid NADP+-speci®c isoenzymes of plants, and several interleaving eubacterial homologues In contrast with fungi, the cytosolic MDHs of animals and plants fall ... analysis of the evolution of these enzymes than we have performed here However, our aim was not to perform an exhaustive analysis but to obtain an overview of the patterns of similarity for the ... from the cyanobacterial ancestor of plastids The E1a and E1b subunits of chloroplast PDH are even still encoded in the chloroplast genome of the red alga Porphyra [41], the genes having been transferred...
Ngày tải lên: 22/02/2014, 04:20
Báo cáo khoa học: The catalytic mechanism of dye-decolorizing peroxidase DyP may require the swinging movement of an aspartic acid residue potx
... H2O2 and CN, the position of the carbon atom of CN bound to the heme iron mimics the position of the proximal oxygen of H2O2 bound to the heme iron during compound I formation Therefore, the carbon ... achieved with the hang- Fig The swinging mechanism of Asp171 (A) Interpretation of distances between Asp171 and a virtual proximal oxygen of H2O2 in the native and D171N-CN The native and D171N-CN ... the heme planes Note that Asn171 of D171N-CN is shown as Asp171 To avoid misunderstanding, only the heme of the native is shown in white The position of the CN carbon mimics the position of the...
Ngày tải lên: 06/03/2014, 00:20
the accidental death of an anarchist
... political amalgamation of lies aimed at hiding the truth behind the anarchist's death There were many themes presented in ADOA including that of the abuse of power by the authoritarian This occurs when ... By breaking down the fourth wall, the audience becomes co-conspirators, and part of the political conspiracy Through the Maniac's exaggerated and comic view of the court system, the responder also ... of the truth i.e the Italian police and the corrupt nature of society This is shown when the Maniac ridicules the police by burning and throwing out documents, mocking the way the police destroy...
Ngày tải lên: 21/03/2014, 22:52
Báo cáo khoa học: The role of residues R97 and Y331 in modulating the pH optimum of an insect b-glycosidase of family 1 pdf
... deglycosylation) of the hydrolysis of MUbglc by the mutant enzymes is rate-limiting, no hypothesis on the effect of R97 and Y331 on the rate constant of each step can be advanced ể FEBS 2003 Mechanism of ... As the pKa values of the catalytic residues determine the pH optima, the ionization constants (pKa) of the catalytic glutamates (E187 and E399) in the ES complex and in the free enzyme (E) of ... b-glucosidase affected the rate constant of the glycosylation step and also changed the enzyme pH optimum [14] Despite that, the effect of the tyrosine on the pKa values of the b-glycosidase catalytic...
Ngày tải lên: 23/03/2014, 15:21
hack the stack - using snort & ethereal to master the 8 layers of an insecure network
... to another Port 20 is used for the data stream, and transfers the data between the client and the server Port 21 is the control stream, and is used to pass commands between the client and the ... could use the whistle to signal a new call and then dial anywhere in the world for free The Application Layer Layer is known as the application layer Recognized as the of cial top layer of the OSI ... attacks, the bulk of the work of a social engineering attack is doing the reconnaissance and laying the groundwork .The attack itself usually takes on one of the following angles: ■ Diffusion of Responsibility...
Ngày tải lên: 25/03/2014, 11:19
Báo cáo khoa học: The influence of temperature and osmolyte on the catalytic cycle of cytochrome c oxidase ppt
... become anaerobic The time course of the absorbance changes at 276 K (d) and 250 K (s) are shown in (A) and (B) based on the data of the absorption spectra of Figs and In both panels, the data ... fit to the data The R2 for the fit was better than 0.998; the fit is shown as the solid line through each set of data points Fig The time course of the absorbance changes at (A) 276 K (d) and (B) ... 443 nm and that which disappears at 416 nm The spectra of Figs and are compared in Fig where the emphasis is on the changes occurring at 443 nm and 605 nm The progression to and out of the steady...
Ngày tải lên: 31/03/2014, 07:20
Báo cáo Y học: The DNA-polymerase inhibiting activity of poly(b-L-malic acid) in nuclear extract during the cell cycle of Physarum polycephalum pot
... Materials and methods, the degree of inhibition depends on both the concentration of free PMLA and the inhibition constant This parameter reflected the affinity of the polymerase and both the affinity and ... inhibitory activity is then (vo ) vi)/vi ¼ [PMLA]/Ki Therefore, the higher the concentration of free PMLA, and the lower the value of the inhibition constant (Ki), the higher the relative inhibitory ... the catalytic competence of DNA polymerases throughout the giant cell We are currently investigating the transfer of PMLA between the nuclei, and the competitive exchange of DNA polymerases and...
Ngày tải lên: 31/03/2014, 21:21
numerical analysis of the spectral response of an nsom measurement
... gives the magnitude of the energy flow and its direction Figure shows the sum of the energy density stored in the electric and magnetic field and the magnitude of the Poynting vector at the center of ... respectively The variance of the permittivity shown in Fig is reflected in both the near and far-fields and the peaks from the PEC model are dra- Numerical analysis of the spectral response of an NSOM ... is that the E field is orthogonal to the H field and scaled by η, the impedance of the medium This allows easy calculation of the radiated power The far-field response has both an angular and spectral...
Ngày tải lên: 06/05/2014, 08:58
university of texas press thelma & louise live the cultural afterlife of an american film nov 2007
... take advantage of the sweep of the American West, situating the personal development of the characters into the visual iconography of the American cinema and history In Thelma & Louise: The Last ... availability, the absence of civilization, and so hides the histories of the West in favor of the familiar fantasies of the West as an unoccupied place of self-creation for Anglo tourists (like their ... image of an empty two-lane blacktop road, emerging from the bottom center of the poster and leading toward an image of Monument Valley, a horizon of mesas and rocks across the lowest quarter of the...
Ngày tải lên: 11/06/2014, 13:58