somatic stem cells definition biology

Ngày tải lên: 28/06/2014, 05:20

Ngày tải lên: 09/08/2014, 10:20

... Flk-1+CD31-CD34- mesenchymal stem cells: feasibility and safety from monkey to human. Stem Cells Dev,2006,15(3):349-357 41. Devine SM, Bartholomew AM, Mahmud N, et al. Mesenchymal stem cells are capable ... of stem cells, it is very important to observe the survival and cura- tive effects of transplanted stem cells. Traditional immunohistochemical method can easily identify the transplanted cells ... circulation in the necrotic femoral head and sur- rounding tissues [4]. Mesenchymal stem cells (MSCs) are multipotent stem cells that can differentiate into a variety of cell types. In the field of...

Ngày tải lên: 25/10/2012, 11:18

... break-down and power failure. Key words: human embryonic, stem cells, low temperature 1. Introduction In vitro culture of human embryonic stem cells (hESC) often involves temporary exposure to reduced ... efficient and safe xeno-free cryopreservation method for the storage of human embryonic stem cells. Stem Cells. 2004;22(5):779-89. 10. Speicher MR, Ballard SG, Ward DC. Karyotyping human chromosomes ... some cells detached after exposure to low temperature. Virtually all of the detached cells were determined to be non-viable with tryphan blue staining (data not shown). All of the detached cells...

Ngày tải lên: 31/10/2012, 16:57

... mesenchymal stem cells. Science 284, 143–147. 2 Porada CD, Zanjani ED & Almeida-Porad G (2006) Adult mesenchymal stem cells: a pluripotent population with multiple applications. Curr Stem Cell ... (2007) Effect of hypoxia on gene expression of bone marrow- derived mesenchymal stem cells and mononuclear cells. Stem Cells 25, 1166–1177. 7 Li L, Zhang S, Zhang Y, Yu B, Xu Y & Guan Z (2009) ... mesenchymal stem cells (MSCs) are easily obtainable and expandable, multipotent progeni- tor cells [1] that have emerged as attractive candidates for cellular therapies for heart and other organ-system disorders...

Ngày tải lên: 18/02/2014, 04:20

... triggering. There is another example of stemness decisions by Nodal versus BMP signaling emerging from human embryonic stem cell research. Testing human ES -cells BMP signaling in stem cells T. U. Wagner 2972 ... embryonic stem cells. Stem Cells 22, 770–778. 22 Ying QL, Nichols J, Chambers I & Smith A (2003) BMP induction of Id proteins suppresses differentiation and sustains embryonic stem cell self-renewal ... signals in stem cells Bone morphogenetic protein (BMP) signals have tre- mendous effects on all kinds of cells. Most striking and defining, however, are the reactions that stem and progenitor cells...

Ngày tải lên: 19/02/2014, 00:20

... murine ES cells and in human hematopoietic progenitor cells. Discussion The commitment of pluripotent stem cells to succes- sively less plastic progenitors and, finally, differentiated cells exhibiting ... murine embryonic stem cells and in human CD 133+ bone marrow cells. (A) Transcription of LCR regions and the b maj -globin gene during differentiation of erythroid cells from murine ES cells. RNA was ... differentiation of murine embryonic stem cells. PCR analysis of DNase I treated and reverse-transcribed total RNA extracted from differentiating embryonic stem cells at the indicated time points....

Ngày tải lên: 07/03/2014, 12:20

... assay in stem cell biology. Nature methods. 2006;3(10):801-6. [54] Jessberger S, Clemenson GD, Jr., Gage FH. Spontaneous fusion and nonclonal growth of adult neural stem cells. Stem Cells. 2007;25(4):871-4. [55] ... some Nestin-expressing Type-1 cells may exit the stem cell pool, clones were found that contained only differentiated cells. Taken together these data indicate heterogeneity within the stem cells pools and ... horizontal cells could reactivate in the aged brain or whether a Neural Stem Cell Heterogeneity http://dx.doi.org/10.5772/55676 37 Contents Preface VII Section 1 Neural Stem Cells as Progenitor Cells...

Ngày tải lên: 08/03/2014, 19:20

... ã Can human embryonic stem cells differentiated into brain cells and inserted into mouse brain make the mouse think like a human? ã Outcome If human stem cells were inserted into developing ... points: – 8  – # – !#4  – =#%+& Stem Cells: Stem Cells: Ethical and Regulatory Ethical and Regulatory Issues Issues Sidney H. Golub, Ph.D. Chair, UC Irvine Human Stem Cell Research Oversight ... alternative to ESCs. ã Fetal cells allowed for transplants (1993). Sale of fetal/embryonic materials prohibited (2006). Stem Cells Adapt for Many Parts of the Body Stem Cells Pancreas Brain Heart Bone...

Ngày tải lên: 13/03/2014, 16:29

... cells derived from cultured mouse ESCs are fat cells, various brain and nervous system cells, insulin-producing cells of the pancreas, bone cells, hematopoietic cells, yolk sac, endothelial cells, ... adult stem cells is the relationship of the cellular environment to the concept of plasticity in adult stem cells. Markus Grompe showed that HSCs and pancreatic stem cells can give rise to liver cells ... BASIC DEFINITIONS Stem cells are unspecialized cells that can self-renew indefinitely and that can also differentiate into more mature cells with specialized func- tions. In humans, stem cells...

Ngày tải lên: 15/03/2014, 09:20

... pluripotent stem cells ISCN International system for human cytogenetic nomenclature IVF In vitro fertilization KO Knockout 1 M. Amit and J. Itskovitz-Eldor, Atlas of Human Pluripotent Stem Cells: ... Culturing, Stem Cell Biology and Regenerative Medicine, DOI 10.1007/978-1-61779-548-0_1, â Springer Science+Business Media, LLC 2012 Abstract Human embryonic stem cells (hESCs) are pluripotent cells ... Lanza R (2005) Human embryonic stem cells derived without feeder cells. Lancet 365:1636–1641 Series Editor Kursad Turksen, Ph.D. kturksen@ohri.ca Stem Cell Biology and Regenerative Medicine For...

Ngày tải lên: 22/03/2014, 09:20

... Leydig cells, prospermatogonia enter rounds of mitosis, generating spermatogonial stem cells. From within this reservoir of self-generating stem cells emerge, at intervals, groups of cells with ... The cells within these colonies were of three types; Oct-4-only positive cells, Oct-4- and Vasa-positive cells, and Vasa-only positive cells. This suggested that the in vitro– derived germ cells ... field, however, was the production of germ cells and putative oocytes from somatic stem cells (5,6). Porcine fetal skin stem cells cultured with 5% porcine follicular fluid resulted in aggregates...

Ngày tải lên: 22/03/2014, 20:21

... It ICM TE PGC TS cells ES cells EG cells 4977 Placental cells Embr y onic cells Fig. 1. Epigenetic distances between ES cells and other stem cells derived from developing embryos. ES cells derived from the ... between ES and other stem cells Comparing ES cells with other stem cells established from developing embryos revealed the uniqueness of the epigenetic profile in ES cells. In contrast to ES cells, which ... ES cells (77) is greater than that of EG cells to ES cells (49), confirming the similarity of EG cells to ES cells. Epigenetic study of embryonic stem cells N. Hattori and K. Shiota 1626 FEBS...

Ngày tải lên: 23/03/2014, 07:20

... adipose tissue-derived mesenchymal stem cells (AT-MSCs) differentiate into hepatocyte-like cells upon treatment with hepatocyte growth factor (HGF), oncostatin M and dimethyl sulfoxide [13]. These cells expressed ... differen- tiation from stem cells. For microarray analysis, we confirmed the hepatic differentiation of AT-MSC into hepatocyte-like cells using the original protocol (Fig. 1A). The differentiated cells (AT-MSC-Hepa) ... mesenchymal stem cells into hepatocyte- like cells. Biochem Biophys Res Commun 330, 1153–1161. 13 Seo MJ, Suh SY, Bae YC & Jung JS (2005) Differentia- tion of human adipose stromal cells into...

Ngày tải lên: 30/03/2014, 04:20

... glial fibrillary acidic protein-positive cells from the adult central nervous system results in the loss of forebrain neural stem cells but not retinal stem cells. Eur J Neurosci, Vol. 18, No. ... maintenance of SVZ stem cells (Lehtinen et al. 2010). Stem and progenitor cells have also been identified in the peripheral nervous system, i.e., in the carotid body, the enteric nervous system and the ... between cells with astrocytic phenotype (type-2a cells, the early stage of type-2 cells) and cells with early features of the neuronal lineage (type-2b cells, the later stage of type-2 cells) ....

Ngày tải lên: 30/03/2014, 23:20

... 26 TCNCYH phụ bản 32 (6) - 2004 13 Tế bào gốc và ứng dụng trong y sinh học (Stem cells and the application in biomedicine) Nguyễn Thị Thu Hà* Bệnh viện Trung ơng Quân đội 108 ... bào thai ngời (Human Embryonic Stem cell) Năm 1998 nhóm nghiên cứu của James Thomson đà phân lập và duy trì đợc một loại tế bào gốc bào thai (ES: embryonic stem cell) từ khối tế bào bên trong ... ES và EG đợc gọi chung là tế bào gốc bào thai. iii. Tế bào gốc của cá thể trởng thành (adult Stem Cell): Tế bào gốc của cá thể trởng thành cũng giống nh tất cả những tế bào gốc khác mang...

Ngày tải lên: 02/04/2014, 22:20