pharmacogenetics of nuclear receptors

... List of nuclear receptors screened for enhancers of reprogramming… … 62 Table Pluripotent assays of Nr5a2-reprogrammed cells……………………… …74 x List of Figures Figure The developmental potential of ... SCREEN OF NUCLEAR RECEPTORS FOR THE ENHANCED AND ALTERNATIVE GENERATION OF INDUCED PLURIPOTENT STEM CELLS DOMINIC HENG JIAN CHIEN (B.Sc (Hons), NTU) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF ... Figure Reprogramming of somatic cells with transcription factors…………… 14 Figure Stages of reprogramming to mouse iPSCs………………………… …….20 Figure Mechanism of action of nuclear receptors ………………………………40...

Ngày tải lên: 09/09/2015, 18:56

... development of cancer therapies 2 Nuclear Receptors 2.1 Classification of nuclear receptors 2.2 Nuclear receptors as modular proteins 2.2.1 Nuclear receptor co-factors 2.2.2 Specificity of target ... investigate the role of each of the plenitude of genes and proteins important in the progression of cancer NUCLEAR RECEPTORS The nuclear receptors are a superfamily of structurally conserved transcription ... 2005) 2.2.2 Specificity of target genes The promoters of the target genes of nuclear receptors possess a hormone response element (HRE) and the DNA binding domain of nuclear receptors recognize it...

Ngày tải lên: 14/09/2015, 14:04

... promoter by the nuclear receptors heterodimer is dependent upon ligand binding leading to the binding of the nuclear receptors to the response elements (REs) in the 5’ flanking region of CYP3A4 Transactivation ... Comparison of SNP frequencies of HNF4α exon 1C variant………… 59 Table 13 Comparison of SNP frequencies of HNF4α exon variant……………59 Table 14 Genotypic distribution and allele frequencies of PXR, CAR ... Figure The structure of HNF4α gene and its spliced isoforms……………… 22 Figure An illustration of the effects of docetaxel in tumour cell…………… 27 Figure Proposed metabolic pathways of docetaxel by CYP3A...

Ngày tải lên: 09/10/2015, 11:06

... 3.2 Basal Activity of promoter constructs .36 3.3 Nuclear Receptor Expression Profiles of HepG2, Huh7 and MCF7 cell lines 37 3.4 Cytotoxicity of activators of nuclear receptors to cell ... Unpublished 1.3 Nuclear receptors Nuclear receptors are a class of intracellular proteins that regulate the sensitizing of hormones and certain molecule The cloning of these receptors took place ... relevant nuclear receptors of interest The MCF7 cells were used as these cells express the estrogen receptors and allow for the study of the effects of estrogen 2.3 Prediction of Putative Nuclear...

Ngày tải lên: 22/10/2015, 22:37

... comparison of ecdysteroid receptors A B Fig CLUSTALW amino acid alignment of N-terminal (A ⁄ B) domains of LdEcR and DmEcR (A) Alignment of EcRA from the two species (B) Alignment of DmEcRB1, ... alignment of linker region and ligand-binding domain (LBD) of ecdysone receptors Fig S2 Amino acid alignment of linker region and ligand-binding domain (LBD) of ultraspiracle ⁄ RXR receptors ... by isoforms of the ecdysone receptor and inhibitory effects of E75A and MHR3 Dev Biol 272, 510–21 Azotei A & Spindler-Barth M (2009) DNA affects ligand binding of the ecdysone receptor of Drosophila...

Ngày tải lên: 18/02/2014, 08:20

... characterization of nuclear receptors from the red flour beetle, Tribolium castaneum Insect Biochem Mol Biol 38, 430–439 Bonneton F, Chaumot A & Laudet V (2008) Annotation of Tribolium nuclear receptors ... established a lack of fluctuation in the protein levels of AaEcR and AaUSP isoforms during vitellogenesis [13], suggest a lack of significant transcriptional and translational regulation of the two components ... has three isoforms, each with a distinctive N-terminal A ⁄ B domain [36] AaE75B cannot bind DNA due to the lack of one of its zinc fingers All three isoforms present a similar pattern of expression...

Ngày tải lên: 18/02/2014, 13:20

... alters the expression of the two nuclear genome coded isoforms of CytOX V [10,32] The regulation of genes CytOX5a and CytOX5b, coding for the two isofoms Va and Vb, parallels that of genes CYC1 and ... to suppression of respiration during hypoxia In the absence of differential regulation of a specific nuclear gene coding for the subunits of CytOX, changes in the microenvironment of the cell may ... modulates the expression of mRNAs for CytOX subunits and also the catalytic activity of the enzyme complex Our results show that the levels of some of the select mitochondrial and nuclear genome encoded...

Ngày tải lên: 20/02/2014, 23:20

... inhibition of nuclear pre-mRNA splicing by any antibiotic has not been described so far Here, we report on the inhibitory actions of erythromycin, Cl-tetracycline and streptomycin on nuclear splicing of ... graph The eciency of lariat formation (%) is plotted as a function of increasing concentrations of antibiotic (lM) (D) Inhibition of b-globin splicing by increasing concentrations of erythromycin ... vitro as inhibitors of nuclear b-globin pre-mRNA splicing (Fig 2) They are members of three different classes of antibiotic: macrolides, tetracyclines and aminoglycosides Of the antibiotics tested,...

Ngày tải lên: 21/02/2014, 15:20

... this mode of decay, the atomic number of the daughter nuclide is one more than that of the parent with no change in mass number The mass of the neutron is more than the sum of masses of the proton, ... of 99Mo 100 50 Growth of 99mTc T1 ð1 À e T1 À T2 Radioactivity A2 ¼ A1 Growth of 113mIn The solution of this equation in terms of activity can be given as follows: Radioactivity (1.9) Decay of ... transfer of energy during the interaction of the photon with an atom of the medium where E0 is the energy of the incident photon and E1 is that of the scattered photon, me is the mass of the electron,...

Ngày tải lên: 06/03/2014, 09:21

... early stages of assembly that involve formation of the catalytic core The remaining nDNA-encoded subunits are thought to be added during the last steps of assembly; however, this part of the assembly ... represents an important step in assembly of the holoenzyme Results In vitro import and assembly of nDNA-encoded complex IV subunits The assembly of a number of nDNA-encoded complex IV subunits was ... progression of intermediates along the assembly pathway due to the use of isolated mitochondria Our results are consistent with those of a previous study that analyzed the assembly of yeast complex...

Ngày tải lên: 07/03/2014, 00:20

... be mediated by P2X receptors Activation of P2X5 receptors stimulated the differentiation of skin cancer cells with subsequent inhibition of proliferation [16], and induction of cell death was ... Inhibition of proliferation of LXF-289 lung tumor cells Effects of P2 receptor agonists The influence of the different P2 receptor agonists is shown as percentage inhibition of the proliferation of LXF-289 ... factor of proliferation and apoptosis Attenuation of LXF289 cell proliferation by extracellular ATP is mediated by the induction of nuclear translocation of p50 NF-jB1 and possibly new formation of...

Ngày tải lên: 07/03/2014, 12:20

... under the control of the GAL10 promoter The induction of the yeast, by galactose containing media, results in overexpression of GAL4, in turn inducing an increase of the expression of the OR gene ... 2004 FEBS Expression of olfactory receptors in yeast for screening cells may provide a means for detailed investigation of receptor pharmacology in vivo through the use of sensitive reporter ... mutation that prevents cell cycle arrest upon activation of the pheromone525 Expression of olfactory receptors in yeast for screening Fig mRNA of the rat I7 OR or human OR17-40 receptor in transformed...

Ngày tải lên: 07/03/2014, 16:20

... S1157 is the area of 1157 keV one Determining the isomeric ratio of nuclear reaction 55 3.3 Isomeric ratio The detection-efficiency of gamma spectrometry with energy of 271 keV and of 1157 keV has ... intensity of gamma ray with energy Eγ = 271keV and with energy Eγ = 1157keV is 86.7 : 1.31 So that, the count of peak with 271keV energy of metastable state is equal the area of spectral peak of 271keV ... − e−λm t3 )Cm (2.3) Determining the isomeric ratio of nuclear reaction 53 Similarly, the area of spectral peaks caused by the disintegration of nuclei Sg =Ng =fg g N0 φ0 Pm,g γ +fg g N0 φ0 γ...

Ngày tải lên: 14/03/2014, 13:20

... S1157 is the area of 1157 keV one Determining the isomeric ratio of nuclear reaction 55 3.3 Isomeric ratio The detection-efficiency of gamma spectrometry with energy of 271 keV and of 1157 keV has ... intensity of gamma ray with energy Eγ = 271keV and with energy Eγ = 1157keV is 86.7 : 1.31 So that, the count of peak with 271keV energy of metastable state is equal the area of spectral peak of 271keV ... − e−λm t3 )Cm (2.3) Determining the isomeric ratio of nuclear reaction 53 Similarly, the area of spectral peaks caused by the disintegration of nuclei Sg =Ng =fg g N0 φ0 Pm,g γ +fg g N0 φ0 γ...

Ngày tải lên: 14/03/2014, 13:20

... T-ag UDE of D melanogaster TDG of D melanogaster v-Jun of Homo sapiens dUTPase of Homo sapiens H2B of Saccharomyces cerevisiae c-Myc of Homo sapiens dUTPase of D melanogaster c-Myc of Xenopus ... with its putative nuclear function in insect cells Subcellular distribution of C-terminal truncated forms of UDE To test whether the nuclear import of UDE requires any or both of the predicted ... the case of the short isoform (NTT-DUT), absence of the first 14 residues of the N-terminus, including the PAAKK(10–14) segment, dramatically alters the translocation pattern of dUTPase Nuclear...

Ngày tải lên: 15/03/2014, 11:20

... Court of Justice on the legality of the threat of use of nuclear weapons 18 Legality of the Use by a State of Nuclear Weapons in Armed Conflict, Advisory Opinion, Jul1996, ICJReports 1996, p 66 (Nuclear ... the question of the (il)legality of the employment of nuclear weapons from the point of view of international environment protection law By assessing the legality of the use of nuclear weapons ... illegality of the use of nuclear weapons In operative para 1, the Assembly declared: ‘(a) The use of nuclear and thermo -nuclear weapons is contrary to the spirit, letter and aims of the United...

Ngày tải lên: 16/03/2014, 13:20

... direct evidence of the involvement of PPARa in the activation step of CAR Another possibility for the indirect but essential involvement of PPARa in the induction is that some of them are regulated ... FJ (1995) Targeted disruption of the a isoform of the peroxisome proliferator-activated receptor gene in mice results in abolishment of the pleiotropic effects of peroxisome proliferators Mol ... (2000) Use of the nuclear receptor PXR to predict drug interactions Toxicology 153, 1–10 33 Motojima K (2000) Differential effects of PPARa activators on induction of ectopic expression of tissue-specific...

Ngày tải lên: 16/03/2014, 14:20

... from the presence of Dr L Biro, Minister Secretary of State with Nuclear Safety, who presented a documented image of the nuclear energy in Romania and emphasized the importance of a durable development ... 3,4He) emitted in neutron of interest [5] for evaluation of nuclear data for D on 6'7Li The basic input for the calculations of the double-folded OP are the nuclear densities of the colliding nuclei ... Sn A spectrum of the 234 U halflives versus the mass and atomic numbers of the fragments illustrates the idea of a unified treatment of different decay modes over a wide range of mass asymmetry...

Ngày tải lên: 17/03/2014, 20:21

... changed by any of the four types of CRLPs Effect of the fatty acid composition of CRLPs on COX-2 mRNA expression The effect of CRLPs of varying fatty acid composition on expression of COX-2, an ... effect on the secretion of TGFb at any of the time points assessed (Fig 3E) The abundance of mRNA transcripts for each of the cytokines was determined after incubation of THP-1 macrophages with ... isoforms of IjB exist, the most predominant is IjBa [15] Phosphorylation of IjB by upstream kinases results in its Lys48-linked polyubiquitylation and degradation, permitting translocation of...

Ngày tải lên: 23/03/2014, 04:20

... of nuclear receptors (NRs) and cofactors, and then summarize the study of arthropod ecdysteroid receptors, including sequences, functions, ligand-binding characteristics and the application of ... recognized by other receptors For example, vertebrate steroid hormone receptors (such as GRs, mineralcorticoid receptors, progesterone receptors and androgen Arthropod nuclear receptors receptors) bind ... the OH group of the tyrosine amino acid residue are observed in three EcRs (Tyr408 of HvEcR, Tyr296 of BtEcR and Tyr427 of TcEcR), illustrating conservation of binding characteristics of the molting...

Ngày tải lên: 23/03/2014, 04:20