mechanism s involved in the deleterious effect of salt

... equilibrium analysis of the same samples s (bottom panel) show absorbance at 280 nm The solid line indicates best fit to a self-associating system The upper panel shows the residuals for the best fit 1.1 ... d2-crystallin Further increases in GdnHCl concentration induced unfolding of the dissociated monomers This unfolding process was investigated by quenching of intrinsic protein ANS is a sensitive probe ... further increased The unfolding curve using the ANS fluorescence probe suggested a multistate process, consistent with the results obtained by protein intrinsic fluorescence and CD The binding...

Ngày tải lên: 08/03/2014, 08:20

... N-terminal cysteine residues in the subunit of VnfA bind the single Fe -S cluster A possible scenario is that two of three cysteine residues (Cys15 and Cys8 or Cys10) bind the Fe -S cluster and the ... same Fe -S cluster because the amino acid sequence, Cys8Glu9Cys10, restricts the cysteine residues from binding to the same cluster Discussion Prosthetic group of VnfA The EPR data of the whole ... in vivo b-gal activity assays for cysteine variants of Strep-VnfA indicate that the N-terminal cysteine residues are plausible candidates for the ligands of the Fe -S cluster Thus, the locus of...

Ngày tải lên: 15/03/2014, 09:20

... values of the chimeric proteins were of the same order of magnitude as those of NADP-ME2 (Table 1) These results indicate that, despite the differences detected, the binding sites for the substrates ... the protein supports the hypothesis of the existence of an allosteric site responsible for such regulation, as in the case of maize photosynthetic NADP-ME [6] In agreement with this, the kinetic ... measured in the absence of the metabolites for each of the respective enzyme constructs The assays were performed at least in triplicate and the error bars indicate SD Significant inhibition (as indicated...

Ngày tải lên: 23/03/2014, 04:20

... region of the NOX1 gene contained a consensus MEF2-binding site that confers the responsiveness to PGF2a; (c) stimulation with PGF2a enhanced the binding of MEF2 to its consensus binding site in the ... production in vascular smooth muscle cells by inducing the expression of the NOX1 gene Results Determination of the 5¢-end of the NOX1 mRNA expressed in VSMC We reported the expression of novel ... level of the NOX1 transcript is much lower than the levels of other NOX isoforms expressed in vascular tissue, whereas inducible expression of NOX1 has been documented in association with various...

Ngày tải lên: 30/03/2014, 03:20

... translation initiation signal, as implemented in the GENSCAN analysis tool [28] Expression of fucokinase in mammalian cells The two splice variants of the murine fucokinase genes were expressed in ... ATG, suggesting that this segment is part of the CDS In conclusion, we propose that the CDS starts not at the third but at the first ATG in the murine sequence, and that the human CDS starts at the ... variant of L-fucokinase consisted of 3270 bp, encoding a protein of 1090 amino acids The sequence of the shorter cDNA was similar to the sequence of the IMAGE clone 4190449, consisting of 3057 bp The...

Ngày tải lên: 30/03/2014, 13:20

... provide insight regarding the mechanism, the substrate binding site and the role of the metal cofactor in the enzyme Results Cloning, expression and purification of BphH The bphH gene was inserted into ... stabilization of the anion by the electron withdrawing substituent This is in line with previous proposal of a step-wise hydration mechanism in the HPDA hydratases [7] Microorganisms that possess the meta-cleavage ... predominant in uence on catalysis rather than substrate binding Substrate specificity of BphH Substrate specificity of Mg2+ saturated BphH was determined by steady state kinetic analysis As HPDAs are...

Ngày tải lên: 30/03/2014, 15:20

... Swiss-Prot) are in bold The numbering of the yeast subunit e begins at the initiating methionine The star indicates the position of the unique cysteine residue of the yeast subunit e (position 28) The putative ... segment of subunit 4, whereas subunit e was still present On the basis of cross-linking data, the absence of subunit g in the latter mutant was attributed to the loss of interaction between subunits ... performed in the presence of NEM instead of CuCl2 Cross-linking conditions are described in the experimental procedure After dissociation of samples with SDS in the presence of 20 mM of NEM, aliquots...

Ngày tải lên: 31/03/2014, 01:20

... sister She never behaves with respect to the other Just because she is her sister – except perhaps in those cases when her mother says, “Give in to her.” In the game of sisters playing at “sisters,” ... are sisters: they are dressed alike, they walk about holding hands – in short, they enact whatever emphasizes their relationship as sisters visa-vis adults and strangers The elder, holding the ... the other sister in the whole play situation.) Only actions that fit these rules are acceptable in the play situation In the game a situation is chosen that stresses the fact that these girls...

Ngày tải lên: 23/06/2014, 09:52

... the business process of an organization According to [12], it can show the structure of the business process” which is lost when using “current process modeling” This is one of the reasons why ... processes of the organization while RAD technique links these business processes to the software development concepts (requirements, use case) Besides, using this framework, the business processes can ... in the IAM, the PM shows the casual and conditional relationships between different steps of the related transactions Capturing the business processes of the organization, it can be used as the...

Ngày tải lên: 02/08/2015, 13:22

... must satisfy restrictions of the null hypothesis, so the Wald statistics should be close to zero Table represents results for Wald tests of both hypotheses testing The p-value (p

Ngày tải lên: 30/03/2014, 05:20

... The mechanisms underlying the suppression by DCs of the expansion and differentiation of effector T cells and promotion of T-cell tolerance remain elusive One regulatory mechanism of DCs is the ... examined the change in the expression of IDO in CD11c+ DCs of Peyer 's patches after repeated oral administration of CII and subsequent induction of CIA In freshly isolated Peyer 's patches, the ... cell sorting system The expression of IDO mRNA was analyzed using RT-PCR β2-Actin was used as an internal control Each value is the mean ± standard deviation of replicate determinations in one of...

Ngày tải lên: 09/08/2014, 10:22

... analysis by plotting the specific binding of the radioligand on the X-axis and bound/free on the Y-axis The maximal binding is a measure of the total number of receptors present in the tissue ... 0.2% ascorbic acid) was infused into the right Substantia nigra Pars compacta (SNpc) at a flow rate of 0.2 μl/min After stopping the infusion of the toxin, the probe was kept in the same position ... couple positively to phospholipase C via guanine nucleotide binding proteins (G-proteins) whereby they stimulate phosphoinositide hydrolysis generating a second messenger cascade consisting of diacylglycerol...

Ngày tải lên: 10/08/2014, 05:21

... Patterns in the professional contacts of health professionals involved in ParkinsonNet may influence clinical processes and outcomes in several ways Specifically, professional contacts may improve the ... patients was based on consensus among the clinical authors of this paper We hypothesized that health professionals treating many Parkinson patients and health professionals in specialized hospital settings ... care professionals yielded lower values for most network measures compared to professionals based in hospital settings We conclude that the analysis of the network of health professionals showed...

Ngày tải lên: 10/08/2014, 11:20

... medium (SMGM), which consisted of smooth muscle basal medium (SMBM; M231; Cascade Biologics) and smooth muscle growth supplement (SMGS; Cascade Biologics) The final concentration of SMGS contained ... from PASMC by using TRIzol reagent (Sigma-Aldrich St Louis, MO, USA) according to manufacturer 's instructions RNA was reverse-transcribed to synthesize first-strand cDNA The specific primers were ... expression in human PASMC (e) Anti-proliferative effects of sildenafil is related to the SOC/Ca2+/NFAT pathway PAH is a disease of progressive vascular remodeling of the small pulmonary arteries...

Ngày tải lên: 12/08/2014, 14:20

... key tool in understanding the status and functions of the CNS, and investigating the mechanisms of chronic pain The importance of biological markers for assessing the extent of chronic pain or predicting ... treatment of chronic pain The change in concentration of various proteins or peptides in the CSF is one of the most sensitive indicators of pathology in the CNS The differing concentrations of various ... CSF, being in direct contact with the ventricles of the brain and the spinal cord, contains substances secreted from nerve cells, reflecting changes occurring in the CNS CSF analysis remains...

Ngày tải lên: 02/10/2015, 17:15

... reduce the effectiveness of these drugs in reducing anxiety and may increase drowsiness and the risk of side effects such as drowsiness St John s wort may reduce blood levels of cyclosporine, making ... episodes Ginseng taken with anticoagulants or with aspirin or other NSAIDs may increase the risk of bleeding Ginseng may intensify the effects of these drugs, causing an excessive decrease in ... potassium-sparing diuretics, such as spironolactone, making these diuretics less effective Licorice may intensify the effects of these drugs and increase the risk of side effects, such as headache, tremors,...

Ngày tải lên: 25/10/2013, 05:20

... tted assuming single phase kinetics (single phase dissociation/ association) The kinetic parameters were calculated from these ts using the BIAEVALUATION software (v2.1, BiaCore) ể FEBS 2004 ... changes necessary to the binding of PRK In agreement with this last hypothesis, our analysis of the kinetic properties of the GAPDHCP12 subcomplexes obtained with the K128A/E and R197A mutants showed ... and essential for the assembly of the GAPDHCP12PRK complex Our results also seem to point out that the S loop, known to be involved in the cofactor-binding site, may also be essential for the interaction...

Ngày tải lên: 19/02/2014, 16:20

... Restriction of the cavity size thus seems to be a major cause of discrimination against large substrates in PDCs Yeast PDCs not follow this substitution pattern as the basis of discrimination towards large ... of the substrate binding site is further supported by mutational studies of ZmPDC, because residue substitutions at the positions corresponding to 467 and 471 in IPDC in uence substrate binding ... these positions also in all plant PDCs A change in substrate specificity from pyruvate to indolepyruvate thus involves at least substitution of three residues in the substrate binding pocket In...

Ngày tải lên: 20/02/2014, 11:20

... type III PKSs, which lack this domain, is scant This domain promotes the binding of the acyl-CoA initiation unit to the ketosynthetase domain of the PKSs for polyketide biosynthesis [35] Therefore, ... antibioticus genes implicated in oleandomycin biosynthesis [40] Gene analyses and enzymatic assays suggest that the biosynthetic pathways of the aminonucleoside moieties of A201A and puromycin start ... from the pur cluster [6] This finding suggests that, similarly to the pur cluster, next to this ORF there should be additional genes encoding other proteins implicated in the biosynthesis of the...

Ngày tải lên: 21/02/2014, 01:21

... final stage of starch synthesis in the absence of concomitant amylopectin synthesis (reviewed in [41]) In vitro synthesis of amylose Because in Chlamydomonas amylose synthesis remains active when ... amylopectin synthesis machinery This system consists of intact starch granules purified from Chlamydomonas strains under physiological conditions where the synthesis of amylose has remained minimal These ... of storage starch synthesis show that amylopectin and amylose synthesis are partly disconnected and that amylose synthesis persists when the rate of polysaccharide and amylopectin synthesis become...

Ngày tải lên: 22/02/2014, 07:20