mda proline and enzyme activity

... markedly affected by temperature (Table 1) MDA and proline contents and enzyme activity Temperature influenced the MDA and proline contents in leaves The foliar MDA content was significantly higher ... read at 663 nm and 645 nm, respectively The chlorophyll a and b contents were calculated according to the method of Porra [28] Measurements of MDA, proline, and enzyme activity MDA in leaves was ... simulated climatic warming on SOD and CAT activities, contents of MDA, proline, carbohydrates and chlorophyll of Arabidopsis thaliana leaves, and leaf microstructure and ultrastructure, apart from...

Ngày tải lên: 11/08/2014, 11:21

... diabetes, and cardiovascular disease Diabetes 2007; 5: 1198-209 Hu XB, Zhang YJ, Zhang HT, et al Cloning and expression of adiponectin and its globular domain, and measurement of the biological activity ... adiponectin and the C-terminus of GLP-1-A Compared with native GLP-1, the fusion protein has a modified site and larger molecular size, and may circumvent the rapid inactivation and renal clearance ... sufficiently subtle to retain the biological activity of GLP-1 [7] Moreover, GLP-1 is a peptide with relatively low molecular weight and small molecular size, and most of them may not connect with plasma...

Ngày tải lên: 25/10/2012, 11:10

... assays (EMSA) and RdRp assays, and examined the influence of different cis-acting elements on binding of NS5B protein and RNA synthesis in vitro Materials and methods Expression and purification ... nucleotide fragment adjacent to the minus-strand 3¢ UTR), –RNAr2 (a random sequence corresponding to positions 2033–2500 of the minus-strand genome) and minus-strand 5¢ UTR Similarly, we found that the ... NS5B-binding sequence on the plusstrand 3¢ UTR or minus-strand 3¢ UTR (A) Various RNA templates containing the wild-type and the mutant plus-strand 3¢ UTR or minusstrand 3¢ UTR )RNA1 to )RNA5 represent,...

Ngày tải lên: 19/02/2014, 16:20

... proteolytic/thermal treatment of BLG at relatively high enzyme/ BLG ratios (1 : 10 and : 20) and at the highest temperature compatible with retention of enzyme activity and with the reversibility of structural ... 37 °C and pH 8.1 and the same amounts of enzyme present in the heated mixtures We found 25% and 35% inhibition of trypsin activity on 0.5 mM BAPA when native BLG was added at 0.075 mM and 0.15 ... fragments CP and 2, as well as fragments TP and 2, were further purified by RP-HPLC and SE-HPLC in the same conditions and with the same equipment used for analysis of the whole proteolyzate, and these...

Ngày tải lên: 21/02/2014, 15:20

... yeast cytochrome b and studied the effects on complex assembly and activity Secondary suppressor mutations arising from the original mutation were also identified collected, and the decylubiquinol ... cyanide, 30 lM equine cytochrome c and 0.025% (w/v) lauryl maltoside, pH 7.5 The concentration of bc1 complex in the activity assay was 2.5 nM (A) Activity of the mutant enzyme as a function of the ... further inhibition of the enzyme activity An altered protein– protein interaction between the ISP hinge region and the cytochrome b extramembranous cd2 helix may result in enzyme instability In R...

Ngày tải lên: 07/03/2014, 15:20

... Benchmark Protein Ladder (Gibco, Grand Island, NY, USA) the supernatant (SP) were resolved by SDS ⁄ PAGE and visualized by silver staining (Fig 3A) The IP and SP samples obtained with pre-immune ... and proteins from the supernatant fraction (SP) were resolved by SDS ⁄ PAGE and visualized by silver staining (D) Western blot analysis of IP and SP samples, obtained with pre-immune serum, and ... sodium carbonate and 0.05% formaldehyde Staining was stopped in 50% ethanol and 12% acetic acid for For western blot analysis, IP and SP samples were separated on a 13% SDS ⁄ PAGE gel and transferred...

Ngày tải lên: 15/03/2014, 23:20

... investigated using enzymes involved in the antioxydant defence, i.e GPOD, SOD, and in the intermediary metabolism, i.e ME, ICDH, G6PDH and GDH Materials and methods Plant growth and plant samples ... (Normapur), except Cd(NO3 )2 and HNO3 purchased from Merck (Pro Analysi) Enzyme assays and isoenzymes determination Frozen material (1 g FW) was homogenized with an ice-cooled mortar and ground in mL of ... was then maintained Protein contents Enzyme activities and isoenzyme analysis The total soluble protein content was examined in leaf and root samples Roots and L4 showed no significant changes...

Ngày tải lên: 15/03/2014, 23:22

... americanus [9] than to the mandibular organ-inhibiting hormones of the crab Cancer pagarus [10] MeMIH-A and MeMIH-B are non-sex-specific and are expressed in the eyestalks of males and females The expression ... membrane of the periopod and returned to the culture tanks At 24, 48 and 72 h after injection, the hepatopancreas and ovary of the shrimp were dissected for total RNA preparation, and the hemolymph ... describes the production of recombinant protein and dsRNA for reproductionrelated eyestalk neuropeptide gene, and use of an in vitro explant culture system and an RNAi technique to demonstrate the reproductive...

Ngày tải lên: 16/03/2014, 05:20

... encoding a fibrinogen-like protein, and also one for a cytokine, are strongly expressed Materials and methods Chemicals and enzymes The sources of chemicals and enzymes used were as given previously ... AB024737.1 and TECL5B_TACTR; AB024738.1) (iii) Angiopoietin: angiopoietin and precursors from mouse (AGP1_MOUSE; O08538 and AGP2_MOUSE; O35608), bovine (AGP1_BOVIN; O18920 and AGP2_BOVIN; O77802), and ... for EGF and required for biological activity [77], are also found in the sponge and are highly conserved in domain Likewise, amino acids, which are involved in ligand-receptor binding [52] and required...

Ngày tải lên: 16/03/2014, 16:20

... [4], and confirmed the following two-step enzyme activity: E þ G13 ! E-Gx þ G13Àx ð1Þ E-Gx þ G13 ! E þ G13þx ð2Þ Fig Expression and purification of sGas1523 and the mutant forms sGas1E161Q and sGas1E262Q ... Endo-b-(1,3)-glucanase activity was assayed by a reducing sugar assay [4] and b-(1,3)-glucanosyltransferase activity by an assay described in the Materials and methods No endob(1,3)-glucanase activity was ... concentrations of 0.02% (w/v) and 0.1 M, respectively, prior to division into two aliquots and the addition of the enzyme To test the effect of removal of N-linked chains on enzyme activity, % 20 lg of...

Ngày tải lên: 16/03/2014, 18:20

... stains and cultures, two blood cultures, and urinary antigen assays to detect Legionella pneumophila and Streptococcus pneumoniae All participants had a complete physical examination, and blood ... bacterial CAP and the small number of study subjects should be considered when generalizing the results using CRP and PCT to determine pulmonary TB and bacterial CAP In conclusion, serum CRP and PCT ... samples were obtained for measuring CRP and PCT before starting treatment Additionally, demographic data, a white blood cell (WBC) count and differential, and the Pneumonia Patient Outcomes Research...

Ngày tải lên: 22/03/2014, 18:20

... structure of the proteins, we analyzed the UV CD spectra of MS2 and VLPs in the absence and presence of 5.0 m and 9.0 m urea and 3.5 m and 5.0 m GdnHCl, the concentrations at which the greatest difference ... protein dimers and their selfassembly into an icosahedral capsid, while selectively encapsulating a single copy of viral plus-strand RNA by the coat, and ignoring viral minus strands and host nucleic ... urea, GdnHCl and high pressure on the structure and stability of whole particles (bacteriophage MS2 and VLPs) and assembly-defective coat protein species [coat protein dimers (dlFG) and single-chain...

Ngày tải lên: 30/03/2014, 11:20

... purifying an enzyme, a single major band on a gel would suggest a pure enzyme However this band could still be a contaminant; the enzyme could be present as a weaker (even nonstaining) band on the ... two alternative protein standards, bovine serum albumin and ovalbumin, are 0.66 and 0.75, respectively Plastic and glassware used in the assay should be absolutely clean and detergent-free Quartz ... nonstaining) band on the same gel Only by showing that the major band had enzyme activity would you be convinced that this band corresponded to your enzyme The method described here is based on the gel system...

Ngày tải lên: 10/04/2014, 11:03

... K L., Glish, G L., and McLuckey, S A (1988) Mass Spectrometry/Mass Spectrometry Technzques and Applications of Tandem Mass Spectrometry VCH, New York 16 Hogeland, K E , Jr and Demzer, M L (1994) ... used m protein chemistry m terms of senstttvtty and acceptance of solvents, detergents, and buffers, They must be able to handle impure samples and complex mixtures Last, but not least, the information ... Biemann, K and Scoble, H A (1987) Characterization by tandem mass spectrometry Science 231,992-998 18 Gtbson, B W and Cohen, P (1990) Ltquid secondary ion mass spectrometry of phosphorylated and sulfated...

Ngày tải lên: 11/04/2014, 10:11

... Virology Journal 2007, 4:73 On the other hand, both the G7L and A17L gene products, VV membrane proteins, are required for virus replication and are involved in the early development of IV ... IV and IMV membranes [13,14,5] A17L (p21K) and its cleavage product (21K) co-localized with GTPase Rab1, a marker of intermediate compartment (IC) membranes, the origin of viral membrane [15] and ... recruits and organizes the first recognized membrane and induces the correct formation of viral genome content through the proteolysis of viral core/membrane proteins However, the essentiality and...

Ngày tải lên: 18/06/2014, 18:20

... liver [25], brain [26] and eye [27] Recently developed rAAV vectors including new serotypes of AAV, mutants AAV and double stranded AAV have provided more opportunities and challenges for their ... for 10-20 depending on tissue size and then checked manually for pliability Sections μm thick were cut and stained with hematoxylin and eosin according to standard methods Histological evaluation ... received saline injection ELISA for the Detection of Serum hAAT and BAFF Levels and Antibodies against hAAT, bCII and mCII Detection of hAAT and anti-hAAT antibodies in mouse serum was performed as...

Ngày tải lên: 18/06/2014, 19:20

... Virology Journal 2007, 4:73 On the other hand, both the G7L and A17L gene products, VV membrane proteins, are required for virus replication and are involved in the early development of IV ... IV and IMV membranes [13,14,5] A17L (p21K) and its cleavage product (21K) co-localized with GTPase Rab1, a marker of intermediate compartment (IC) membranes, the origin of viral membrane [15] and ... recruits and organizes the first recognized membrane and induces the correct formation of viral genome content through the proteolysis of viral core/membrane proteins However, the essentiality and...

Ngày tải lên: 20/06/2014, 01:20

... bacterial strains, enzymes, and maize genotypes The cloning vectors pBluescriptSK+ (pSK+; Stratagene, La Jolla, CA) and pUC19 (Stratagene), and the RecAEscherichia coli strains DH5α and JM109 were ... PstI and partially digested with NcoI Fragments of both plasmids approximately 0.33 kbp, 0.69 kbp, and 4.4 kbp in size (respectively referred to as K1, K2, and K3 for pKNco and S1, S2, and S3 ... nucleus and facilitates nuclear transport of viral DNA [12] which may be analogous to the nuclear localization and/ or shuttling functions performed by begomovirus CPs and/ or NSPs [41,42]; and MSV...

Ngày tải lên: 20/06/2014, 01:20

... the progression of atherosclerosis Lipoprotein and CRP levels were measured at baseline and follow-up From the Cleveland Clinic Foundation, Cleveland (S.E.N., E.M.T., P.S., T.C.); Pfizer, New ... (C.O.); and Brigham and Women’s Hospital, Boston (P.G.) Address reprint requests to Dr Nissen at the Department of Cardiovascular Medicine, Cleveland Clinic Foundation, 9500 Euclid Ave., Cleveland, ... of atherogenic lipoproteins, CRP, and HDL cholesterol and the rate of progres- Table Baseline and Follow-up Values for Intravascular Ultrasonographic End Points and Change in Values from Baseline.*...

Ngày tải lên: 27/06/2014, 00:20