isolation and preparation of rna from rat blood and lymphatic microvessels for use in microarray analysis
báo cáo hóa học:" Validation of a flow cytometry based chemokine internalization assay for use in evaluating the pharmacodynamic response to a receptor antagonist" potx
... of the assay to demonstrate inhibition of ligand internalization at low concentrations of CCR2 inhibitor, was determined by serially diluting the CCR2 antagonist into whole blood and incubating ... concentrations of inhibitor, was determined by serially diluting CCR2 into whole blood and determining the concentration at which the assay %CV of inhibition became greater than 20% and using the ... whole blood alexa-488 labeled MCP-1 internalization assay was validated here for use in clinical trials investigating a CCR2 antagonist by examining a standard reagent concentration to use, the...
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Báo cáo khoa học: Structural characterization of N-linked oligosaccharides of laminin from rat kidney: changes during diabetes and modulation by dietary fiber and butyric acid pdf
... in the presence of protease inhibitors for further studies SDS/PAGE of laminin The purity of laminin was ascertained by 3.5% SDS ⁄ PAGE and visualized by silver staining for bands corresponding ... step, laminin from rat kidneys from different experimental groups was purified and purity was ascertained by SDS ⁄ PAGE using silver nitrate staining (depicted in Fig S1 for laminin from starch-fed ... weightÆday)1 in drinking water Isolation of laminin from kidney cortex Laminin from kidney was isolated according to Paulsson et al [23] Kidneys from individual groups were pooled FEBS Journal 278...
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Báo cáo y học: " Co-receptor usage and prediction of v3 genotyping algorithms in hiv-1 subtype b’ from paid blood donors experienced anti-retroviral therapy in chinese central province" pps
... Discussion In 1993, HIV-1 infection of paid blood donors in the central Chinese province of Henan and Anhui provinces constitutes a major epidemic in China[15] In September 2003, the “Four Frees and ... usage in clinical isolates Page of HIV-1 isolation from patients’ PBMCs Primary HIV-1 strains were isolated by co-culturing PBMCs from infected individual and those from two or more from healthy individuals ... Sequence analysis of gag and env genes of HIV type circulating in former blood donors of Fuyang city, Anhui province National medical journal of china 2007, 87(22) Wu Z, Liu Z, Detels R: HIV-1 infection...
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báo cáo hóa học: " Development and validation of a preference based measure derived from the Cambridge Pulmonary Hypertension Outcome Review (CAMPHOR) for use in cost utility analyses" doc
... endothelin receptor antagonists through to nebulised or continuous intravenous or sub-cutaneous infusions of prostaglandin or prostaglandin analogues [2] Many of these treatments are inconvenient ... into a preference based measure The approach used in this paper has been developed in Sheffield and used in the construction of the SF-6D and King's Health Questionnaire [9,17] An advantage of ... of serious illness Experience of serious illness in family Experience of serious illness in caring for others Main activity Education after minimum school leaving age Degree or equivalent professional...
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Báo cáo y học: "Sales of antidepressants, suicides and hospital admissions for depression in Veneto Region, Italy, from 2000 to 2005: an ecological study" doc
... for depression and suicide rates, for both sexes Our final finding is that overall suicide rates remained unchanged during the period under examination, in spite of the observed exponential increase ... shown in Table Overall, the admissions for depression declined both in males and in females from 2000 to 2005 The number of beds in the Veneto region changed from a maximum of 796 in 2000 to a minimum ... prescribing trend, we find that in the 0-14 age band AD consumption steadily increased between 2000 and 2005, both in males and in females Looking at the 1564 age band, we find that AD prescription increased...
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DEVELOPMENT OF THE OZONIZER AND OZONATION TECHNOLOGY FOR WATERWORKS IN JAPAN
... status of each of the issues RESEARCH AND DEVELOPMENT IN INCREASING OZONE CONCENTRATION Ozone generation Method and scale of use Table summarizes the ozone generation method and their scales of use ... result in an increase in ozone - 38 - Journal of Water and Environment Technology, Vol.2, No.2, 2004 concentration However, at rates of up to 160 watts min/L, ozone concentration increases linearly ... Mechanism for bromate formation - 42 - Journal of Water and Environment Technology, Vol.2, No.2, 2004 New water quality standards for drinking water to be enforced in April 2004 indicates a standards...
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Development of a Regional Risk Management Framework for APEC Economies for use in the Control and Prevention of Introduced Marine Pests
... Within APEC little is known on the introduction of marine pests Little practical information on measures to prevent introductions Lack of knowledge in what individual countries are doing ... water and hull fouling are the most important vectors International shipping, aquaculture and biodiversity are most threatened values Amount of commercial shipping and number of trading partners ... enhance the effectiveness of existing instruments within APEC Institutional arrangements for managing the marine environment is fragmented in most economies Baseline surveys to identify IMP...
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(1) how learners approach learning, both in and out of classrooms, and (2) the kinds of strategies and cognitive processing they use in second language acquisition
... intelligently strategies B Overcoming limitations speaking and writing I Metacognitive INDIRECT STRATEGIES Strategies in A Centering your learning B Arranging and planning your learning 23 C Evaluating your ... affect students’ use of learning strategies Generally speaking, students majoring in humanities used more and a wider range of strategies than those majoring in science/engineering in several studies ... differences in L2 learning strategies Regarding the general use of language learning strategies, Green and Oxford (1995) reveal that 15 out of 50 strategies on the Strategy Inventory for Language Learning...
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Use of Alternative Fuels in Cement Manufacture: Analysis of Fuel Characteristics and Feasibility for Use in the Chinese Cement Sector pdf
... landfilling and other forms of thermal combustion In comparison to other incineration processes for energy capture, end use in cement manufacturing has the key benefits of utilizing pre-existing ... the challenge of disposing of incineration ash since it can be incorporated into clinker Landfilling plastic ash is often prohibited because the embodied heavy metals can leach and pose a threat ... equivalent in coal Thus the lower cost of fuel can offset the cost of installing new equipment for handling the alternative fuels Energy normally accounts for 30-40% of the operating costs of cement...
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Báo cáo hóa học: "Safety evaluation of allogeneic umbilical cord blood mononuclear cell therapy for degenerative conditions" pot
... relevant information about the initial culture is entered in the batch information record including test results for sterility, mycoplasma and endotoxin Cell growth was regularly monitored and the inspection ... technique and consent forms were obtained from each patient before initiation of treatment Cell processing Umbilical Cord Blood (100~ 150 mL) was collected from healthy unrelated donors (signed an informed ... data analysis The SPSS 13.0 statistical package was applied for statistical analysis Results Administration of cord blood mononuclear cells via intrathecal and intravenous routes was well tolerated...
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Báo cáo hóa học: "Research Article Design of a Versatile and Low Cost μVolt Level A to D Conversion System for Use in Medical Instrumentation Applicatio" pdf
... series of measurements were taken Data logging over a number of minute intervals while toggling the light source on and off for periods of minute resulted in a series of graphs of the type shown in ... effective in providing direct readout of high intensity photon beams generated by clinical linear accelerators in situations involving high levels of background radiation and interference and where remote ... portable battery-operated instruments, in addition to its potential for incorporation into laboratory instrumentation where the effects of high levels of environmental noise and interference need...
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Báo cáo khoa học: " Evaluation of serum chondroitin sulfate and hyaluronan: biomarkers for osteoarthritis in canine hip dysplasia" pps
... clinically useful indices of the effects of isolated joint injury, the progression of the joint changes and the response to therapy The levels of the CS epitopes 3B3 and WF6 in serum were increased, ... [7] An increasing of IL-1 stimulates the production of collagenase, stromelysin and prostaglandin E2 by the chondrocytes This mechanism induces inflammation to proceed in the dysplatic joint [8,24] ... white blood cell count (WBC) and the platelet count Two milliliters of serum were analyzed for blood chemicals, including aspartate aminotransferase, alanine aminotranferase, blood urea nitrogen and...
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báo cáo khoa học: "Case report of rapidly progressive proliferative verrucous leukoplakia and a proposal for aetiology in mainland China" pps
... properties of PVL PVL is a rare and specific disease that differs from OVL, and is often seen in middle-aged and elderly women, occurring predominantly on the bucca, palate, gingiva, and tongue ... report of rapidly progressive proliferative verrucous leukoplakia and a proposal for aetiology in mainland China World Journal of Surgical Oncology 2011 9:26 Authors’ contributions GL and WY ... epidemiological investigation in China PVL is usually chronic and progressive, and a patient often has a long history of leukoplakia before he/she attends a clinic [8,10] Most cases progress for 20-25...
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báo cáo khoa học:" Rasch analysis of the Hospital Anxiety and Depression Scale (HADS) for use in motor neurone disease" pot
... diagnostic interview These findings support the use of the modified HADS-D and HADS-A for use with patients with MND within clinics and research, and support the modified HADS-T for research use where ... performance of the HADS may vary by diagnostic group and reinforces the need for clinicians and researchers to formally test the psychometric properties of the instruments they intend to use ... HADS is commonly used in MND clinics and has been used in a number of past studies in MND [4-7] Rasch analysis of the scale makes an important contribution to current understanding of the measurement...
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báo cáo khoa học: " Transcriptomic identification of candidate genes involved in sunflower responses to chilling and salt stresses based on cDNA microarray analysis" pps
... stress per se In another study the expression of 150 genes in response to wounding and insect feeding was carried out [58], finding that some of the responding genes, for which the inducing stimulus ... (94°C) for min, followed by 32 cycles of 94°C for 30 sec; annealing temperatures were programmed at a descendant ramp from 60°C to 55°C and 72°C for 30 sec and a final extension of 72°C for minutes ... composed by four seedlings, were processed and evaluated independently for each stress treatment: chilling, salinity and control Amplification and preparation of cDNAs for microarrays construction...
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Báo cáo y học: "Abnormal networks of immune response-related molecules in bone marrow cells from patients with rheumatoid arthritis as revealed by DNA microarray analysis" ppsx
... usage, including disease-modifying agents and steroids, and laboratory testing, including rheumatoid factor (RF) and C-reactive protein (CRP), of the nine RA patients are shown in Table None of the ... first report to combine DNA microarray with bioinformatics for describing gene expression profiles from RA BM cells and for revealing abnormal networks involving immune response- and cell cycle-related ... statistical analysis, and drafted and revised the manuscript HS and CA assisted with the acquisition of data and analysis RS performed mRNA expression analysis with microarrays YS and KO treated and...
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Báo cáo y học: " Derivation of normal macrophages from human embryonic stem (hES) cells for applications in HIV gene therapy" pps
... against other invading pathogens and succumbs to opportunistic infections There is a great deal of progress in the area of stem cell gene therapy for AIDS [12] A primary goal of many ongoing ... peripheral blood, fetal liver, and cord blood [34] However, the number of cells that can be obtained for manipulations is not unlimited Therefore, deriving CD34 cells for therapeutic and investigative ... AI054907 We thank Julie Morris, Sarah Akkina and Jennifer Quick for help with maintaining hES cells and culturing embryoid bodies We thank Leila Remling for isolating fetal CD34 cells We thank 19 20...
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Báo cáo sinh học: "A dual function fusion protein of Herpes simplex virus type 1 thymidine kinase and firefly luciferase for noninvasive in vivo imaging of gene therapy in malignant glioma" ppsx
... introduced and increasingly used for temporal and spatial monitoring of transgene expression [11-13] Bioluminescence imaging (BLI) using luciferase (Luc) from the North American firefly Photinus ... implying that transfer and distribution of the transgene in human brain tumors were very low in vivo and differed obviously from the findings in animal experiments Presently, the standard method for ... Bioluminescence imaging of nude mice carrying HSV-TK-Luc expressing U87MG gliomas Bioluminescence imaging of nude mice carrying HSV-TK-Luc expressing U87MG gliomas (A) Serial images from a mouse...
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Development of ionic liquid based liquid phase microextraction, and zeolite imidazolate frameworks based sorbent phase based microextraction combined with chromatography for applications in environmental analysis
... preparation and preconcentration methods is necessary prior to instrumental analysis The main aim of sample preparation is to clean up, isolate and concentrate the analytes of interest, while rendering ... development of simplified and miniaturized methods for LLE was focused on small organic solvent drops in the sample preparation system LPME involves the use of a few microliters of extraction solvent for ... employed for isolation and preconcentration of analytes from aqueous samples The vast range of commercially available materials and those prepared in- house and the different configurations in which...
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Development of liquid phase microextraction techniques combined with chromatography and electrophoresis for applications in environmental analysis
... to determine the concentration of pollutants in the environment, is therefore very important Environmental analysis includes five steps: environmental sampling and handling, sample preparation, ... by a microsyringe and introduced to a GC for analysis However, the use of a Teflon rod was found to be inconvenient as the extraction and injection are performed separately Jeannot and Cantwell ... corresponding phenols and amines in the injection port or in the column during the analysis Therefore, derivatization of carbamate is desirable before GC analysis Different derivatization reactions including...
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