inverting the enantioselectivity of p450pyr monooxygenase by directed evolution

... view of the PDMS valves The thick grey and red layer is the PDMS chip with the features of the microfluidic channel Blue indicates the thin PDMS layer with the features of the valve B) When the ... representation of the microfluidic channels (A) the original microfluidic channel (B) the modified microfluidic channel (C) the connections and connectors needed for the original microfluidic channel (D) the ... set-up (A) the schematic representation of the multi-channel 3D-μFCCS, (B) the geometry of the 3D-μFCCS (the micropillar array separates the two side channels from the central channel) (C) the close-loop...

Ngày tải lên: 12/09/2015, 10:22

... space of chamber will be formed between the bottom of the carriers and the bottom of chambers, which is separated 33 with the main flow environment at the top of sandwich construct by the minusheet ... variations of natural ECMs and the transport barriers caused by the introduction of top ECM layer, which can slow down the exchange of nutrients, products, and chemical signals with the bulk of the ... drainage Fig 3.9 The excretory function quantified by the ratio of the area of fluorescein in intra-cellular sacs to the total area covered by cells viii Fig 4.1 XPS wide scanning spectrums of PET, PET-g-AAc,...

Ngày tải lên: 27/11/2015, 12:39

... TABLE OF CONTENTS Acknowledgement Part I: Introduction Rationale of the study Aims of the study .2 Scope of the study Methods of the study .3 Designs of the ... listening by considering anything that they will want to learn from the content of the audio segment Once they have written down or shared these ideas, then play the audio segment, allowing the students ... what they think will be the content of the segment Then, watch the segment again, this time with sound, allowing students to take notes if helpful for their skill level After the completion of the...

Ngày tải lên: 11/12/2013, 23:53

... Controller of HMSO The additional information is copyright of the Qualifications and Curriculum Authority By agreement between the Controller of HMSO and QCA reproduction of any of the material ... self, their unique potential, their understanding of their strengths and weaknesses, and their will to achieve As their curiosity about themselves and their place in the world increases, they ... see the mathematics programme of study for more information In English, the three sections of the programme of study each contain Breadth of study requirements In mathematics and science there...

Ngày tải lên: 02/04/2014, 05:20

... characteristics, the frequency and severity of attacks, the presence and degree of temporary disability, and the profile of associated symptoms such as nausea and vomiting The patient’s history of, response ... brain tumor As part of diagnosing migraine, the physician excludes any secondary causes of the patient’s headache In addition, the physician determines whether the patient has other coexisting primary ... management is often required since patient responses to these therapies are not always predictable Therefore, management is often indivudalized The choice of treatment should consider, among other characteristics,...

Ngày tải lên: 21/02/2014, 12:20

... [17: 2] The meaning of a sentence is not the sum of the meanings of the words used in the sentence It is more correct to regard it as the function of the meanings of the words used in the sentence, ... (written at the center of the letter, after the heading) The body (written inside the whole margin of the letter) The close (written at the right margin) The date (written at the right margin) The signature ... correspondence with the other great men in the state Bacon To facilitate correspondence between one part of London and another, was not originally one of the objects of the post office Macaulay The letters...

Ngày tải lên: 19/03/2014, 17:07

... close to the patient as the GP We are often not aware of that!” (1/60, GP6, m, 56 y)] Due to the lack of reimbursement and their high workload, they very often stated that they just not have the capacity ... promotion in the elderly One of the most important pre-conditions for the majority of the GPs to provide health promotion in the elderly was sufficient reimbursement by the healthcare system for the time ... benefit for the health or the quality of life of elderly patients Furthermore, the GPs stated that as long as there is no adequate reimbursement for health promotion in the elderly, they will just...

Ngày tải lên: 22/03/2014, 14:20

... and the inferences that can be based on our findings Further studies of the sources of endogenous oxalate synthesis in humans are needed The phenotypic features of Agxt) ⁄ ) mice are probably the ... available for fatty acid biosynthesis Taken together, these results seem to be indicative of an adaptation in favor of liver gluconeogenesis in response to the lack of AGT1 Other downregulated enzymes, ... only after the liver mitochondrial form of the AGT1 enzyme had been induced by glucagon [9] However, the peroxisomal form of 4770 SPT ⁄ AGT predominates during constitutive expression of rat and...

Ngày tải lên: 23/03/2014, 03:20

... induction of an oxidative burst was detected in BY- 2 cells treated with the AaGlucan (Fig 8B) As the AaGlucan induces the expression of tobacco BY- 2 chitinase (TBC)-1 in the BY- 2 cells, we further ... the STC gene remained expressed during the course of the experiment compared to the control No induction of the PR3 genes was observed For the analysis of the AaGlucan eliciting activity in tobacco ... synthesized as they are and secreted outside of the cells of the fungus, similarly to some cyclic glucans and nod-factors secreted by rhizobial bacteria [16,22] If so, the function of these b-glucan...

Ngày tải lên: 23/03/2014, 11:20

... depends on the way he assesses the importance of the components of each language Of 13 all the above definitions, the one that Richard and Platt (1992) proposes in the Dictionary of language ... terms of their level and their age range (most of them are years old) As the classes progressed, the children demonstrated that they knew more about the language to respond and act out the commands ... method Because of the lack of teaching aids, text book and the limited of creativeness in each lesson so the number of students who prefer studying the current method and the number of students...

Ngày tải lên: 26/03/2014, 00:16

... data was performed with the LightCycler software (version 3) Crossing points were assessed by the second derivate maximum algorithm and plotted against the concentrations of the standards Sample ... were cloned into the vector pCR2.1TOPO (Invitrogen, The Netherlands) A standard curve with dilutions of the appropriate plasmid in duplicates was included in each PCR run Analysis of RT-PCR expression ... patients gave written informed consent to participate in the study according to the Declaration of Helsinki The study was approved by the local ethics board Blood and bone marrow samples Bone...

Ngày tải lên: 18/06/2014, 16:20

... moving objects of interest in the scene The adaptive threshold is obtained by using δn, the recursive average of Δn, as a variable amplifying gain for the threshold (17) The increase of the threshold ... variations of the signal These two variables are used to define the upper and lower bounds between which the grey level of the signal should remains In order to take into account the variations of the ... enabling to check the whole coherence of the architecture for any conflicts during the parallel processing In order to take into account the impact of the nonidealities introduced by the analog parts...

Ngày tải lên: 21/06/2014, 07:20

... explaining the 93% of total variation of training data On the other hand, we are also interested in characterizing the temporal evolution of the action Therefore, we compute the main direction of the ... estimating the parameters of our 3D model from a sequence of 2D images In [5] the action model improved the efficiency of the tracking algorithm by constraining the space of possible solutions only to the ... the CMU database, which correspond to the markers placed on each side of the head Notice that our model considers the left and the right parts of the hip and the torso as a unique limb, and therefore...

Ngày tải lên: 21/06/2014, 19:20

... with the fixed ground plane The distance between them determines the width and the mean determines the position of the object The height (e.g., of a person) is calculated by intersecting the view ... the actual tracking confidence The idea is to adapt wisely, that is, the more confident the smart camera about actually tracking the target itself is, the less the risk of overlearning is and the ... horizon, the detected position lies behind the camera and it will be ignored The extracted data is then sent to the server along with the texture of the object 3.2.4 Parameter selection The goal...

Ngày tải lên: 22/06/2014, 22:20

... determining the apparent sound insulation (that perceived by the occupants) depends on of the construction details of the walls, the floors and their junctions This Guide is the derivative of four ... could add other significant paths that reduce the overall sound isolation Whether for transmission from the room above to the room below, or vice versa, the Apparent-STC is the same (and the same ... reduction of sound isolation due to flanking, but they are outside the scope of this guide The experimental study included only a limited set of constructions Specific constraints imposed on the research...

Ngày tải lên: 08/08/2014, 13:21

... whether sigma-1 receptors were involved in the mechanism underlying the beneficial effects of fluvoxamine against the postoperative delirium of these patients In order to confirm the role of ... hypnotics Therefore, we cannot exclude a possible contribution of these hypnotics on the efficacy of fluvoxamine for delirium A further study of fluvoxamine alone will be necessary Delirium is theorised ... would also be of interest In all the patients reported on here, low doses of flunitrazepam or lorazepam were used for the treatment of insomnia since these drugs are considered to be the most effective...

Ngày tải lên: 08/08/2014, 23:21

... with both the anti-VAP-1 mAb and the negative control mAb The specific VAP-1 value was calculated by subtracting the mean background value of the negative control from the mean value of VAP-1 ... overnight and then at 37° C for h The wells were washed six times with 0.1% Tween 20 in PBS (Tween/PBS) and then blocked to prevent non-specific adsorption by the addition of 200 μL of PBS containing ... because the levels of these adhesion molecules change in different states, the determination of their concentrations can be beneficial for the diagnosis and treatment and monitoring of diseases [9,12,16,17]...

Ngày tải lên: 09/08/2014, 03:22

... of the CTV + cm, including the Nasopharynx, quadratic mean of the SD of the distance of the delineation to the median surface as measured for each region Agreement depicts the percentage of the ... mean of the SD of the distance of the delineation to the median surface as measured for each region Agreement depicts the percentage of the surface for each region on the median surface, where the ... implementation of big brother software enabling analysis of the data, PN: observer, design of the study, FPam: Radiologist, interpretation of anatomical location of variation, design of the delineation...

Ngày tải lên: 09/08/2014, 08:22

... years) Six of these patients had prior pelvic radiation therapy The type of previous radiation was EBRT alone patient, EBRT plus brachytherapy patients and brachytherapy alone patients The median ... verification of applicator placement The median dose for intracavitary brachytherapy was 20 Gy (12 – 20 Gy) in 3–5 fractions prescribed at 0.5 cm from the surface of the applicator One of these patients ... respectively The cumulative doses were highest in these two patients No other significant grade or higher morbidity was noted Discussion Radiotherapy is the main therapeutic modality in the management of...

Ngày tải lên: 09/08/2014, 09:22

... relevant In the context of LBP, the use of IL-1 blockers is very attractive, although many questions need to be addressed before starting to use such therapy: what is the best route of administration, ... gene therapy study Arthritis Res Ther 2007, 9:R83 Le Maitre CL, Hoyland JA, Freemont AJ: The role of interleukin1 in the pathogenesis of human intervertebral disc degeneration Arthritis Res Ther ... in the structural damage process of OA but also plays an important role in pain transmission Results from in vitro studies and animal models of OA support the dominant role of IL-1β early in the...

Ngày tải lên: 09/08/2014, 10:21