enterotoxins stayphylococcal enterotoxin b and bioterrorism

... much lower rate Both MK591 and SB203580 were able to block SEB-induced cell proliferation (Table 2) Effect of SEB and 5-LO inhibitor MK591 on human renal epithelial proximal tubular cells (REPTC) ... inhibitors All inhibitors of the lipoxygenase pathway were able to block SEBinduced MAPK and p38kinase activation but only had a partial inhibitory effect on JNK activation (Table 1) MAPK inhibitor ... whereas JNK was quantitated using both kinase and immune-assays; 0, 1, 5, 30 and 60 refer to the time (min) PBMCs were exposed to SEB 0, PBMCs not exposed to SEB but subjected all other experiment...

Ngày tải lên: 30/03/2014, 04:20

... Rodent Diet 5053 (LabDiet™) and water ad libitum Mice were housed in static cages with BedO’Cobs™ bedding inside BioBubble® Clean Rooms that provide H.E.P.A filtered air into the bubble environment ... reactivity and determine specificity We then subcloned and rescreened SEB-specific hybridomas by ELISA Three hybridomas, HuMAb-79G9, HuMAb-100C9 and HuMAb-154, met the binding (Figure 1) and selectivity ... these hybridomas by ELISA for reactivity to STEB, an SEB vaccine which is a recombinant and attenuated form of SEB [9] STEB contains site mutations in the hydrophobic binding loop, polar binding...

Ngày tải lên: 11/08/2014, 08:21

... Isopropylthio--D-galactoside Kb Kilobase LB Luria and Bertani PBS Phosphate buffer saline PCR Polymerase Chain Reaction S aureus Staphylococcus aureus SDS Sodium dodecyl sulfate SEB Staphylococcal enterotoxin < /b> B SEs ... cấu trúc chiều protein SEB (Nguồn : http//www.pdb.org/pdb/explore.do?structureId=3SEB ) 1.3.2 Cơ chế gây độc staphylococcal enterotoxin < /b> B Staphylococcal enterotoxin < /b> B (SEB) trung gian kích thích ... tế b o vi khuẩn Escherichia coli DH5α Nguyên lý: Biến nạp trình chuyển ADN trực tiếp vào tế b o nhận, tế b o có khả tiếp nhận ADN gọi tế b o khả biến Quá trình gồm giai đoạn: tạo tế b o khả biến...

Ngày tải lên: 12/11/2012, 18:00

... thống [Thị trường b n lẻ Việt Nam: Đa dạng tiềm năng] http://vnbusiness.vn/articles/th%E1%BB% 8B- tr%C6 %B0 %E1%BB%9Dng -b% C3%A1n-l%E1%BA%BBvi%E1%BB%87t-nam-%C4%91-d%E1%BA%A1ng-ti%E1%BB%81m-n%C4%83ng/ ... Hình 2.1: Sơ đồ b trí quầy hàng cửa hàng Citimart Sommerset b Đối vớichi nhánh Citimart B& B, NguyễnLương B ng, quận Tầngtrệt Hình 2.2: Sơ đồ b trí quầy hàng cửa hàng Citimart B& B Green View Tầng ... Hình 2.3: Sơ đồ b trí quầy hàng cửa hàng Citimart B& BGreen View c Đối với chi nhánh Citimart B& B Nam Long, HàHuyTập, quận Tầng Hình 2.4: Sơ đồ b trí quầy hàng cửa hàng Citimart B& B Nam Long Tầng...

Ngày tải lên: 01/04/2013, 11:40

... friend a lover b lubber 19 The symbol of peace is the _ a dove b dub B and V Minimal Pair Quiz Click the answer button to see the answer There is a worldwide _ on ivory trade a ban b van The _ ... live http://binhqx.violet.vn b lib 19 A style of very fast dancing is known as _ a jibe b jive 20 Why you always argue and _ about where we are going for vacation? a bicker b vicar B and V Minimal ... b libber 18 _ Shepherd was famous for her role in the TV series "Moonlighting" a Civil b Cybill 19 Park your car next to the _ a curve b curb 20 Taxis are also known as _ a calves b cabs B...

Ngày tải lên: 25/08/2013, 08:10

... (peptides 6a–d) were subjected to N-terminal sequence analysis (B) Sequences of ETA-A and ETA -B The A and B moieties are connected by both a peptide bond (Arg279-Gly280) and a disulfide bridge (Cys265–Cys287) ... intermediates 1, 4a, 4b, 4d, 6a and 6c; CPVAAGECA for intermediates 6b and 6d; and PALA for intermediate 4c Western blot analysis with an antibody against ETA showed that cathepsins B and D degraded ... induced by cathepsin B and ⁄ or D within the ETA sequence at various pH values (Fig 4A ,B) The proteolysis of ETA at pH or by cathepsin B and ⁄ or D was analyzed by reducing SDS ⁄ PAGE (Fig 4A), and...

Ngày tải lên: 18/02/2014, 04:20

... using mM Mes as a buffer Shown are stefin A and stefin B at pH and variant of stefin B and the P79S mutant of the variant at both pH and pH Each panel is a plot of heat change on ligand addition (kcalÆmole)1) ... also been obtained (not shown) (A) Stefin B wildtype (E31 isoform) at pH 5, 40 °C, and 50 lM Cu2+ in the buffer (B) Stefin B wildtype (E31 isoform) at pH 5, 10% TFE, 25 °C, and 50 lM Cu2+ in the buffer ... J 20, 4629– 4633 12 Stubbs MT, Laber B, Bode W, Huber R, Jerala R, ˚ ˇ ˇ Lenarcic B & Turk V (1990) The refined 2.4 A X-ray crystal structure of recombinant human stefin B in complex with the cysteine...

Ngày tải lên: 19/02/2014, 06:20

... Standard Book Number 0-309-XXXXX-X (Book) International Standard Book Number 0-309- XXXXX -X (PDF) Library of Congress Control Number: 00 XXXXXX Additional copies of this report are available ... populations, members of the public, and policy-makers There is insufficient understanding about the extent and seriousness of this public-health problem, so inadequate public resources are being allocated ... problems occurred with this vaccine in 2008 (adult and dialysis formulations of Recombivax HB) and 2009 (pediatric formulations of Recombivax HB and Pediatric Engerix -B) A shortage was avoided because...

Ngày tải lên: 06/03/2014, 01:20

... million HBV HCV HIV/AIDS About 21% About 75% About 65% 14,016 12,000 3,000 Deaths in 2006 Related to Infectiona ,b No No Yes VaccinePreventable Birth, blood, sex Birth, blood, sex Birth, blood, ... problems occurred with this vaccine in 2008 (adult and dialysis formulations of Recombivax HB) and 2009 (pediatric formulations of Recombivax HB and Pediatric Engerix -B) A shortage was avoided because ... grams and born to hepatiA tis B surface antigen-positive women should receive singlea ­ ntigen hepatitis B vaccine and hepatitis B immune globulin in the delivery room as soon as they are stable and...

Ngày tải lên: 06/03/2014, 01:20

... subunits Elongin B and elongin C bind stably to each other (elongin BC complex), and elongin A has the ability to bind to elongin C but cannot bind directly to elongin B Elongin B has a ubiquitin homology ... following four combinations: cerulean-elongin B and citrineelongin C; cerulean-elongin B and elongin C-citrine; elongin B- cerulean and elongin C-citrine; and elongin B- cerulean and citrine-elongin ... was observed A similar level of FRET signals could be detected in the combination of ceruleanelongin B and elongin C-citrine, as shown in Fig 1D and Table FRET between elongin B- cerulean and elongin...

Ngày tải lên: 16/03/2014, 05:20

... hepatitis B and hepatitis C are important public health problems and that there are several barriers to prevention and control efforts, such as a lack of knowledge and awareness about chronic ... National Institutes of Health, Bethesda, Maryland Randall R Mayer Chief, Bureau of HIV, STD, and Hepatitis, Iowa Department of Public Health, Des Moines, Iowa Margaret L Brandeau Professor, Department ... preventing hepatitis B and hepatitis C is the lack of knowledge and awareness about these diseases among health care providers, social service providers, and the public, especially among members of specific...

Ngày tải lên: 22/03/2014, 17:20

... modulated by the NF-jB subunits p65 and p50, and also by TNF-a Although p65 is probably expressed in the oocyte and the pre-implantation embryo, it remains to be shown if the TNF-a ⁄ NF-jB signaling ... element is targeted by the NF-jB proteins p50 and p65 It has been well established that jB sequences are targeted by the abundantly expressed NF-jB [13–15] Involvement of the NF-jB in gene regulation ... NF-jB proteins and TNF-a modulation was abolished when the F1MutkB jB mutant was similarly assayed (Fig 8B) , unequivocally 844 K. -B Choo et al demonstrating positive modulation by NF-jB and TNF-a...

Ngày tải lên: 29/03/2014, 00:20

... plastic bag and pour in the hybridization mix Gently wet the membrane, avoiding bubble formation Exclude air as much as possible before heat-sealing the bag 26 Lin Sandwich the bag between two ... hepatitis B virus (HBV) DNA have been used widely (Table 1), but their interpretation and significance have yet to be defined HBV DNA assays are limited by lack of standardization and variable sensitivity ... 600 bp signal being the brightest Filled and open triangles point to the 370 and 235 bp amplification products (Middle and Bottom Panels) Hybridization to the primer probes 1561+ and 1752+ Table...

Ngày tải lên: 11/04/2014, 09:45

... ELISAs have been developed for quantitation of DHBsAg (Fig 6) and detection of anti-DHBs (Fig 7A) and anti-DHBc (Fig 7B) antibodies In the DHBsAg ELISA rabbit anti-DHBs antibodies (see Subheading ... PBS and incubate with biotin-labeled anti-rabbit Ig for 30 Wash sections × in PBS and incubate with streptavidin-labeled HRP for 30 10 Wash sections × in PBS and develop with 0.5 mg/mL of DAB ... sulfate-precipitated rabbit anti-DHBsAg antibodies at a dilution of 1:500 in bicarbonate buffer, pH 9.6, and incubate at 37°C for h and then at 4°C until use Block wells with 200 ␮L of 5% SM-PBS/T Set up standard...

Ngày tải lên: 11/04/2014, 09:45

... sequences and the quantity of primers and probes used for the assay, as well as the amplification schedule, were described elsewhere [13,14] KRECs, TRECs, and TRAC copy number has been obtained by extrapolating ... copies/106 PBMC or copies/mL obtained respectively by multiplying the above calculated value by 106, or, as done by Chen et al [15], by the number of lymphocytes plus monocytes (which are the cells obtained ... PBMC preparation) Finally, the average number of B- cell divisions was evaluated, as reported by van Zelm et al [5], by calculating the difference between the cycle threshold number obtained by...

Ngày tải lên: 18/06/2014, 16:20

... PHA-680626 exhibits anti-proliferative and pro-apoptotic activity on Imatinib-resistant chronic myeloid leukemia cell lines and primary CD34+ cells by inhibition of both BcrAbl tyrosine kinase and Aurora ... Gontarewicz A, Balabanov S, Keller G, Colombo R, Graziano A, Pesenti E, Benten D, Bokemeyer C, Fiedler W, Moll J, Brummendorf TH: Simultaneous targeting of Aurora kinases and Bcr-Abl kinase by the small ... inhibits ABL, and aurora kinase inhibitors that also inhibit ABL can be considered a potential therapeutic alternative for patients resistant to Imatinib [35] Cell lines and tumors can often exhibit...

Ngày tải lên: 18/06/2014, 22:20

... were blocked with 3% BSA in PBS for h and incubated with rabbit anti-NF- B p65 antibodies Page of 12 (1:200, Santa Cruz Biotechnology, Santa Cruz) overnight at 4°C After washing, cells were incubated ... analyze NF- B (A) and AP-1 (B) DNA-binding activity by EMSA, as described in Methods Binding specificity was confirmed by unlabelled probe (100-fold in excess; lane 5) to compete with labelled oligonucleotide ... expression by ATL in LPS-stimulated BV-2 cells BV-2 cells were pretreated with ATL (1, 10 and 100 nM) for 30 in the absence or presence of 100 μM Boc-2 (30 before ATL treatment) followed by incubation...

Ngày tải lên: 19/06/2014, 22:20

... PHA-680626 exhibits anti-proliferative and pro-apoptotic activity on Imatinib-resistant chronic myeloid leukemia cell lines and primary CD34+ cells by inhibition of both BcrAbl tyrosine kinase and Aurora ... Gontarewicz A, Balabanov S, Keller G, Colombo R, Graziano A, Pesenti E, Benten D, Bokemeyer C, Fiedler W, Moll J, Brummendorf TH: Simultaneous targeting of Aurora kinases and Bcr-Abl kinase by the small ... inhibits ABL, and aurora kinase inhibitors that also inhibit ABL can be considered a potential therapeutic alternative for patients resistant to Imatinib [35] Cell lines and tumors can often exhibit...

Ngày tải lên: 20/06/2014, 04:20

... IF bonds configuration is different from the film because there are mixed In–As and Ga–As bonds in the IF layer;however, only Ga–As bonds can be found in the buffer and only In–As bonds can be ... positions were occupied by both In atoms and the Ga atoms, and nearly 15% Ga dangling bonds were replaced by In dangling bonds Accordingly, the ratio of Ga–As and In–As dangling bonds became lower than ... occupied by Ga atoms, the IF bonds consisted of both Ga–As and In–As types, and the ratio between them was 2:1 While, when we developed 123 692 Nanoscale Res Lett (2009) 4:689–693 Fig Ball -and- stick...

Ngày tải lên: 22/06/2014, 00:20