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engineering and expression of bibody and tribody constructs in mammalian cells and in the yeast pichia pastoris

báo cáo hóa học:

báo cáo hóa học: " The effect of consequent exposure of stress and dermal application of low doses of chlorpyrifos on the expression of glial fibrillary acidic protein in the hippocampus of adult mice" pptx

Hóa học - Dầu khí

... CPF, alone and in combination, showed increased GFAP expression in offspring in the CA1 sub-field of the hippocampus, and white matter and granular cell layer of the cerebellum [17,18] In the present ... GFAP expression [17,18] The effect of dermal application of CPF on GFAP expression in the hippocampus has not been reported The aim of this study was to determine the expression of GFAP in the ... exposure to nicotine and chlorpyrifos, alone and in combination, leads to persistently elevated expression of glial fibrillary acidic protein in the cerebellum of the offspring in late puberty...
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báo cáo hóa học:

báo cáo hóa học:" Expression of Bone Morphogenetic Protein-2 in the Chondrogenic and Ossifying Sites of Calcific Tendinopathy and Traumatic Tendon Injury Rat Models" doc

Hóa học - Dầu khí

... Activities of BMPs are inhibited extracellularly by BMP-binding proteins such as Noggin and Chordin as well as intracellularly by Smad6, tob and Smurf1 [2] Information on the expression of these osteogenic ... aimed to study the spatial and temporal expression of BMP-2 protein and mRNA in both animal models BMP-2 protein was detected in the chondrocyte-like cells and calcific deposits in both injury models ... activated in both traumatic and collagenase-induced tendon injuries The extent of injury might determine the healed or fail-healing status, consistent with failed healing in tendinopathy was due to the...
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Báo cáo khoa học:

Báo cáo khoa học: "Expression of nitric oxide synthase isoforms in the porcine ovary during follicular development" ppsx

Báo cáo khoa học

... Although the expression of iNOS in porcine ovaries is well known, our findings contrast in part with the previous report [12] In the present study, iNOS was mainly localized to the oocytes of unilaminar ... detected in the granulosa cells of unilaminar follicles (Fig 3B, F, I; Fig 4B) Expression of iNOS was detected in surface epithelial cells (Fig 3C) In the unilaminar, multilaminar, and antral ... observed in the theca of multilaminar follicles (Fig 3H) The expression of nNOS in the theca and granulosa cells of antral follicles (Fig 4A) was strong compared with that in multilaminar follicles...
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Báo cáo khoa học:

Báo cáo khoa học: " Expression of placenta growth factor mRNA in the rat placenta during mid-late pregnancy" pptx

Báo cáo khoa học

... noitazidirbyherp eht gniniatnoc erutxim eht htiw derevoc erew sedils eht ,reffub noitazidirbyherp eht fo lavomer retfA rh rof C 73 ta detabucni dna edimamrof dezinoied %05 gniniatnoc reffub noitazidirbyherp ... '3-GTCGGG AGTACTCGCCGTA-'5 erew ANDc FGlP fo noitacifilpma eht rof desu sremirp editoelcunogilo ehT C ot delooc hsalf dna ,nim rof C 59 ot detaeh ,nim 07 rof C 73 ta dna nim01 rof C 22 ta detabucni erew ... 03 rof C 56 ta CSS × gniniatnoc reffub gnihsaw a ot derrefsnart ,CSS × dna CSS × ni eciwt dehsaw ,nim 01 rof C 73 ta )lm/gµ 05( A esaelcunobir htiw detaert neht erew snoitces eht ,nim 03 rof CSS...
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Báo cáo y học:

Báo cáo y học: "Expression of bioactive bone morphogenetic proteins in the subacromial bursa of patients with chronic degeneration of the rotator cuff" pptx

Báo cáo khoa học

... Hematoxylin-eosin staining (a) and indirect immunofluorescence for collagen types I (b) and II (c) in comparable areas The acromial portion of the areas bursa is in the upper left-hand corner and the ... structures and the acinar elements in the acromial portion (Figure 4) Expression of mRNA for bone morphogenetic protein, matrix proteins, and cytokines Figures and show the results of expression profiles ... aggrecan in this tissue (see below) Within these structures, there were significant deposits of BMP-2 and BMP-7, as visualized by indirect immunofluorescence staining The staining was concentrated in...
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Báo cáo khoa học: Identification in the yeast Pichia stipitis of the first L-rhamnose-1-dehydrogenase gene docx

Báo cáo khoa học: Identification in the yeast Pichia stipitis of the first L-rhamnose-1-dehydrogenase gene docx

Báo cáo khoa học

... the histidine-tag at the N-terminus, we introduced a coding sequence for MHHHHHHGG before the original start codon To introduce the histidine-tag at the C-terminus, we introduced the coding sequence ... trypsination, the peptide masses of the 30 kDa protein were determined by MALDI-TOF MS As the genome sequence of P stipitis is known [12], these masses allowed the identification of the protein ... N-terminally histidine-tagged enzymes were generated in a similar way The coding sequences for six histidines were introduced by PCR either at the N-terminus or at the C-terminus To introduce the...
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báo cáo hóa học:

báo cáo hóa học:" Differential expression of aldehyde dehydrogenase 1a1 (ALDH1) in normal ovary and serous ovarian tumors" docx

Hóa học - Dầu khí

... apoptosis in various cell types including ovary [12-17] In ovarian cancer the expression of the retinol binding proteins involved in RA metabolism is reduced [18] Also it was shown that in the intestine ... surrounding developing follicles did not stain for ALDH1; however, the stromal cells in the perivascular regions and in the developing theca layer of follicles showed ALDH1 staining (Figure 3C and ... localization of ALDH1 in normal ovaries [A-B] Intense staining of numerous cells of stroma (S) and surface epithelial cells (SE) was observed in normal ovary Insets showing examples of ALDH1 stained...
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Báo cáo khoa học:

Báo cáo khoa học: "Immunohistochemical expression of h-telomerase reverse transcriptase in canine and feline meningiomas" pptx

Báo cáo khoa học

... citsalpanA suotamommasP lanoitisnarT citsalborbiF lailehtognineM lailehtognineM lailehtognineM lailehtognineM lailehtognineM lailehtognineM lailehtognineM 21 21 21 21 51 61 21 9 21 11 11 01 01 01 71 )sry( ... lanoitisnart ,)41/1( citsalborbif yb dewollof ,)41/4( citsalpana dna )41/7( lailehtogninem erew devresbo netfo tsom sepytotsih eht ,samoigninem eninac nI stluseR tnacifingis eb ot deredisnoc saw 50.0 < ... uoalocinapaP htiw niatsretnuoC negomorhc BAD sllec lailehtogninem citsalpoen fo )C( msalpotyc dna ,)B( suloelcun ,)A( suelcun eht ni TRET-h fo gniniats lacimehcotsihonummI goD giF noitamrofni lacinilc...
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Báo cáo y học:

Báo cáo y học: "Differential Constitutive and Cytokine-Modulated Expression of Human Toll-like Receptors in Primary Neutrophils, Monocytes, and Macrophages" docx

Báo cáo khoa học

... and GM-CSF did not maintain increased expression of TLR2 as seen after the 3-hour incubation, and the levels of expression had returned to the baseline constitutive expression of unstimulated monocytes ... pro-inflammatory cytokines rather than TLR-ligand binding per se The results of our study demonstrate that several pro-inflammatory cytokines contribute to the regulation of TLR expression Of the cytokines studied, ... to regulate expression of TLRs in leukocytes, including pathogen structures and TLR ligands, such as araLAM and LPS, and pro-inflammatory cytokines, including IL-1β, TNF-α, GM-CSF and IFN-γ.[16,...
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Báo cáo y học:

Báo cáo y học: "Differential expression of chemokine receptors on peripheral blood B cells from patients with rheumatoid arthritis and systemic lupus erythematosus" ppsx

Báo cáo khoa học

... contained large aggregates of B cells, resembling the structure of germinal centers [11,17] These findings suggested that the chemokine CXCL13 is involved in B cell trafficking into the inflamed ... all of the activated B cells in the systemic compartment or these changes in chemokine receptor expression might serve in selective recruitment into the inflamed tissue In line with this interpretation ... with interleukin-2 the expression of CXCR3 is upregulated [32] The observed increase in the frequency of CXCR3hi B cells may result from the chronic activation and differentiation of B cells in...
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Báo cáo y học:

Báo cáo y học: "Expression of CD147 on monocytes/macrophages in rheumatoid arthritis: its potential role in monocyte accumulation and matrix metalloproteinase production" ppt

Báo cáo khoa học

... haematoxylin and eosin staining results of the cells lower surface filters show that the cells had invaded through the filter and attached to the lower side of the filter (× 150) In the co-culture of ... showed positive expression of TIMP-1 in the lining and the sublining layers The expression of CD147 correlated significantly with that of MMP-1 (p < 0.05), but the expression of CD147 and MMP-1 did ... production of MMP-9 and/ or MMP-2 and a significant increase in the number of cells invading through the Matrigel layer and filter compared with those in the respective cultures of these cells alone...
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Báo cáo y học:

Báo cáo y học: "Reactive oxygen species induce expression of vascular endothelial growth factor in chondrocytes and human articular cartilage explants" pps

Báo cáo khoa học

... participating in these processes, especially the surrounding synovial tissue This reflects the findings of Felson and coworkers [27], who declared OA to be a disease involving the whole joint In summary, ... factors in chondrocytes and induce the expression of catabolic cytokines [17] However, the evidence for the role of ROS is conflicting, because other investigators have demonstrated anti-inflammatory ... neutrophil influx and activate MMPs in chondrocytes suggests a central function in catabolic pathways of OA joints [9,25] In addition to the involvement of VEGF in the development of OA, VEGF...
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Báo cáo y học:

Báo cáo y học: "Expression of cartilage-derived morphogenetic protein in human intervertebral discs and its effect on matrix synthesis in degenerate human nucleus pulposus cells" potx

Báo cáo khoa học

... 2) Staining was particularly prominent in the cytoplasm of the chondrocyte-like cells of the NP and IAF, with both single cells and those in clusters showing immunopositivity (Figures and 2) ... vessels and nerves resulting in generation of Here we demonstrate the synthesis and localisation of CDMP and CDMP within human IVDs Although a small decrease in the proportion of cells within the ... staining in the human IVD with a greater number of immunopositive cells within the NP than the IAF and OAF (P < 0.05) Furthermore, in IVDs graded as intermediate degeneration there was an increase...
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Báo cáo y học:

Báo cáo y học: "Altered expression of circadian clock gene, mPer1, in mouse brain and kidney under morphine dependence and withdrawal" pps

Báo cáo khoa học

... stain for determining mPER1 protein expression in brains and kidneys Figure Immunohistochemical stain for determining mPER1 protein expression in brains and kidneys a: Positive staining in the ... is the p-value of circadian rhythm coming from cosine function fitting The hour in the table is the time of clock hour for the acrophase of the fitted cosine function advances the phase of the ... level of mPER1 protein expression in brains was observed at 0:00 Therefore, the phase of the circadian rhythm of mPer1 expression was advanced in mice of the MD group compared with the Con and...
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Báo cáo y học:

Báo cáo y học: "Altered expression of circadian clock gene, mPer1, in mouse brain and kidney under morphine dependence and withdrawa" docx

Báo cáo khoa học

... adjustment of the height of the camera and the adjustment of the angle and distance between the camera and the animal cage The camera was set in such a way that the longest side of the rat cage ... movements in the direction of the camera (it is for this reason that we placed the longest side of the cage in front of the camera) • Part of the bedding that are actively displaced by the rodent during ... from the first module are sent to the standard output: every 25th fraction of a second, a string containing the time and the number of pixels changed, compared to the previous frame, is sent to the...
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báo cáo khoa học:

báo cáo khoa học: "Upregulated expression of indoleamine 2, 3-dioxygenase in CHO cells induces apoptosis of competent T cells and increases proportion of Treg cells" pptx

Báo cáo khoa học

... activity and Tregs in these tumors However, the role of increased IDO expression in tumor cells in development of Treg cells is not clear In this study, we investigated the potential effects of IDO ... increase the proportion of Tregs We also added 1MT, the specific inhibitor of IDO in the co-culture system composing of CHO/IDO cells and CD3+T cells to elucidate the regulatory effect of IDO both in ... T -cells could inhibit Vav1 mRNA and protein expression in T -cells However, an inhibitor of IDO, 1-MT, attenuated CHO/IDO-induced decrease of T-cell proliferation, IL-2 levels in T -cells and inhibition...
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Báo cáo y học:

Báo cáo y học: "Silencing CD36 gene expression results in the inhibition of latent-TGF-β1 activation and suppression of silica-induced lung fibrosis in the rat" pdf

Báo cáo khoa học

... (Stage I) and tiny collagen fibers in the lung of rats in the silica+Lv-shCD36 group Compared to the silica and the silica+Lv-shCD36-NC groups, the number of nodules in the lungs of rats in the silica+Lv-shCD36 ... Lv-shCD36-NC in physiological saline, was instilled into the lungs using a 14-gauge needle inserted into the trachea through the epiglottis of the larynx The site of surgery was sutured and the rats ... could inhibit the expression of the collagen I and III in lung To further observe the degree of fibrosis, immunohistochemical examination of collagen I and III was performed in the lung tissue The...
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Báo cáo y học:

Báo cáo y học: " Expression of Toll-like Receptor 9 in nose, peripheral blood and bone marrow during symptomatic allergic rhinitis" ppsx

Báo cáo khoa học

... Discussion A distinct expression of TLR9 was found in the epithelium, in inflammatory cells in the submucosa, in the endothelial lining and in structural cells in the nose TLR9 expression could ... 8:17 epithelial cell line [30] In the present study, expression of TLR9 was found in the endothelial lining of small blood vessels This finding is in line with the detection of TLR9 mRNA in mouse ... in the apical part of the epithelial lining, in scattered intra- and subepithelial leukocytes and in elongated fibroblast-like cells in the subepithelial tissue (arrow) The epithelial TLR9 immunoreactivity...
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Báo cáo y học:

Báo cáo y học: " Safety and efficacy of undenatured type II collagen in the treatment of osteoarthritis of the knee: a clinical trial"

Y học thưởng thức

... provide written informed consent Use of other natural health products, including glucosamine and chondroitin, one month prior to study and during the study, other than multivitamin and mineral supplements ... capsule contains 375 mg of glucosamine HCl (USP Grade) and 300 mg of chondroitin sulfate (USP Grade) The subjects were instructed to take two G+C capsules in the morning and two in the evening for ... acetaminophen or ibuprofen within days of randomization Subject is unwilling to stop taking pain medication other than the study medication (for arthritis or other types of pain) or is unwilling...
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