deciding when to get married and organizing your timeline

... input, easiness to understand and use, and so forth The most essential factors for choosing the MCA technique is the easiness of understanding by the analyst, the stakeholders and use Ref [16] ... the river, and reduce the opportunities for the ships to pass satisfactorily This necessitates building a decision support tool so as to be used by the construction engineers and contractors This ... criteria Results and discussion All the criteria shown in Figure above have no units at all, so no need to perform the standardisation that has been illustrated into equations and Equations and can be...

Ngày tải lên: 05/09/2013, 16:11

... revert to their default factory settings All static and dynamic addresses will be removed Reset system with factory defaults, [Y]es or [N]o? Enter y and press Enter 6-6 CCNA 3: Switching Basics and ... successfully deleted in Step 2, go to Step and restart the switch using the reload command Software restart (using the reload command) 5-6 CCNA 3: Switching Basics and Intermediate Routing v 3.0 ... Protocol (VTP) information #delete vtp This command resets the switch with VTP parameters set to factory defaults All other parameters will be unchanged Reset system with VTP parameters set to...

Ngày tải lên: 05/11/2013, 12:15

... deeply into the tools What I hope to is to provide enough information so that you can relate your circumstances and issues to the tools and make better decisions as to what is relevant and beneficial ... plants on the other hand tend to be benefit more from RCM, PdM, and maintenance planning and scheduling and less from lean principles and tools, and they typically have an operator to maintainer ratio ... optimizing products, customers, and markets outlined by Christopher^ and Moore^ to better manage those products, customers, and markets and, particularly, to align these to the manufacturing capability...

Ngày tải lên: 04/12/2013, 23:54

... hosts attached to the switches Configure the host to use the same subnet for the address, mask, and default gateway as on the switch Step Verify connectivity a To verify that the hosts and switches ... steps are complete, logoff, by typing exit, and turn all the devices off Then remove and store the cables and adapter 4-5 CCNA 3: Switching Basics and Intermediate Routing v 3.0 - Lab 7.2.4 Copyright ... necessary to force the “other” switch to become the root switch b In the example output given the root switch by default, is Switch_A Switch_B is preferred as the root switch Go to the console and...

Ngày tải lên: 11/12/2013, 14:15

... example will select the top n freight values // Set the field name variable String topNFieldName = FREIGHT_FIELD; int topN = 0; try { topN = Convert.ToInt32(topNTextBox.Text); if(topN

Ngày tải lên: 14/12/2013, 18:16

... Renko et al held together by the disulfide bond between C108 and C119 Its attachment to the body of the enzyme is stabilized by two salt bridges, between H110 and D22, and between R116 and D224 The ... does to cathepsin H To illustrate this, we calculated the average distances between CA atoms of the active site cysteine and histidine residues in cathepsins B and H and the center of CA atoms ... features of the inhibitors as well as to the packing constraints of the environment The larger and wider the features of the ligands that compete with the occluding loop for binding to the active site,...

Ngày tải lên: 18/02/2014, 04:20

... usually represented by the nontoxic ribonuclease T1 [19] The main structural differences between ribotoxins and nontoxic RNases are the length and arrangement of the loops and the N-terminal b-hairpin, ... thought to be related to dissimilarities in loop and the N-terminal b-hairpin, which have been proposed to be specifically involved in vesicle aggregation [21] In order to better understand the ... 0.98 A for all backbone atoms), and the global fold is similar to that reported for cytotoxins However, differences can be found in the conformation of loops, the b-hairpin and the relative position...

Ngày tải lên: 18/02/2014, 08:20

... and L268 and a double mutant of C266 and L268 were successfully constructed and confirmed by DNA sequencing The design of the mutants was intended to preserve the amino acid character and to modify ... approximately 380 and 460 nm and shoulders at 430 and 470 nm The absor- bance maximum of the transition band of the wildtype enzyme at 386 nm was shifted slightly to 381 nm in the C266L, C266M and C266A ... for kcat and Km and the calculated kcat ⁄ Km value for NADPH and Fd are summarized in Tables and L268V FNR displayed kcat and Km values for the diaphorase reaction in the region of 0.8 and 2.0...

Ngày tải lên: 18/02/2014, 17:20

... genes RSc0337 and RSc1501 TH activity was determined at pH and 0.05% SDS, and DO activity at pH and 0.02% SDS genes, which was opposite to that expected from the blast homologies and designated ... a salt gradient of NaCl up to 1.5 m to elute proteins bound to the anionic gel Fractions of 1.9 mL were collected, the protein content was monitored (A280), and TH and DO activities were assayed ... linear gradient of NaCl up to 1.5 M in the same buffer was applied to the column A280, TH and DO stand, respectively, for the profile of UV absorbance (total protein) and enzymatic PPO activities...

Ngày tải lên: 19/02/2014, 07:20

... (Vector Laboratories) and made up to a final volume of mL with NaCl/Pi containing 0.1 M lactose and 2% (v/v) 2-mercaptoethanol This was dialysed for 24 h against L of NaCl/Pi containing 0.1 M lactose, ... variants was compared to wild-type ricin Ricin R213A and ricin R213D were 12-fold and more than 2000fold less cytotoxic than ricin, respectively (Fig 3A) Ricin R258A was equally as cytotoxic as ricin ... (Fig 3B) whereas ricin R258D (Fig 3B) and ricin R48A (Fig 3C) were 10-fold less cytotoxic The reductions in cytotoxcity compared to native ricin were comparable to the decrease in catalytic activity...

Ngày tải lên: 19/02/2014, 12:20

... HoxBC, of R eutropha was shown to be very similar to that of standard hydrogenases, with a Fe atom liganded by CO and CN– [16], and the binding of an hydride to Ni and Fe after H2 reduction has ... provides oxygen tolerance to the soluble [NiFe]-hydrogenase of Ralstonia eutropha H16 by way of a cyanide ligand to nickel J Biol Chem 279, 46686–46689 36 Rey L, Fernandez D, Brito B, Hernando Y, Palacios ... were introduced into the vessel, and the changes in D2, HD and H2 were monitored by scanning masses 4, and 2, 3905 O2 sensitivity of the regulatory hydrogenase HupUV respectively When required,...

Ngày tải lên: 20/02/2014, 01:20

... 0.2 lM to 4000 lM and the ThDP concentrations from 0.04 lM to 200 lM When determining the kinetic parameters for ThDP, the pyruvate concentration was kept at mM Data were analysed and fitted to a ... cofactor ThDP to its binding site The lower thermal stability of the mutant E1s and the increased Vmax in the DCPIP assay, which could be due to easier access of DCPIP to the active site or to ... was from New England Nuclear (Boston, MA, USA) Centrifugal filter devices were purchased from Millipore Bacterial strains and plasmids E coli strain TG1recO and plasmids pKBstE1a and pKBstE1b, expressing...

Ngày tải lên: 20/02/2014, 23:20

... amylose-resin chromatography and treated with factor Xa to generate MBP, SulA3± 169 and SulA23±169 as described previously [12] After digestion of 500 lg of SulA, generated SulA3±169 and SulA23±169 ... SulA3±169, SulA23±169 and maltose binding protein (MBP) The expression vector pMAL-p-SulA was a generous gift from S Sonezaki (Kyushu Institute of Technology, Tobata, Japan) Using the vector, MBP-SulA ... column (250 ´ 4.6 mm, Tosoh Co., Ltd) to separate peptides Each peptide fraction was lyophilized and applied to an amino-acid sequencer (model 477, PE Applied Biosystems Co., Ltd) and an amino-acid...

Ngày tải lên: 21/02/2014, 03:20

... improves the solution and we should increase k We compute the standard deviation of P K2 and use that to establish a boundary as to what it means to be “close enough” to to consider that we have ... of Bill Clinton and Tony Blair with a single name that can refer to either of them In general the names we used in these sets are fairly well known and occur hundreds or even thousands of times ... with and without singular value decomposition, and then second order feature vectors based on bigrams that occurred or more times and had a log–likelihood score of 3.841 or greater, with and without...

Ngày tải lên: 22/02/2014, 02:20

... sites on cytochrome c for cytochrome c oxidase, cytochrome bc1 and cytochrome c1 J Biol Chem 255, 4732–4739 Taha, T.S.M & Ferguson-Miller, S (1992) Interaction of cytochrome c with cytochrome c ... above buffer with gÆL)1 Triton X-100 replacing the dodecyl maltoside To reintroduce this latter detergent for subsequent steps (and to avoid the detrimental effects of Triton X-100 on activity [25]), ... added to the enzyme, induced a decrease in the transition point to biphasic kinetics To some extent, individual effects appear to be additive when following this shift from the four-subunit to the...

Ngày tải lên: 22/02/2014, 07:20

... and their corresponding factor loadings Hence, weight and height contribute positively, and the different skinfold thicknesses (AST, BST, SST, SuST, TST) and MAC contribute negatively to factor ... factors W, H and MAC contribute to the second J Tesedo Nieto et al TST AST BST Second factor Second factor 0.0 SuST SST -0.4 WOMEN AGE -1 MEN W -2 H -0.8 MAC -1.0 -2 -0.5 0.0 -1 0.5 First factor ... subscapular (SST) and suprailiac (SuST) (all in mm), and mid-upper arm circumference (MAC) (cm), were made by the same investigator according to standard techniques of the WHO2 and International...

Ngày tải lên: 05/03/2014, 21:20

... binding of adenosine-5¢-(b,c-imido) triphosphate (AMP-PNP), ATP and MgATP to the ligand-free enzyme To study binding of AdNs to the ligand-free nontagged enzyme, we first measured the change in ITF ... corresponding to the most mobile region (B-factor ‡ 400 A2), ˚ blue corresponding to the most stable region (B-factor £ 40 A2), ˚ and green corresponding to B-factor values in the range 40–400 A2 Note ... that ATP binds to the ligand-free form, and that this also results in changes in the protein conformation ATP binds to the ligand-free open conformation of hGK Previous attempts to demonstrate...

Ngày tải lên: 06/03/2014, 00:20

... phagocytosis 516 Several receptors are involved in opsonization and phagocytosis (e.g the C3b receptor) Receptors for MBL and C1q are also assumed to play a role in opsonization and clearance and ... and to function as a scavenger receptor complex for apoptotic cells and micro-organisms [48– 52] Thus, the calreticulin/CD91 complex has been suggested to recognize C1q and collectins bound to ... process of infectious target/apoptotic cell recognition depends on multiple factors and ligands, and that it has an inherent redundancy, in order to achieve maximal specificity and safety in self/non-self...

Ngày tải lên: 07/03/2014, 05:20

... steady-state level of oxidized enzyme available for inhibitor binding with kynuramine and MPTP [29] Difference spectra and binding curves When ligands bind to the active site of MAO-A, the environment of ... were added to the FAD to reflect the oxidized state of unligated enzyme To give flexibility to the binding site, residues lining the site were allowed to move These residues were: Cys406 (to which ... noncompetitive and mixed [17], or competitive for MAO-A inhibition and mixed for MAO-B inhibition [5] This study examines binding of the imidazoline ligands to purified enzyme of a single subtype (MAO-A) to...

Ngày tải lên: 07/03/2014, 10:20