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ck2α knockdown reversed nuclear translocation of β catenin and altered the expression of e cadherin and vimentin in association with repression of the transcription factors snail1 and smad2 3 expression

Báo cáo sinh học:

Báo cáo sinh học: " Transcription and translation of human F11R gene are required for an initial step of atherogenesis induced by inflammatory cytokines" doc

Hóa học - Dầu khí

... determine directly whether the F11R protein is a critical molecule involved in the adhesion of platelets to endothelial cells, the expression of the F11R gene was silenced in inflamed endothelial ... expression of F11R-mRNA in inflamed endothelial cells We examined whether the observed increases in the level of F11R mRNA in inflamed endothelial cells resulted from the de novo expression of F11R ... were opened in image J, the protein bands were selected using the freeform tool and measured for integrated density The values were normalized to tubulin levels by dividing the integrated density...
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báo cáo hóa học:

báo cáo hóa học:" Effects of alpha-calcitonin gene-related peptide on osteoprotegerin and receptor activator of nuclear factor-B ligand expression in MG-63 osteoblastlike cells exposed to polyethylene particles" doc

Hóa học - Dầu khí

... be identical to the published sequences The b-actin housekeeping gene was used as reference for the relative quantification of the gene of interest, which was expressed as the ratio of ‘concentration ... inverse proportion of the RANKL levels as we believed The detected OPG levels in the cell-particle ratio of 1:500 correspond to the literature whereas the elevation of OPG in the cell-particle ratio ... Subsequently, one-way ANOVA tests were used to determine the detectable change between the groups at each time point One-way ANOVA tests, at each time point relative to the previous time point,...
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báo cáo hóa học:

báo cáo hóa học:" Effects of alpha-calcitonin gene-related peptide on osteoprotegerin and receptor activator of nuclear factor-B ligand expression in MG-63 osteoblastlike cells exposed to polyethylene particles" pot

Hóa học - Dầu khí

... be identical to the published sequences The b-actin housekeeping gene was used as reference for the relative quantification of the gene of interest, which was expressed as the ratio of ‘concentration ... inverse proportion of the RANKL levels as we believed The detected OPG levels in the cell-particle ratio of 1:500 correspond to the literature whereas the elevation of OPG in the cell-particle ratio ... Subsequently, one-way ANOVA tests were used to determine the detectable change between the groups at each time point One-way ANOVA tests, at each time point relative to the previous time point,...
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báo cáo hóa học:

báo cáo hóa học: " Human brain endothelial cells endeavor to immunoregulate CD8 T cells via PD-1 ligand expression in multiple sclerosis" pdf

Toán học

... the CNS We used a well-established in vitro model of the BBB in which HBECs are seeded in the upper compartment of a Boyden chamber [33 ], inflamed, and then incubated with either anti-PD-L1 and ... Blocking PD-L1 and PDL2 in EAE, the mouse model of MS, leads to earlier onset and increased severity of the disease, mainly due to elevated number of infiltrating immune cells, especially CD8 T cells ... been investigated Studies performed in the experimental autoimmune encephalomyelitis (EAE) mouse model of MS have underlined the contribution of PD-1 and its ligands to dampening disease susceptibility...
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Báo cáo khoa học:

Báo cáo khoa học: " Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H2O2-induced oxidative stress" pdf

Báo cáo khoa học

... inhibited the effect of H2O2-induced decrease of cell cycle regulatory protein expression Moreover, H2O2 decreased DNA synthesis However, DHT inhibited the effect of the H2O2-induced decrease of ... time they were washed twice with PBS, then maintained in serum-free DMEM with all supplements and incubated for 24 h The cells were pretreated with or without DHT for 30 and then were treated with ... while keeping a separate count of the blue cells The cells that failed to exclude the dye were considered non-viable; therefore, the data is expressed as a percentage of viable cells Cell injury...
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Báo cáo y học:

Báo cáo y học: " Effect of recombinant IL-10 on cultured fetal rat alveolar type II cells exposed to 65%-hyperoxia" pps

Báo cáo khoa học

... 65%-hyperoxia in the presence and absence of 30 0 ng/ml of rIL-10 were washed, trypsinized and collected into each tube Cells in trypsin were centrifuged at 130 0 rpm for at 4°C, and resuspended in 1X Binding ... Graphical depiction showing IL-8 released into the supernatant in treated and untreated cells The results are represented as the mean ± SD from different experiments Lee and Kim Respiratory Research ... AV-negative cells) expressed as a percentage of the total cell number in treated and untreated cells The results are represented as the mean ± SD from different experiments (D) Graphical depiction...
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Báo cáo y học:

Báo cáo y học: " Gene expression profiling of human prostate cancer stem cells reveals a pro-inflammatory phenotype and the importance of extracellular matrix interactions" pps

Báo cáo khoa học

... by including this data Gene expression signature associated with the cancer stem cell phenotype Following the definition of the cancer stem cell signature, we proceeded to explore the different ... androgen receptor-regulated gene products derived from more abundant differentiated cells and the higher average gene expression in these cells is likely to have masked more subtle expression ... the effects of novel therapeutics on the cancer stem cell population Advances in viable cell separation technology and the first detailed expression signature reported here now provide the means...
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báo cáo hóa học:

báo cáo hóa học: " Increased levels of HMGB1 and pro-inflammatory cytokines in children with febrile seizures" pot

Toán học

... [9 ,34 ] These findings suggest that inflammatory responses in febrile seizures are accentuated by their repetition and increase the likelihood of febrile seizure recurrence Interestingly, no increase ... system and induce the release of catecholamines [31 ,32 ], resulting in cytokine release from peripheral blood mononuclear cells [33 ] However, in our study, patients with recurrent febrile seizure ... role in febrile seizures and epilepsy Since cytokine levels were measured with blood taken 30 after the seizure, the acute effect of seizures could not be distinguished from a persistent inflammatory...
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Motility and alignment of human umbilical vein endothelial cells (HUVEC) on 3d scaffolds

Motility and alignment of human umbilical vein endothelial cells (HUVEC) on 3d scaffolds

Tổng hợp

... all the coordinates of the cell in each frame By using the time interval between every consecutive frames, the speed of the cell can be calculated We regard the center of the shape of the cell ... ridges In these experiments, the results 32 Figure 3. 4: Image of cell movement on the plain surface were taken at different times using different sets of cells (although they were the same type) Inaccuracies ... since if the cells are accumulated together, we can not tell whether the cell moves on its own or is subjected to the interference from other cells Research has indicated that the 30 Figure 3. 3:...
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Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse pdf

Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse pdf

Báo cáo khoa học

... the population represented Lyve-1-positive liver sinusoidal ECs [30 32 ] and maintained the property of Lyve-1 expression in vitro These results also suggest that Lyve-1 expression in liver sinusoidal ... we constructed a transgene driven by the CAG promoter [25] that expresses the b–geo gene [26] in the absence of Cre recombinase, but expresses A new method for mouse endothelial cell culture ... Yamaguchi et al modified mice has accelerated the understanding of genetic mechanisms of endothelial development and functions However, further analyses of vascular phenotypes in vivo have been hampered...
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Tài liệu Báo cáo khoa học: Gene transcription of fgl2 in endothelial cells is controlled by Ets-1 and Oct-1 and requires the presence of both Sp1 and Sp3 pdf

Tài liệu Báo cáo khoa học: Gene transcription of fgl2 in endothelial cells is controlled by Ets-1 and Oct-1 and requires the presence of both Sp1 and Sp3 pdf

Báo cáo khoa học

... constitutive or induced, remain unclear A comprehensive understanding of the general transcription machinery on the native fgl2 promoter is necessary for developing further insight into the TF-independent ... 80%, respectively Mutation of the Ets-1 binding site (mut5) also blocked the effect of Ets-1 in the presence of Sp1/Sp3 Effect of the fgl2 positive regulatory region on induced expression of fgl2 ... variety of genes For example, the expression of endothelial cell-specific TIE2 gene is dependent on Oct-1 and an endothelial cell-restricted cofactor [40] We also identified that the Ets/Stat3 motifs...
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Báo cáo khoa học: Cytosolic phospholipase A2-a and cyclooxygenase-2 localize to intracellular membranes of EA.hy.926 endothelial cells that are distinct from the endoplasmic reticulum and the Golgi apparatus pdf

Báo cáo khoa học: Cytosolic phospholipase A2-a and cyclooxygenase-2 localize to intracellular membranes of EA.hy.926 endothelial cells that are distinct from the endoplasmic reticulum and the Golgi apparatus pdf

Báo cáo khoa học

... both the inner and the outer surfaces of the nuclear membrane In order to assess whether cPLA2-a was relocating to the inner, outer or both surfaces of the nuclear membrane, cells were permeabilized ... in endothelial cells has been reported to occur to sites referred to as the ER, Golgi and nuclear membrane [26,27] However, in very few of these cases have direct double-labelling studies been ... cell The subcellular locations of these proteins also vary, with some being present in the ER, others in the nuclear membrane and some present at both these locations Thus the relocation of cPLA2-a...
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Báo cáo khoa học: Biochemical characterization of human umbilical vein endothelial cell membrane bound acetylcholinesterase ppt

Báo cáo khoa học: Biochemical characterization of human umbilical vein endothelial cell membrane bound acetylcholinesterase ppt

Báo cáo khoa học

... HUVEC process The arrows indicate (a) the percentage of loss between the beginning of the isolation process and the end of the cell lyses; (b) the beginning of the isolation process and after the ... the staining was performed in the presence of eserine 10 lm As expected for the cholinesterase staining, the HUVEC extracts showed multiple bands that were not totally inhibited in presence of ... acetylcholinesterase enzyme present in the membranes of these human cells Several markers can be routinely used to confirm that a given cell culture is of endothelial origin, such as the presence of the...
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Báo cáo khoa học: Lithium increases PGC-1a expression and mitochondrial biogenesis in primary bovine aortic endothelial cells ppt

Báo cáo khoa học: Lithium increases PGC-1a expression and mitochondrial biogenesis in primary bovine aortic endothelial cells ppt

Báo cáo khoa học

... Na-valproate (VPA) for 24 or 36 h After cell lysis, levels of ATP per lg of protein were determined as described in Experimental procedures The graph represents mean ± SEM of the fold increase in treated ... and recent evidence suggests that it might also be beneficial in the treatment of neurodegenerative diseases [6] However, the mechanisms involved in both the beneficial effects and side effects of ... of the CMXRos fluorescent intensity versus the CyQuant fluorescent intensity and the results obtained in eight independent experiments performed in triplicate are presented as the relative MTP levels...
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Báo cáo khoa học: Advanced glycation end products and lipopolysaccharide synergistically stimulate proinflammatory cytokine⁄chemokine production in endothelial cells via activation of both mitogen-activated protein kinases and nuclear factor-jB pdf

Báo cáo khoa học: Advanced glycation end products and lipopolysaccharide synergistically stimulate proinflammatory cytokine⁄chemokine production in endothelial cells via activation of both mitogen-activated protein kinases and nuclear factor-jB pdf

Báo cáo khoa học

... in the development of many diseases that involve elevation of AGEs For example, AGEs are found in the retinal vessels of diabetic subjects, and their levels correlate with the severity of the subject’s ... have a synergistic effect on the induction of cytokines ⁄ chemokines To determine whether there is any combined effect of AGE-HSA and LPS on the induction of cytokines ⁄ chemokines, we stimulated ... subject’s retinopathy [10] Upon the action of AGEs, endothelial cells upregulate adhesion molecule expression and increase proinflammatory cytokine ⁄ chemokine release, resulting in firm adhesion and...
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Báo cáo khóa học: Ouabain stimulates endothelin release and expression in human endothelial cells without inhibiting the sodium pump Robert Saunders and Georgios Scheiner-Bobis pptx

Báo cáo khóa học: Ouabain stimulates endothelin release and expression in human endothelial cells without inhibiting the sodium pump Robert Saunders and Georgios Scheiner-Bobis pptx

Báo cáo khoa học

... models applied over a long period of time causes hypertension [30 ], we investigated here its effects on ET-1 release and expression in HUAECs ET-1, the only member of the endothelin peptide family ... was performed to investigate whether ouabain treatment in uences the sodium pump number on the cell surface The experimental conditions were the same as for the Rb+-uptake experiment After incubation ... oscillations In the best case, four of the six cells observed displayed Ca2+ oscillations The average over three independent experiments was, however, about 38 % In the absence of ouabain, however, control...
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báo cáo hóa học:

báo cáo hóa học:"Interdependency of CEACAM-1, -3, -6, and -8 induced human neutrophil adhesion to endothelial cells" pdf

Hóa học - Dầu khí

... mM final concentration) were added After 30 the wells were washed The * > (panel A) indicates the amount of adhesion observed when neutrophils were incubated for 30 in the presence of buffer containing ... added to the microtiter wells containing the TNF stimulated HUVECs in the absence of Ca2+ (Fig 1, panel A) As expected [37 ,58], when neutrophils were added to the wells in the absence of Ca2+ and ... study CEACAM1 can be expressed on HUVECs Therefore, in earlier studies, a series of experiments addressed the possibility that the observed results could be due to CD66 mAb binding the HUVECs [37 ]...
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báo cáo hóa học:

báo cáo hóa học: " Anandamide inhibits Theiler’s virus induced VCAM-1 in brain endothelial cells and reduces leukocyte transmigration in a model of blood brain barrier by activation of CB1 receptors" pdf

Hóa học - Dầu khí

... CNS represents a key stage in the disease Therefore, here, we investigated the role of CB1 receptors and the effects of the inhibitor of AEA uptake, UCM-707, on VCAM-1 expression in wild type and ... infection of cerebrovascular endothelial cells Thus, infection of endothelial cells might represent one of the first events in the pathogenesis of TMEV-induced demyelination The persistence of TMEV in ... clarify the mechanisms underlying the efficacy of endocannabinoid-bases therapies In this report, we have addressed the role of AEA in regulating 1) VCAM-1 expression in brain endothelial cells infected...
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báo cáo hóa học:

báo cáo hóa học:" Biocompatibility of Poly-ε-caprolactone-hydroxyapatite composite on mouse bone marrow-derived osteoblasts and endothelial cells" doc

Hóa học - Dầu khí

... promising cell source for bone tissue engineering They can be easily harvested, expanded in vitro and induced to differentiate to bone-forming cells [9] We have therefore selected MSCs as the source ... detect the induced endothelial phenotypes The induced ECs were fixed in 4% paraformaldehyde, permeated with 0.01% Triton X-100 in PBS, and incubated in 1% block serum for h at 37 °C The cells were then ... accordance with the manufacturer's instructions The presence of nitric oxide in the culture media of endothelial cells-materials complex was determined by detecting the colored product spectrophotometrically...
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