ca2 signaling and no production in endothelial cells freshly isolated from the luminal surface of the ua ex vivo
Báo cáo khoa học: Advanced glycation end products and lipopolysaccharide synergistically stimulate proinflammatory cytokine⁄chemokine production in endothelial cells via activation of both mitogen-activated protein kinases and nuclear factor-jB pdf
... to the in ammatory loci, which is a key step in inflammation [21,22] Therefore, the cooperation of AGE-HSA and LPS on the induction of cytokines ⁄ chemokines in endothelial cells observed in the ... AGEs and LPS play key roles in the development of in ammatory diseases by stimulating cytokine ⁄ chemokine production from leukocytes and endothelial cells [1,8,9] However, the combined 4602 in ammation-stimulatory ... not shown) AGE-HSA and LPS have a synergistic effect on the induction of cytokines ⁄ chemokines To determine whether there is any combined effect of AGE-HSA and LPS on the induction of cytokines...
- 9
- 409
- 0
Dysfunctional signaling pathway for nitric oxide production in endothelial cells chronically exposed to high glucose or high fatty acids
... therefore interrupted, and NO production is halted The NO formed in endothelial cells diffuses into the subendothelial space and smooth muscle cells, and activates soluble guanylate cyclase in these cells, ... 1.2.2 Endothelial NO production 1.2.2.1 Endothelial nitric oxide synthase and production of NO in endothelial cells There are at least three distinct isoforms of nitric oxide synthase in the body, ... fatty acids on NO signaling pathway in endothelial cells 92 3.2.1 Fatty acids impaired receptor agonist induced NO production in endothelial cells 92 3.2.2 Neither eNOS nor iNOS mass was...
- 171
- 323
- 0
Báo cáo y học: "Nipah virus infection and glycoprotein targeting in endothelial cells" ppsx
... targeting in epithelial cells Neither the di-tyrosine nor the di-leucine motif in the cytoplasmic tail of the G protein influenced G distribution in endothelial cells while the di-tyrosine motif ... and endothelial infection in the CNS is mostly responsible for the pathogenesis of the NiV infection in vivo, we wanted to analyze the spread of NiV in endothelia and to evaluate the role of the ... basolateral G expression in epithelial cells is not required for bipolar expression of G in endothelia Our findings that the Y-based sorting signals in the cytoplasmic tails of F and G not play the same...
- 10
- 366
- 0
Báo cáo y học: "Biochemical and morphological changes in endothelial cells in response to hypoxic interstitial edema" pdf
... distribution of volume and surface of endothelial and epithelial cells in the air-blood barrier Frequency distribution of volume and surface of endothelial and epithelial cells in the air-blood ... considerable thinning of the endothelial layer but no appreciable changes in the epithelial layer Fig C allows to evaluate the response of endothelial and epithelial cells of the air blood barrier in the ... solution) and 40 ml/kg body wt of ketamine injected into an ear vein Subsequent doses of anesthetic were administered during the experiments judging from the arousal of ocular reflexes The study...
- 14
- 240
- 0
Báo cáo y học: "Effects of cigarette smoke on degranulation and NO production by mast cells and epithelial cells" pot
... baseline values) Figure NOx production on the release Effects of nicotinefrom RBL-2H3 of beta-hexosaminidase and Effects of nicotine on the release of beta-hexosaminidase and NOx production from ... seen in the RT-PCR data for the A549 cells above NOS protein expression by Western-Blot and flow cytometry Using flow cytometry to detect iNOS positive cells, the number of cells expressing iNOS ... reduction in nitrite concentration and iNOS expression in a murine lung epithelial cell line in vitro[37] In contrast, Comhair et al showed no change in iNOS expression in airway cells from healthy...
- 9
- 325
- 0
Báo cáo y học: "Hours spent and energy expended in physical activity domains: Results from The Tomorrow Project cohort in Alberta, Canada" pot
... Questionnaire, and reviewed and edited the manuscript NO participated in drafting, reviewing and editing the manuscript PJR participated in the design of the study, and reviewed and edited the manuscript ... our findings of on average a total of hours of reported activity per day from all domains In the CHMS, contextual information was not collected, precluding the exploration of activity and sedentary ... intensity of activities performed Definitions of levels of intensity (1=inactive (mainly sitting); 2=light (mainly standing); 3=moderate (slight increase in heart rate and some light sweating); and...
- 30
- 243
- 0
Tài liệu Báo cáo khoa học: Gene transcription of fgl2 in endothelial cells is controlled by Ets-1 and Oct-1 and requires the presence of both Sp1 and Sp3 pdf
... characterization of the 5¢-flanking region of the mouse fgl2 gene in vascular endothelial cells Through a series of studies focusing on constitutive expression in endothelial cells, we identified that Ets-1 involves ... fgl2 promoter all the way to the beginning of ATG start codon to avoid the discrepancy of minor transcription start variance between macrophages and endothelial cells Endothelial cells transiently ... analyzed as described in Fig in the case of eNOS in vascular endothelial cells [30] To test this hypothesis, we performed an EMSA experiment with a probe containing the fgl2 Sp1 binding site (probe...
- 13
- 525
- 0
Báo cáo y học: "Protein Never in Mitosis Gene A Interacting-1 regulates calpain activity and the degradation of cyclooxygenase-2 in endothelial cells" pot
... porcine μ-calpain, [4-((4-(dimethylamino)phenyl)azo)benzoic acid, succinimidyl ester]-threonine-proline-leucine-lysine~serine-proline-proline-proline-serineproline-arginine-[5-((2-aminoethyl)amino)naphthalene1-sulfonic ... complement of various prostaglandin synthases, and expression of prostaglandin receptors [16] Here, depletion of PIN1 and inhibition of calpain each caused overinduction of both COX-2 and iNOS The consequences ... Figure PIN1 knockdown on calpain and cathepsin activity Effect of8 Effect of PIN1 knockdown on calpain and cathepsin activity Activities were determined from the initial rate of cleavage of fluorogenic...
- 9
- 457
- 0
Brutons tyrosine kinase and protein kinase c µ are required for TLR7 9 induced IKKα and IRF 1 activation and interferon β production in conventional dendritic cells
... important role in TLR7/9 signaling of IFNa production in pDC [10] and IFN-b production in cDC [12] In this paper, we further extended these analyses and sought to delineate the signaling partners ... [45] and other MyD88-dependent TLR signaling of pro-inflammatory cytokine production in CDC [46] However, in all these studies, it is not clear if PKCm is activated during TLR7/9 signaling and ... known to interact with a number of signaling molecules in various immune and cytokine receptor signaling [39] One of these molecules is Protein Kinase C (PKC) [40] Various PKC isoforms are known...
- 9
- 255
- 0
Nitrogen Dynamics and Biomass Production in a Vertical Flow Constructed Wetland Cultivated with Forage Rice and their Mathematical Modeling
... were installed at the upper part of the gravel zone and the water effluent port River water obtained from the Sanno-gawa River was supplied at the top surface of the pot, and it penetrated into the ... the proposed mathematical model Such a constructed wetland can be used not only for the treatment of water but also for the production of biomass The outcomes obtained in the experiment and the ... NH4-N and NO2 +3-N inflow into the ith layer due to advection, respectively The terms rdif,NH,i and rdif ,NO, i are the NH4-N and NO2 +3-N inflow into the ith layer due to diffusion, respectively The...
- 16
- 581
- 1
Tài liệu Báo cáo " Effecting of medium composition on biomass and ginsenoside production in cell suspension culture of Panax vietnamensis Ha et Grushv " doc
... in the medium The maximum production of ginseng saponin and polysaccharide obtained (1.5 g/L and 2.19 g/L) at the initial nitrogen concentration of 40 mM [12] In the simultaneous production of ... callus induction from the cultivated plant root [5] The cell line was maintained in MS liquid medium supplemented with mg/L indole-3-butyric acid (IBA), 0.1 mg/L of kinetin and 30 g/L sucrose The ... Characteristics of the growth and ginsenosides in the suspension culture cells of Korean ginseng (P ginseng C.A Meyer) In W.G Bailey, C Whitehead, J.T.A Proctor, J.T Kyle (eds), Proce Int Ginseng Con.,...
- 6
- 624
- 1
Báo cáo khoa học: Recombinant bovine zona pellucida glycoproteins ZP3 and ZP4 coexpressed in Sf9 cells form a sperm-binding active hetero-complex ppt
... used the Sf9 expression system to obtain each of the bovine rZPGs without the possibility of contamination by the other rZPGs and examined the sperm-binding activity and complex formation of these ... yield of the recombinant proteins; about 15 lg of each rZPG was obtained from 200 mL of culture medium Sperm-binding activity of bovine rZPGs We examined the inhibitory activity of the bovine rZPGs ... S-protein agarose (S) to examine the expression of the rZPGs (B) Immunoprecipitation of rZP3FLAG ⁄ rZP4 mixture individually expressed and then combined Culture supernatants containing rZP4 expressed...
- 16
- 346
- 0
STUDIES ON CATTLE MILK AND MEAT PRODUCTION IN FOGERA WOREDA: PRODUCTION SYSTEMS, CONSTRAINTS AND OPPORTUNITIES FOR DEVELOPMENT doc
... sources of food and income, and dairying has not been fully exploited and promoted The greatest potential for new technologies in dairying is expected in the highlands of Ethiopia and other sub-Saharan ... at a number of sites in the northeastern area of the Lake Turkana basin in Kenya (Barthelme, 1984) After the initial cattle plague (Rinder pest) epidemic and dissemination of the dominant Sanga ... dairy products and beef in the woreda -To assess the production constraints of milk and meat of the woreda and to indicate the interventions for the indicated production constraints LITERATURE...
- 175
- 383
- 1
Báo cáo khoa học: Vaccination with prion peptide-displaying papillomavirus-like particles induces autoantibodies to normal prion protein that interfere with pathologic prion protein production in infected cells pptx
... peptide of nine amino acid residues, DWEDRYYRE, of the murine ⁄ rat prion protein (amino acids 144–152) was incorporated into an immunogenic surface loop of the L1 major capsid protein of bovine ... e.g single amino acid differences from the endogenous PrP sequence (as in the rabbit vaccination), may offer an experimental approach to further evaluate the putative efficacy of PrP–VLP vaccines ... immunoblotting The polyclonal rabbit serum A7 recognized a predominant band of approximately 55 kDa, corresponding to the expected size of the PrP–L1 fusion protein (Fig 1C, left panel), in the...
- 12
- 322
- 0
WASTE MANAGEMENT FROM PULP AND PAPER PRODUCTION IN THE EUROPEAN UNION doc
... be involved in closing the life cycle of their products, from production and throughout their useful life, until they finally become waste The principles of the waste management strategy in the ... operations, in the maintenance of the plant and also in the treatment of fresh water and wastewater 4.2 Papermaking using virgin fibre The waste generated in a paper mill producing paper from virgin pulp ... Horn, 1997) Land application itself consists of transporting the sludges or the wastes, in cake form, from the factory to the fields, and then either spreading them on the land in a thin layer or...
- 50
- 485
- 0
Báo cáo khoa học: Ixocarpalactone A isolated from the Mexican tomatillo shows potent antiproliferative and apoptotic activity in colon cancer cells pot
... to examine the effects of this compound in G2-related proteins These studies revealed an increased expression of pRb with a simultaneous decrease in the expression of retinoblastoma (Rb) in SW480 ... critical role in initiating the apoptotic program by antagonizing the function of the antiapoptotic BCL-2 and activating BAX and BAK [24] Therefore, the expression of BIM ⁄ BOD was evaluated by western ... showed an increased level of expression of pRb and a decreased level of expression of E2F-1 and DP-1 (at 48 h) E2F-1 and DP-1 are known to exist in a complex and act synergistically in E2F site-dependent...
- 10
- 310
- 0
Báo cáo " Effects of macro elements on biomass and ginsenoside production in cell suspension culture of Ngoc Linh ginseng " docx
... for 10-30 min, and then rinsed repeatedly with sterile distilled water The sterilized roots were cut into sections of 2-10 mm and then were inoculated into MS solid medium [7] containing 30 g/L ... concentration of macro elements in the MS medium Cultures were maintained in 300 ml conical flasks for weeks Concentration of macro element NH 4NO3 KNO3 Determination and analyses Extraction and determination ... in the experiment and the inoculum size g/flask (fresh weight) The other cultural conditions were done as described by [9] Results and discussion Effects of macro elements on biomass and ginsenoside...
- 5
- 568
- 0
Báo cáo khoa học: Impact of cyclic hypoxia on HIF-1a regulation in endothelial cells – new insights for anti-tumor treatments doc
... amplification of the HIF-1a response in endothelial cells and how the combination of both inhibitors could in uence the fate of cells exposed to CyH Figure 5A shows that the l-NAME-driven increased ... phenomenon further exacerbated by the increase in O2 consumption in CyH-exposed endothelial cells, (c) the eNOS activation (also triggered by CyH) partly attenuates the HIF-1a increase by interfering ... major in uence of hypoxia However, in tumors, although so-called chronic hypoxia is dependent on the diffusion of O2 and therefore does not in uence endothelial cells located at the beginning of the...
- 10
- 522
- 0
Báo cáo khoa học: Suppression of microtubule dynamics by benomyl decreases tension across kinetochore pairs and induces apoptosis in cancer cells potx
... investigate the status of the checkpoint protein BubR1 following benomyl treatment, we examined the cellular localization of the checkpoint protein BubR1 in the benomyl arrested mitotic cells In the ... indicated that the sister kinetochores are not under tension in the presence of benomyl, resulting in the inhibition of degradation of the checkpoint protein BubR1 at the kinetochore region Benomyl treatment ... resulting in the formation of the kinetochore fibre, leads to a reduction in the concentration of the motor proteins and spindle checkpoint proteins [19] This reduction of checkpoint proteins is...
- 15
- 333
- 0
Báo cáo khoa học: Induction of PAI-1 expression by tumor necrosis factor a in endothelial cells is mediated by its responsive element located in the 4G/5G site ppt
... via the activation of NF-jB Results Upregulation of PAI-1 expression in endothelial cells by ROS In preliminary experiments, we evaluated the role of ROS during the stimulation of PAI-1 expression ... PAI-1 expression in vascular endothelial cells Expression of PAI-1 was analyzed at the level of protein synthesis (A) in the presence or absence of NAC (10 mM) The PAI-1 antigen was determined using ... Thymosin beta induces the synthesis of plasminogen activator inhibitor in cultured endothelial cells and increases its extracellular expression Blood 103, 1319–1324 17 Hou B, Eren M, Painter...
- 11
- 393
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25