... nên tếb o, mô định c/ Tếb o gốc đa (multipotent stem cells) ∗ Là tếb o có khả biệt hóa thành nhiều loại tếb o thể từ tếb o ban đầu ∗ tếb o gốc trưởng thành tếb o gốc tạo máu, tếb o gốc ... b o mầm phôi) - Tếb o gốc thai - Tếb o gốc trưởng thành a/ Tếb o gốc phôi (Embryonic stem cells- ESCs) tếb o mầm phôi (Embryonic germ cells) ∗ Tếb o gốc phôi tếb o gốcvạn lấy từ phôi giai ... hóa cao B Nguồn lấy tếb o gốc ∗ Nguồn lấy tếb o gốc phôi ∗ Nguồn lấy tếb o mầm phôi tếb o gốc thai ∗ 3.Nguồn lấy tếb o gốc trưởng thành 1 Nguồn lấy tếb o gốc phôi: ∗ Tếb o gốc phôi lấy...
... Những tếb o tạo u lành tính tếb o cha đợc biệt hoá tếb o tiền thân biệt hoá B i tránh tạo khối u cách tìm phơng pháp loại b tất tếb o gốcb o thai cha biệt hoá trớc ghép Những tếb o gốcb o ... b o gốc tổ chức phôi, b o thai cá thể trởng thành Những tếb o gốc có nguồn gốc khác nh tếb o gốc phôi (bao gồm tếb o gốc từ phôi tếb o mầm từ tổ chức b o thai) tếb o gốc cá thể trởng thành ... 18 b nạo b Những tếb o máu tuần hoàn mang marker khác với tếb o gan, tuỷ xơng b o thai máu cuống rốn - Tếb o gốcb o thai tếb o mầm b o thai: từ 1985 có nhiều nghiên cứu thu đợc tếb o...
... conditions in mouse embryonic stemcells Methods Mouse embryonic stem cell culture The mouse embryonic stem (MES) cells (CCE-24) were routinely grown on 0.1% gelatin-coated dishes in Dubecco’s Modified ... clearly two bands are visible on the gel with the smaller one (Figure 1B, thick arrow) showing only about 1/20 of the intensity and being about 100bp smaller than the larger band (Figure 1B, thin ... cDNA library in Genbank (Genbank ID: BG970953.1) with the same intron splicing pattern as the truncated form of MsrA cloned from embryonic stem cells, indicating this isoform might not be stem...
... several different retroviral vector backbones The backbones used were pCMV-Babe-OligoRevertible (pCBaOR), pBabe-EGFP-Revertible (pBaER), and pBabe-Oligo-Revertible (pBaOR); these three vectors were ... AB2.2 (WT) NGG5.3 (Blm- /-) Infection Number of colonies Line 1.0 x10 FIAU comment Cell (b) AB2.2 (WT) reports 23 kb 9.4kb 6.5kb deposited research Hind III NGG5.3 (Blm -/-) 23 kb 9.4kb 6.5kb ... producer cells, B4 -5 R48.4 Genome Biology 2007, Volume 8, Issue 4, Article R48 Wang and Bradley http://genomebiology.com/2007/8/4/R48 (b) (a) pWWF1 from pCMV-Babe -oligo-revertible (pCBaOR) CMV-5’TLR...
... chuyên biệt Kết luận chung tếb o gốc: Stemcells 2.PHÂN PHÂNLOẠI LOẠITẾ T B O B OGỐC GỐCStemcells 2.PHÂN PHÂNLOẠI LOẠITẾ T B O B OGỐC GỐCStemcells 2.PHÂN PHÂNLOẠI LOẠITẾ T B O B OGỐC GỐCTếb o ... b o gốc toàn Tếb o gốcvạnTếb o gốc đa Tếb o gốc đơn Stemcells 2.PHÂN PHÂNLOẠI LOẠITẾ T B O B OGỐC GỐC 1/ Tếb o gốc toàn hay tếb o gốc thủy tổ (totipotent stem cells) : Là tếb o có khả biệt ... tếb o mô chết già cỗi tếb o mô Stem cells 2.PHÂN PHÂNLOẠI LOẠITẾ T B O B OGỐC GỐCStemcells 2.PHÂN PHÂNLOẠI LOẠITẾ T B O B OGỐC GỐCTếb o gốc phôi (trong có tếb o gốc phôi thực thụ tếb o...
... Jones JM Embryonic stem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stem cell lines from human blastocysts: ... detached cells were washed off prior to the MTT assay From the raw absorbance values, the survival rate was computed by a simple formula based on reference to the initial absorbance value obtained ... dividing the MTT absorbance values obtained after exposure, with the initial absorbance reading for the unexposed physiological control maintained at 37oC Raw absorbance values obtained for % survival...
... thành hàng vạntếb o theo quy trình đặc biệt Kết kiểm tra hình thái tếb o chất thị hoá mô chứng minh tếb o tách từ phôi chuột tếb o gốc Trước có số nhà nghiên cứu tuyên b tách tếb o gốc phôi ... nghiệm tếb o gốc máu tuỷ xương lách Một số sống tháng Khi mổ chuột đó, nhóm nghiên cứu thấy ổ tạo máu xuất lách chuột Theo TS Hùng, tếb o gốc phôi chuột biệt hoá thành tếb o gốc máu tếb o gốc ... cứu tếb o công nghệ tếb o gốc khác Đối tượng nghiên cứu gà, chuột thỏ Còn nhớ vào đầu năm 2005, thể TS Hùng thành công việc tạo gà khảm vi tiêm tếb o gốc Tách, nhân nuôi khu biệt thành tếb o...
... transcriptional induction of IL-10 by hypoxia ⁄ SD was abolished by the p38 inhibitor SB202190 but was unexpectedly augmented by the proteasomal inhibitor MG132 and by LPS (Fig 7B) Next, the secretion of ... For inhibitor-based studies, 15 lm SB202190 (p38 inhibitor) [36,37], 20 lm U0126 (ERK1 ⁄ inhibitor) [20], 10 lm MG132 (proteasome inhibitor) or lm BAY 11-7082 (NF-jB inhibitor) was preincubated ... MSCs were blocked in 2% goat serum for h and incubated with rabbit anti-(NF-jBp65 primary IgG) for 1–2 h The cells were then washed and incubated with rhodamine-labelled goat anti-(rabbit secondary...
... cells are composed of ES cells and MEF cells grown in ES media containing leukemia inhibitory factor (LIF) These cells express the Rex-1 and b- actin genes but not the embryonic and adult globin ... Failure to detect TBP at the transcribed embryonic globin gene is likely due to the masking of the TBP epitope However, the possibility that TBP is not bound at the promoter can not be ruled out There ... transcription complexes at the murine b- globin locus during the in vitro differentiation of murine embryonic stemcells We observed that elements of the b- globin LCR are capable of recruiting RNA polymerase...
... Epigenetic study of embryonic stemcells Differences in DNA methylation profiles between ES and other stemcells Comparing ES cells with other stemcells established from developing embryos revealed ... 77 ES cells 49 EG cells Embryonic cells Fig Epigenetic distances between ES cells and other stemcells derived from developing embryos ES cells derived from the inner cell mass (ICM) of blastocysts ... important advances in developmental biology and cell biology is the establishment of embryonic stem (ES) cells, which maintain the ability to form all types of cells in the body, and can differentiate...
... Abbreviations ES cells: Embryonic stem cells; VEGF-R2: vascular endothelial growth factor receptor type 2; ECs: endothelial cells; MCs: mural cells; hMNCs: human peripheral blood mononuclear cells; ... Sections were subjected to immunohistochemical analysis with the antibodies for human PECAM-1 (BD Biosciecnces, 1:100), mouse PECAM-1 (BD Bioscience, 1:100), human HLA-A, B, C (BD Biosciecnces, ... 2000, 9:439-443 Brunner G, Nguyen H, Gabrilove J, Rifkin DB, Wilson EL: Basic fibroblast growth factor expression in human bone marrow and peripheral blood cells Blood 1993, 81:631-638 Marcin Majka,...
... rabbit polyclonal IgG anti-galactocerebroside (GalC, 1:100; Santa Cruz) Labeled cells were detected with mouse IgG1 anti-BrdU (1:100; Millipore, Billerica, MA) After thrice washes with PBS, cells ... with 10 μM BrdU in the supplemented culture medium a day prior to nerve fiber transection to rabbits for in vivo study BrdUlabeled cells were then trypsinized and washed thrice with PBS Discrete ... neuroprotective factors produced by the lesioned nerve stumps [14] An ideal scaffold should be biodegradable, biocompatible, non-toxic and mediate no immune response In general, these biomaterials yielded...
... http://www.translational-medicine.com/content/7/1/20 A B C D supervised clustering (A) Adult cells and EB shared genes (B) hES cells upregulated genes (C) EB upregulated genes (D) Adult cells upregulated genes hESC cells EB adult cells Figure ... miR-200c, miR-30 2b, and miR-367 between hES cells and EB, and between hES cells and adult cells was significant (P < 0.05) The difference in miR-302c expression between hES cells and adult cells was ... in EB than in either hES cells or adult cells (Figure 6, panel B) For miR-10 6b, miR-92, miR-93, miR-130a and miR-190, the difference in their expression between EB and hES cells and between EB...
... Platt JL: Immunosuppression by embryonic stemcellsStemCells 2008, 26(1):89-98 Fabricius D, Bonde S, Zavazava N: Induction of stable mixed chimerism by embryonic stemcells requires functional ... globin genes expression in blast colony (BC), BFU-E and in undifferentiated hES cells (negative control) Endothelial potential of blast cells Based on the definition of hemangioblast, blast cells ... to both blood and endothelial progenitors Similar to the above publications, we performed hES cell differentiation in EB system and obtained blast colonies which were further shown to be bipotential...
... Multipotent stemcells from umbilical cord: cord is richer than blood! StemCells 2008, 26(1):146-150 Zhang MJ, Liu B, Xia W, Sun ZY, Lu KH: Could cells from menstrual blood be a new source for cell-based ... hFTs cells, different cell types were observed but most were spindle-shaped, resembling fibroblasts Some clusters of cells with endothelial appearance, which spread weakly, could also be observed ... G, Sbarbati A, Bonetti B: Induction of neural-like differentiation in human mesenchymal stemcells derived from bone marrow, fat, spleen and thymus Bone 2007, 40:382-390 Prunet-Marcassus B, Cousin...
... this source of stemcells has not been used for transplantation in HIV patients yet Cord blood may emerge as an important source of CCR5-negative stemcells because they have been used successfully, ... from bone marrow donor registries (ZKRD Ulm, Germany, and BBMR, Bristol and London, UK) and donor centres (DKMS, Tübingen, Germany, and Stefan-Morsch-Stiftung, Birkenfeld, Germany) met at a Berlin ... CCR5-negative stemcells is the donor information policy of the bone marrow registries Transplantation from units of umbilical cord blood stem cell only requires 4/6 HLA matches at HLA A, B and DR...
... chuột khảm chứng tỏ tếb o iPS có tính chất không khác tếb o gốc phôi Các nghiên cứu công b tuần ba nhóm nghiên cứu khác cho thấy tái lập trình tếb o da b nh thường thành tếb o gốc phôi chuột ... kích hoạt biểu gene khác, làm cho tếb o trở thành vạn năng, tức chúng có khả trở thành b t kỳ tếb o thể Yamanaka gọi chúng tếb o gốcvạn cảm ứng (iPS cells) "Dễ b n Chẳng có phép màu cả." Yamanaka ... trình bao gồm việc thu trứng chưa thụ tinh, thay vật chất di truyền trứng vật chất di truyền từ tếb o b nh nhân ép cho tếb o phân chia để tạo phôi, từ thu tếb o gốc phôi Những rào cản b xóa b ...