... 1,GST -C2 domain without cardosin A; lane 2, cardosin A incubatedwith GST -C2 fusion protein; lane 3, cardosin A incubated with GST- C2 fusion protein in the presence of pepstatin A; lane 4, GST -C2 domain ... the recognition of the C2 domain.The C2 domain is degraded by cardosin A aftercomplex disruptionAfter establishing the importance of RGD-likesequences in cardosin A C2 domain complex forma-tion ... complexdisruption resulted in C2 domain cleavage byFig. 5. Cardosin A interacts with the C2 domain of PLDa. Pull-downassays for cardosins A and B were performed with GST -C2 domainfusion protein....