Báo cáo khoa học: Does different orientation of the methoxy groups of ubiquinone-10 in the reaction centre of Rhodobacter sphaeroides cause different binding at QA and QB? potx
Báo cáo khoa học: A single EF-hand isolated from STIM1 forms dimer in the absence and presence of Ca2+ ppt
... lm). The binding of Ca 2+ resulted in a more compact conformation of the engineered protein. Our results also showed that D to A mutations at Ca 2+ -coordinating loop positions 1 and 3 of the ... between the two termini of the inserted Ca 2+ -binding sites in the model structure of the EF-hand of STIM1 is within 15 A ˚ . Accordingly, a total of six glyc...
Ngày tải lên: 07/03/2014, 00:20
Báo cáo khoa học: Kinetic studies and molecular modelling attribute a crucial role in the specificity and stereoselectivity of penicillin acylase to the pair ArgA145-ArgB263 pdf
... that the ArgB263 residue could be involved both in catalysis and productive binding. Graphic simulation of the interactions of arylamide substrates with the nucleophile binding subsite of PA indicates ... of the side chain d-guanidino group of this residue are involved in H-bonding as follows: Ne with the O atom of the main chain CO group of the TrpB240;...
Ngày tải lên: 16/03/2014, 16:20
Báo cáo khoa học: Superoxide radical-scavenging effects from polymorphonuclear leukocytes and toxicity in human cell lines of newly synthesized organic selenium compounds potx
... concentration of the oxidant in the samples was calculated using the standard curve, which was made by adding known concentrations of the authentic H 2 O 2 instead of the samples. Visualization of ... containing 1mm NaN 3 , and then incubated at 37 °C in an atmosphere of 95% air and 5% CO 2 in the presence of each organic selenium compound or SOD. After 5 m...
Ngày tải lên: 23/03/2014, 07:20
Báo cáo khoa học: Novel diadenosine polyphosphate analogs with oxymethylene bridges replacing oxygen in the polyphosphate chain pdf
... could arise either from weaker binding of 3 in the active site of the hydrolase in the reverse orientation (Fig. 3B) or from a reduced rate of cleavage. While A B Fig. 2. Time course of ApOpCH 2 OpOpA ... reduction in rate of about 10-fold for the formation of 18 relative to that of 32. Thus, the present kinetic results do not provide any evidence for differe...
Ngày tải lên: 16/03/2014, 04:20
Báo cáo khoa học: NrpRII mediates contacts between NrpRI and general transcription factors in the archaeon ¨ Methanosarcina mazei Go1 pot
... whereas no binding at all was obtained in the presence of 10 mm 2-oxo- glutarate (Fig. 3C bottom panel, lanes 1–6; and Fig. 3D). Next, binding of NrpRI to the nifH promoter in the presence of NrpRII ... verifying formation of a complex between NrpRI and NrpRII. The presence of 10 mm 2-oxoglutarate significantly decreased the binding of MBP–NrpRI to the o...
Ngày tải lên: 29/03/2014, 21:20
Báo cáo khoa học: Relaxin-3⁄ insulin-like peptide 7, a neuropeptide involved in the stress response and food intake Masaki Tanaka pptx
... including the stress response and food intake, are described. Expression of relaxin-3 in the brain Relaxin-3 neurons in the brain Examination of relaxin-3 mRNA expression by north- ern blotting ... libitum-fed rats [32]. After such chronic administration, the plasma concentration of leptin and insulin was significantly increased [32]. In addition to the PVN, relaxin-3...
Ngày tải lên: 29/03/2014, 21:20
Báo cáo khoa học: Does different orientation of the methoxy groups of ubiquinone-10 in the reaction centre of Rhodobacter sphaeroides cause different binding at QA and QB? potx
... Does different orientation of the methoxy groups of ubiquinone-10 in the reaction centre of Rhodobacter sphaeroides cause different binding at Q A and Q B ? Andre ´ Remy 1 , ... at either the Q A or the Q B binding site. Two infrared bands at 1288 cm )1 and 1264 cm )1 were assigned to the methoxy vibrations. They did not shift in fre...
Ngày tải lên: 23/03/2014, 21:20
Tài liệu Báo cáo khoa học: A novel coupled enzyme assay reveals an enzyme responsible for the deamination of a chemically unstable intermediate in the metabolic pathway of 4-amino-3-hydroxybenzoic acid inBordetellasp. strain 10d doc
... analysis, and the determination of released ammonia. The coupled enzyme assay revealed the mechanism of the deamination reaction and the subsequent metabolism, including the deamination step. The product ... to identify the cofactor of the deaminase from strain 10d because the enzyme could not be purified in large enou gh quantities. We previously reported th...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: Experimental proof for a signal peptidase I like activity in Mycoplasma pneumoniae, but absence of a gene encoding a conserved bacterial type I SPase pdf
... at its N-terminus instead of a free amino group [32]. Therefore, the asparagine in position 26 was labeled within the intact protein at its a-amino group and represents the amino terminal amino ... terminus of P40. Knowing, that the first amino acids of the processed P40 and P90 is the asparagine at position 26 and the arginine at posi- tion 455 we calculate...
Ngày tải lên: 19/02/2014, 18:20
Tài liệu Báo cáo khoa học: "STRING-TREE CORRESPONDENCE GRAMMAR: A DECLARATIVE GRAMMAR FORMALISM FOR DEFINING THE CORRESPONDENCE BETWEEN STRINGS OF TERMS AND TREE STRUCTURES" pdf
... string of terms and a structural representation is defined as a mapping between elements of the set of substrings of the string and elements of the set of subtrees of the tree : such a relation ... also note that the nodes in the TREE are simply concepts in the structural representation and thus the interpretation is independent of any grammar that...
Ngày tải lên: 22/02/2014, 10:20