Báo cáo Y học: Synthesis, characterization and application of two nucleoside triphosphate analogues, GTPcNH2 and GTPcF pdf
Báo cáo Y học: Synthesis, characterization and application of two nucleoside triphosphate analogues, GTPcNH2 and GTPcF pdf
... function, structure and mechanism, often demands use of nonhy- drolyzable or slowly hydrolyzable nucleotide analogues. These modifications become necessary when stabilization of a specific isoform of the protein ... rate of hydrolysis of GTPcNH 2 bound to Ras protein lay between the rates found for Ras-bound GTPcS and GppNHp, while Ras-catalysed hydrolysis of GTPcF was almost...
Ngày tải lên: 18/03/2014, 01:20
Báo cáo y học: " Polysaccharides from the root of Angelica sinensis protect bone marrow and gastrointestinal tissues against the cytotoxicity of cyclophosphamide in mice
... Immunology and Department of Pharmacology, Faculty of Medicine, The University of Hong Kong, Hong Kong, China Corresponding address: Prof. C.H. Cho, Department of Pharmacology, The University of ... subcutaneously once daily) on white blood cell (WBC) number in cyclophosphamide (CY)-treated mice. CY was given subcutaneously (200 mg/kg) at day 0 and day 7 and AP was also i...
Ngày tải lên: 02/11/2012, 10:19
Báo cáo Y học: Spectroscopic characterization and ligand-binding properties of chlorite dismutase from the chlorate respiring bacterial strain GR-1 ppt
... 0.3 and typically yielded 70–100 g wet cells. The anaerobicity of the culture was indicated by decolorization of the redox indicator Correspondence to P. L. Hagedoorn, Kluyver Department of Biotechnology, ... 7.16 and rhombicity V/D ¼ 0.52), horseradish peroxidase (5.15 and 0.38), cytochrome c peroxidase (7.29 and 0.49), myoglobin (6.92 and 0.46), and hemoglobin (6.61 and...
Ngày tải lên: 08/03/2014, 16:20
Báo cáo Y học: Synthesis and turn-over of the replicative Cdc6 protein during the HeLa cell cycle potx
... (0–2 h after thymidine-block) and in the middle of S phase (4–6 h), as well as at the e nd of mitosis and during the early G1 phase (12–14 h) of the next cycle (Fig. 2A). Cytoplasmic and chromatin ... hCdc6p is synthesized at similar rates during various stages o f t he cell cycle, and determined a half life of newly synthesized hCdc6p of 2–4 h in S phase. The data suggest a...
Ngày tải lên: 17/03/2014, 23:20
Báo cáo Y học: Synthesis of phosphoenol pyruvate (PEP) analogues and evaluation as inhibitors of PEP-utilizing enzymes pot
... treatment of 6e and 7a with trimeth- ylsilyl bromide and methanolysis. Finally, the ethyl ester group of 8a was hydrolysed under basic conditions, and the Z-andE-isomers were separated by anion-exchange chromatography. ... inhibitor of PEP mutase [36], and pyruvate kinase [6]. Inhibition of pyruvate kinase. This enzyme catalyses the regeneration of ATP from ADP and PEP, in...
Ngày tải lên: 18/03/2014, 01:20
Báo cáo Y học: Synthesis, conformational analysis and biological activity of cyclic analogs of the octadecaneuropeptide ODN Design of a potent endozepine antagonist pot
... secondary structure of the cyclic OP analogs by two- dimensional 1 H-NMR and molecular dynamic simulation, and we have investigated the biological activity of these analogs by measuring their ability ... of experiments [25]. RESULTS Synthesis of cyclic peptides The two cyclic peptides cyclo 128 OPandcyclo 128 [D-Leu5]OP were synthesized directly on solid support and the head-...
Ngày tải lên: 24/03/2014, 04:21
Báo cáo Y học: The cold-active lipase of Pseudomonas fragi Heterologous expression, biochemical characterization and molecular modeling pdf
... peptide-independent pathway and calls for further investigation on this subject. Enzyme activity and specificity In the standard tricaprylin hydrolysis assay, rPFL displayed highest activity at 29 °C and pH 8.0, ... molecular basis of cold activity and the interplay between flexibility and catalytic efficiency areof importance inthe study ofstructure–function relationships in protein...
Ngày tải lên: 24/03/2014, 04:21
Báo cáo Y học: Molecular characterization of MRG19 of Saccharomyces cerevisiae Implication in the regulation of galactose and nonfermentable carbon source utilization pdf
... of galactose and nonfermentable carbon source utilization Firdous A. Khanday*, Maitreyi Saha and Paike Jayadeva Bhat Laboratory of Molecular Genetics, Biotechnology Center, Indian Institute of ... possibility by measuring b-galactosidase activity driven by ADH2 and CTA1 promoters in wild-type and MRG19 disruptant at low and high cell density. Both of these promoters are unde...
Ngày tải lên: 31/03/2014, 08:20
Báo cáo y học: "A prospective observational study of the relationship of critical illness associated hyperglycaemia in medical ICU patients and subsequent development of type 2 diabetes"
... differences in age and sex distribution. Patients in the hyperglycaemia group had a higher proportion of positive family history of diabetes and higher body mass indexes. During the five years of follow-up, ... development of hyperglycaemia, but not all severely ill develop hyperglycemia and some do even in mild disease. We hypothesised that acute disease only exposes a latent...
Ngày tải lên: 25/10/2012, 10:02
Báo cáo y học: "Strict glycaemic control in patients hospitalised in a mixed medical and surgical intensive care unit: a randomised clinical trial"
... admission. They were also measured daily in the mornings. The median of all daily values and daily maximal and minimal blood glucose levels were documented. Hypoglycaemic episodes of less than ... mortality at 28 days was 32.4% (81 of 250) in the standard insulin group and 36.6% (93 of 254) in the intensive insulin group. ICU mortality was similar for patients of the standard...
Ngày tải lên: 25/10/2012, 10:35