Báo cáo khoa học: Interaction of the C1 complex of Complement with sulfated polysaccharide and DNA probed by single molecule fluorescence microscopy pdf

... Interaction of the C1 complex of Complement with sulfated polysaccharide and DNA probed by single molecule fluorescence microscopy Be ´ range ` re Tissot 1 ,Re ´ gis Daniel 1 and Christophe ... bind to DNA strands in the absence of C1q (data not shown). Therefore the binding of C1r is a direct consequence of the presence of C1q on DNA s...

Ngày tải lên: 16/03/2014, 23:20

... polysaccharide, pullulan. Comparison of the active sites of the model of IPU and Dex49A The active site structures of t he model of IPU (Fig. 4A) and Dex49A (Fig. 4B) were compared. The three Asp residues, Asp353, Asp372 and Asp373, ... as the substrate. The anomeric forms of t he hydrolysate are equilibrated immediately by the addition of ammonium hydrox...

Ngày tải lên: 19/02/2014, 16:20

... (Figures 3 and 4), we find: (i) Examination of 50% of the data in the SLs leads to identification of between 75% (AdjN) and 80% (PNV) of the TPs. (ii) For the first 40% of the SLs, and lead to the worst results, ... fre- quency) and the weak measures ( and ). In the second half of the data in the SLs, how- ever, there is virtually no difference between ,...

Ngày tải lên: 20/02/2014, 18:20

... fluorescence heterogeneity with specific coupling Fig. 5. Binding of Ant-dATP to AC (d) and AC–CaM (m). The degree of binding of the ligand was calculated from the value of the ampli- tude of the ... the uncomplexed AC and the AC–CaM complex. Dynamics of the AC catalytic domain as probed by acrylodan Taking advantage of the absence of Cys residu...

Ngày tải lên: 07/03/2014, 15:20

... 10 mm). The structural identity of the isolated analogs was con- firmed by MALDI-TOF ⁄ TOF MS, with a 4800 MALDI- TOF ⁄ TOF Analyzer (AB SCIEX, Framingham, MA, USA). MALDI-TOF and TOF ⁄ TOF MS spectra ... Cys345 and the thiol form of bacitracin A. The thiol form of the thiazoline ring of bacitracin A and Cys345 are shown as chemical structures, the rest of bacit...

Ngày tải lên: 14/03/2014, 23:20

... consisting of V H and V L regions with ⁄ without the V H leader sequence at the N-terminal and with ⁄ without the C L (j) region behind the V L region. None of the con- structs contained the KDEL ... j and IgG2b ⁄ j, respectively. The appropriate cDNA fragments of the V H and V L regions were then generated by RT-PCR. A compar- ison of the V H and V L...

Ngày tải lên: 16/03/2014, 14:20

... controlled by the kinases or phosphatases in the system, and whether they are controlled differ- entially by some or all of them. On the basis of the antagonistic action of the kinases and phosphatases one ... to how these time patterns are controlled by the kinases and the phosphatases. In order to examine how the second kinase in the cascade determines t...

Ngày tải lên: 16/03/2014, 18:20

... compute a linear regression of Q-level across 12 months, conduct a hypothesis test (with the null hypothesis being the slope of the regression equal to zero), and report the P -value for two-tailed ... for by Q-queries. We plot this against b’s activity level, measured by the number of queries issued by b in a month. We binned users by their activity levels on the...

Ngày tải lên: 17/03/2014, 00:20

... conformation and hydrogen bonding network of the pterin cofactor in the three structures. Superpositions of the binary and ternary complexes of hPAH onto the crystal structure of the ligand-free ... changes of the pro- tomer resulting in a displacement of the cofactor bound at the active site by 2.6 A ˚ (mean distance) in the direction of the iron and...

Ngày tải lên: 17/03/2014, 09:20

... like the newly reported G90V adRP mutation [19]. The G51A, G51V and G89D mutants were studied in the context of the folding and packing of the TM domain together with other adRP mutations in the ... dramatically influenced by pH. Rate of Meta II decay as measured by retinal release The rate of retinal release, which parallels the Meta II decay of the prote...

Ngày tải lên: 22/03/2014, 16:20