Báo cáo khoa học: The optimization of protein secondary structure determination with infrared and circular dichroism spectra docx

... aB-crystallin (i.e. the effect of the additive on the aggregation of the target protein is inde- pendent and different from that of the chaperone). The standard errors associated with this theoretical ... against this target protein. The effect of Arg-HCl on the structure and assembly of aB-crystallin We investigated whether the effects of these additi...

Ngày tải lên: 18/02/2014, 16:20

... and the effect of the absence of intact complex I, but the results are too preliminary in this regard. Heterologous expression of the ESSS-HA subunit The localization of the ESSS subunit in the ... revealed the existence of two additional subunits (bringing the total to 46), and one of these, the ESSS protein, is also encoded by an X-linked g ene in humans...

Ngày tải lên: 19/02/2014, 16:20

... required for efficient cleavage [11]. The solution structure of the SEA domain of the mouse homologue of MUC16 (which in the human encodes the core protein of the ovarian cancer antigen CA125) was ... MUC16 and MUC17 and other proteins with cleaved SEA domains (Table 1). The SEA module of rat MUC3 is cleaved soon after translation in vivo and in vitro at th...

Ngày tải lên: 19/02/2014, 18:20

... substitution of residue 71 We measured the CD and fluorescence spectra of SGT-L71Y, SOT-Y71L, SOT and SGT to investigate the effect of the mutation at residue 71 on the tertiary and secondary structures of ... sche- matically. The recombination sites of chimeras A–H are shown in (B). The primary structures of SOT (upper) and SGT (lower) are shown. The reco...

Ngày tải lên: 16/03/2014, 00:20

... nmol or 0.66 nmol of rMIH-B at the arthropodial membrane of the periopod and returned to the culture tanks. At 24, 48 and 72 h after injection, the hepatopan- creas and ovary of the shrimp were ... females. The percentage indicates the GSI of the females. M (B) indicates the expression pattern of MIH-B in the same tissues in males (N ¼ 5). The lower panel...

Ngày tải lên: 16/03/2014, 05:20

... disruption of the association of these proteins could represent a poten- tial therapeutic strategy for the treatment of AD. Here, we used GST fusion proteins of Fyn-SH2 and Fyn- SH3 to further investigate ... wild-type and all of the mutant forms of tau except for YallF and Y394F tau (Fig. 3). In contrast to the results obtained with Fyn-SH2, wild-type tau and al...

Ngày tải lên: 14/02/2014, 14:20

... induce a massive accumulation of the reporter protein in the cytoplasm, exceeding that of the reporter protein fused with the full-length ASF ⁄ SF2 protein, or with the RRM1 alone. Most likely, ... upon expression of CP alone. The tethering of TTP led to a decrease of 8MS2 Rluc reporter mRNA to the same extent as that at the protein level (Fig. 7E), theref...

Ngày tải lên: 16/02/2014, 15:20

... that open to the atmosphere on the cuticle and radiate to all parts of the body. O 2 is delivered through trachea and trache- oles in the gaseous phase [9] and hence respiratory proteins have ... possible ‘intermediate’. The evolution of hemocyanin subunits follows the widely accepted phylogeny of the Hexapoda and provides strong evidence for the monophyly of...

Ngày tải lên: 18/02/2014, 08:20

... consists of the entire chroma- tin of the nucleated avian erythrocytes and comprises approximately 85% of the chromatin in other cell types [5]. The structure of chicken erythrocyte chromatin is the ... on the relationship with respect to nucleosome orientation and tilt versus chromatin repeat length. Thus, they differ with respect to the ori- entation of th...

Ngày tải lên: 18/02/2014, 18:20

... associated with the intercalative conformation of the (+)-cis adduct and the minor- groove conformation of the (+)-trans adduct, the lat- ter interfering with interactions of the catalytic loops of the ... accordance with this, the depend- ence of the fluorescence of the (+)-trans adduct on its position relative to the target dC was revealed in the c...

Ngày tải lên: 19/02/2014, 00:20