Tài liệu Báo cáo khoa học: Bioinformatic and enzymatic characterization of the MAPEG superfamily doc

... from the archaea kingdom, indicating that the enzymatic activities of the MAPEG family are not present in these species or that these activities are catalysed by other enzymes. The absence of MAPEG members ... [57]. Isolation and cloning of the SynMGST and E.coliMGST The coding sequence for the SynMGST, corresponding to the complementary strand of the nucle...

Ngày tải lên: 19/02/2014, 17:20

... disappearance of the heavier inactive peak. Furthermore, FPLC analysis of the recombinant wild-type cN-II showed the presence of the tetrameric active form irrespective of the pres- ence of effectors. ... [9]. For determination of kinetic parameters (K m and k cat ), the concentration of the labelled substrates ranged from 0.02 to 4mm. A plot of the dependenc...

Ngày tải lên: 15/02/2014, 01:20

... spectrophotometric methods. Because the enzymatic activity of PRTFDC1 towards hypoxanthine and guanine is only 0.26% and 0.09%, respectively, of the activity of HPRT, the role of the PRTFDC1 in purine metabolism ... II. The hood domain is mainly built up by residues from the C-terminus and consists of a two-stranded anti-parallel b-sheet composed of b2 and b9...

Ngày tải lên: 15/02/2014, 01:20

... performed using T-COFFEE [44]. Docking simulation of substrate on the active site of HYD Js The substrate binding pockets of HYD Js and the mode of interaction between substrate and enzyme were ... A3(2). Cloning and expression of the putative hyd genes The deduced open reading frames of putative hyd genes were PCR-amplified from the genomic DNA of the corresp...

Ngày tải lên: 18/02/2014, 08:20

... is the measured anisotropy value, A F is the aniso- tropy of the free minifiber, A B is the anisotropy of the mini- fiber bound to the penton base, [Lig B ] is the concentration of the bound ligand ... motif that acts as a trigger for endocytosis of the adenovirus into the host cell. Furthermore, the architecture of the entire virus, including the positions...

Ngày tải lên: 19/02/2014, 06:20

... and ^ represent parallel and perpendicular polarized light, respectively. The bottom panel displays the result of deconvolution of the IR absorption spectrum of the peptide f or analysis of the ... IFP of ASLV-A and its membrane interactions to examine the difference in the biophysical property b etween the N-terminal and internal FPs, in the hope of impr...

Ngày tải lên: 19/02/2014, 16:20

... (DEV) and a test setting (TEST). In the development setting, we run the typical 5 fold cross validation where we train on 4 folds and test on the 5th and then average the re- sults. In the test ... set and Annotation Corpus: Two college-educated native speakers of Arabic annotated 2855 sentences from Part 1 V 3.0 of the PATB. The sentences make up the first 400 docu...

Ngày tải lên: 20/02/2014, 05:20

... prediction of theoretical three- dimensional structure of the enzyme. The three different views were obtained by simple rotation of the same model. The flexible LID domain appears at top of the three ... open and closed conformation to the protein during binding of the substrate is shown in the top-most portion of the protein. ATP may bind to the glycine loop o...

Ngày tải lên: 21/02/2014, 00:20

... products of the hazelnut LOX were compared with the reaction products of soybean LOX type I and authentic standards of 9- and 13-HODE. As shown in Fig. 4, 9-HODE is the main product of the hazelnut ... position 4235 of the genomic clone as the translation start. The characterization of other clones revealed the presence of another cDNA (CaLOX2) sharing high...

Ngày tải lên: 21/02/2014, 00:20

... over the course of the decade, although it appears that there is a lack of awareness of the fact that a documentation of the computational approaches is scientifically as important as the docu- mentation ... developments on the theoretical side layed the foundation of the field, such as metabolic control analysis [4,5] and biochemical systems theory [6]. In the 198...

Ngày tải lên: 14/02/2014, 14:20